D5GHP2 (AMPP2_TUBMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable Xaa-Pro aminopeptidase GSTUM_00008071001
EC=3.4.11.9

Alternative name(s):
Aminoacylproline aminopeptidase
Prolidase

Gene names

ORF Names:

GSTUM_00008071001

Organism

Tuber melanosporum (strain Mel28) (Perigord black truffle) [Reference proteome]

Taxonomic identifier

656061 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › Pezizomycetes › Pezizales › Tuberaceae › Tuber › Tuber melanosporum

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides By similarity.
Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Sequence similarities	Belongs to the peptidase M24B family.

Ontologies

Keywords
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Probable Xaa-Pro aminopeptidase GSTUM_00008071001		PRO_0000411855
Sites
	Metal binding	251	1	Manganese 2 By similarity
	Metal binding	262	1	Manganese 1 By similarity
	Metal binding	262	1	Manganese 2 By similarity
	Metal binding	386	1	Manganese 1 By similarity
	Metal binding	426	1	Manganese 1 By similarity
	Metal binding	426	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	D5GHP2 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: BB374E5FB69A7739	FASTA	455	50,793
	10 20 30 40 50 60 MTSDSTVDKI LAGKYPAKQH AENVVERLLH VHPDLTDGVI YLESQRSKLY ENSDQEVPFR 70 80 90 100 110 120 QRRYFYYLSG CDLADSYLTY SIRDRKLTLF IPPIDPASVL WSGLPLSNSE ALEKYDVDEV 130 140 150 160 170 180 LPTSATALPT TSYSSLMFVI ESQTSRTFHL QNTESLEPAI ERARAIKDEY EVALIKKANR 190 200 210 220 230 240 ISALAHHSCL RAIKSAGNER EIEAVFTKEC IANGAPKQAY SGIFGSGRSA STLHYVHNNQ 250 260 270 280 290 300 PLAGKLNLLL DAGAEYNNYA SDITRTFPIS GQFTKESREV YDIVLDMQKQ CLAASKAGAV 310 320 330 340 350 360 WDDIHILAHK VAIQGLLKIG VLRNGSVDEI LSNRTSTAFL PHGLGHYLGM DTHDCGGNPN 370 380 390 400 410 420 YADPDPMFKY LRKRGPLPAG AVITVEPGIY FCEFIIKPYL EDEKHAKYID KDVLNRYWDV 430 440 450 GGVRIEDNIL ITEGGYENLT NVAKEVDDML KFING

References

[1]

"Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis." Martin F., Kohler A., Murat C., Balestrini R., Coutinho P.M., Jaillon O., Montanini B., Morin E., Noel B., Percudani R., Porcel B., Rubini A., Amicucci A., Amselem J., Anthouard V., Arcioni S., Artiguenave F., Aury J.M. , Ballario P., Bolchi A., Brenna A., Brun A., Buee M., Cantarel B., Chevalier G., Couloux A., Da Silva C., Denoeud F., Duplessis S., Ghignone S., Hilselberger B., Iotti M., Marcais B., Mello A., Miranda M., Pacioni G., Quesneville H., Riccioni C., Ruotolo R., Splivallo R., Stocchi V., Tisserant E., Viscomi A.R., Zambonelli A., Zampieri E., Henrissat B., Lebrun M.H., Paolocci F., Bonfante P., Ottonello S., Wincker P. Nature 464:1033-1038(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mel28.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN430320 Genomic DNA. Translation: CAZ84072.1.
RefSeq	XP_002839881.1. XM_002839835.1.
UniGene	Tme.2640.
3D structure databases
ProteinModelPortal	D5GHP2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CAZ84072; CAZ84072; GSTUM_00008071001.
GeneID	9183726.
KEGG	tml:GSTUM_00008071001.
Phylogenomic databases
KO	K14213.
Family and domain databases
Gene3D	3.90.230.10. 1 hit.
InterPro	IPR007865. Aminopep_P_N. IPR000994. Pept_M24_structural-domain. [Graphical view]
Pfam	PF05195. AMP_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SMART	SM01011. AMP_N. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
PROSITE	PS00491. PROLINE_PEPTIDASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPP2_TUBMM

Accession

Primary (citable) accession number: D5GHP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	June 15, 2010
Last modified:	March 6, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program