D5EVT3 (D5EVT3_PRER2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase HAMAP-Rule MF_00033
EC=2.4.1.227 HAMAP-Rule MF_00033

Alternative name(s):
Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase HAMAP-Rule MF_00033

Gene names

Name:	murG HAMAP-Rule MF_00033 EMBL ADE82097.1
Ordered Locus Names:	PRU_2332

Organism

Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23) [Complete proteome] [HAMAP]

Taxonomic identifier

264731 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Prevotellaceae › Prevotella › Prevotella ruminicola

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) By similarity. HAMAP-Rule MF_00033 SAAS SAAS006009
Catalytic activity	UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP-Rule MF_00033 SAAS SAAS006009
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00033 SAAS SAAS006009
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00033.
Sequence similarities	Belongs to the glycosyltransferase 28 family. MurG subfamily. HAMAP-Rule MF_00033

Ontologies

Keywords
Biological process	Cell cycle Cell division HAMAP-Rule MF_00033 SAAS SAAS006009 Cell shape Cell wall biogenesis/degradation HAMAP-Rule MF_00033 SAAS SAAS006009 Peptidoglycan synthesis HAMAP-Rule MF_00033 SAAS SAAS006009
Cellular component	Cell inner membrane HAMAP-Rule MF_00033 Cell membrane Membrane
Molecular function	Glycosyltransferase HAMAP-Rule MF_00033 SAAS SAAS006009 EMBL ADE82097.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: HAMAP peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	D5EVT3 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: 18C5D9F85B1626AC	FASTA	367	39,309
	10 20 30 40 50 60 MEELRVIISG GGTGGHIFPA VSIANAVKAL RPDAKILFVG ALGRMEMQRV PAAGYEIKGL 70 80 90 100 110 120 PICGFDRKNL LKNFKVLYKI WKSQRMAKQI IKDFKPQVAV GVGGYASGPT LNKAAAMGIP 130 140 150 160 170 180 CLIQEQNSYA GVTNKLLAKK AAKICVAYEG MERFFPADKI ILTGNPVRQA LLDTKISRED 190 200 210 220 230 240 AIKTFGLDPA KKTILLVGGS LGARTVNESV LQHLDLVKAA DAQFIWQTGK YYSAEIAKRL 250 260 270 280 290 300 KGQNIPNLVV TDFITDMGAA YKAADLVISR AGASSISEFC LIGKPVILVP SPNVAEDHQT 310 320 330 340 350 360 KNALALANRD AAIYVKDADA PATLLELAIK TVADAQKLQS LSENVLKLAL PDSADIIAKE VIKLAGK

References

[1]

"Comparative genome analysis of Prevotella ruminicola and Prevotella bryantii: insights into their environmental niche." the North American Consortium for Rumen Bacteria Purushe J., Fouts D.E., Morrison M., White B.A., Mackie R.I., Coutinho P.M., Henrissat B., Nelson K.E. Microb. Ecol. 60:721-729(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19189 / JCM 8958 / 23.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002006 Genomic DNA. Translation: ADE82097.1.
RefSeq	YP_003575588.1. NC_014033.1.
3D structure databases
ProteinModelPortal	D5EVT3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADE82097; ADE82097; PRU_2332.
GeneID	9001217.
KEGG	pru:PRU_2332.
PATRIC	35498801. VBIPreRum14515_2262.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000218323.
KO	K02563.
Enzyme and pathway databases
BioCyc	PRUM264731:GHX6-2260-MONOMER.
UniPathway	UPA00219.
Family and domain databases
HAMAP	MF_00033. MurG.
InterPro	IPR006009. GlcNAc_MurG. IPR004276. Glyco_trans_28. IPR007235. Glyco_trans_28_C. [Graphical view]
PANTHER	PTHR21015:SF22. PTHR21015:SF22. 1 hit.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF03033. Glyco_transf_28. 1 hit. [Graphical view]
TIGRFAMs	TIGR01133. murG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D5EVT3_PRER2

Accession

Primary (citable) accession number: D5EVT3

Entry history

Integrated into UniProtKB/TrEMBL:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	May 29, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)