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D5GFR0

- MTAP_TUBMM

UniProt

D5GFR0 - MTAP_TUBMM

Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

GSTUM_00007013001

Organism
Tuber melanosporum (strain Mel28) (Perigord black truffle)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 2 (25 Jan 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

    Catalytic activityi

    S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei19 – 191PhosphateUniRule annotation
    Sitei183 – 1831Important for substrate specificityUniRule annotation
    Binding sitei201 – 2011Substrate; via amide nitrogenUniRule annotation
    Binding sitei202 – 2021PhosphateUniRule annotation
    Sitei238 – 2381Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    UniPathwayiUPA00904; UER00873.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
    Alternative name(s):
    5'-methylthioadenosine phosphorylaseUniRule annotation
    Short name:
    MTA phosphorylaseUniRule annotation
    Short name:
    MTAPUniRule annotation
    Short name:
    MTAPaseUniRule annotation
    Gene namesi
    ORF Names:GSTUM_00007013001
    OrganismiTuber melanosporum (strain Mel28) (Perigord black truffle)
    Taxonomic identifieri656061 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaPezizomycetesPezizalesTuberaceaeTuber
    ProteomesiUP000006911: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm UniRule annotation. Nucleus UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309S-methyl-5'-thioadenosine phosphorylasePRO_0000415136Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD5GFR0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni64 – 652Phosphate bindingUniRule annotation
    Regioni97 – 982Phosphate bindingUniRule annotation
    Regioni225 – 2273Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    OrthoDBiEOG77DJGM.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D5GFR0-1 [UniParc]FASTAAdd to Basket

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    MPLPDTFSES VRIAVIGGTG ISALEAEGFT AVASLDIQTP YGAPGSPLTI    50
    LKTPQNTPIA FLSRHGLHHE LTPTEIPNRA NIAALRSIGV RSIIAFSAVG 100
    SLQEHIRPRD FVVPSQIIDR TKGLRASTFF EGGLVGHVGF ADPFDSELSK 150
    VVFDIGSDGV LNGEGVKMHK DALLICMEGP QFSTRAESNL YRSWGGDVIN 200
    MSALPEAKLA KEAEIAYVMV CMSTDYDCWK TNEAAVTVET VMGNMHANGA 250
    NAKHLAAAIL KELAKEEHQD LVGAKHLEGL SKWACCTAPA GRKAEVVKKI 300
    EFMLPGYFS 309
    Length:309
    Mass (Da):33,148
    Last modified:January 25, 2012 - v2
    Checksum:i0D3FA92C97BF9093
    GO

    Sequence cautioni

    The sequence CAZ83353.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN430220 Genomic DNA. Translation: CAZ83353.1. Different initiation.
    UniGeneiTme.3343.

    Genome annotation databases

    EnsemblFungiiCAZ83353; CAZ83353; GSTUM_00007013001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN430220 Genomic DNA. Translation: CAZ83353.1 . Different initiation.
    UniGenei Tme.3343.

    3D structure databases

    ProteinModelPortali D5GFR0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CAZ83353 ; CAZ83353 ; GSTUM_00007013001 .

    Phylogenomic databases

    OrthoDBi EOG77DJGM.

    Enzyme and pathway databases

    UniPathwayi UPA00904 ; UER00873 .

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01694. MTAP. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mel28.

    Entry informationi

    Entry nameiMTAP_TUBMM
    AccessioniPrimary (citable) accession number: D5GFR0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 25, 2012
    Last modified: October 1, 2014
    This is version 27 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3