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D5EGA4 (D5EGA4_AMICL) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Amico_1469 EMBL ADE57586.1
OrganismAminobacterium colombiense (strain DSM 12261 / ALA-1) [Complete proteome] [HAMAP] EMBL ADE57586.1
Taxonomic identifier572547 [NCBI]
Taxonomic lineageBacteriaSynergistetesSynergistiaSynergistalesSynergistaceaeAminobacterium

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain115 – 318204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding145 – 20056ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2721Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2851Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2851Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2871Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D5EGA4 [UniParc].

Last modified June 15, 2010. Version 1.
Checksum: 816B35BD8C6ABC9F

FASTA32835,474
        10         20         30         40         50         60 
MLGQRNEDDI VDKKLNVAVL YGGDGPEREV SLKSGAAVVS ALEEAGFKVV PFDITDLGQL 

        70         80         90        100        110        120 
SDLRKTGHID SVFIALHGSW GEDGRVQSAL DALRLPYSGS GSLACCLSMD KAATKGLLNL 

       130        140        150        160        170        180 
FGVPTPEGVL VLKGSSFSLE KARHFAERHH SVVVKPCNGG STLGVTVVHN SQGLSDAFNE 

       190        200        210        220        230        240 
AWKYDSRCVI EKYIDGVDLA VTVWKQKGYL EALPEVMICP EKGFYDYDSK YTPGKTRYIV 

       250        260        270        280        290        300 
PAPFDPSIRS DIHQMACRAF EALSCEGYAR VDFRVEEKGT PWVLEVNTAP GMTGTSLTPK 

       310        320 
AAAAAGYDFP AFLSHVVCLS LEKKSACR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001997 Genomic DNA. Translation: ADE57586.1.
RefSeqYP_003554310.1. NC_014011.1.

3D structure databases

ProteinModelPortalD5EGA4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE57586; ADE57586; Amico_1469.
GeneID8964421.
KEGGaco:Amico_1469.
PATRIC35455260. VBIAmiCol31354_1521.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.

Enzyme and pathway databases

BioCycACOL572547:GHQ0-1524-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD5EGA4_AMICL
AccessionPrimary (citable) accession number: D5EGA4
Entry history
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: February 19, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)