D5EEW5 (D5EEW5_AMICL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2 RuleBase RU000644 HAMAP-Rule MF_00100

Gene names

Name:	infB HAMAP-Rule MF_00100
Ordered Locus Names:	Amico_0972

Organism

Aminobacterium colombiense (strain DSM 12261 / ALA-1) [Complete proteome] [HAMAP] EMBL ADE57097.1

Taxonomic identifier

572547 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Synergistetes › Synergistia › Synergistales › Synergistaceae › Aminobacterium › Aminobacterium colombiense

Protein attributes

Sequence length	654 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00100 RuleBase RU000645.
Sequence similarities	Belongs to the IF-2 family. HAMAP-Rule MF_00100

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm HAMAP-Rule MF_00100
Ligand	GTP-binding HAMAP-Rule MF_00100 RuleBase RU000323 Nucleotide-binding
Molecular function	Initiation factor RuleBase RU000644 HAMAP-Rule MF_00100 EMBL ADE57097.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	162 – 169	8	GTP By similarity HAMAP-Rule MF_00100
	Nucleotide binding	208 – 212	5	GTP By similarity HAMAP-Rule MF_00100
	Nucleotide binding	262 – 265	4	GTP By similarity HAMAP-Rule MF_00100
	Region	156 – 304	149	G-domain By similarity HAMAP-Rule MF_00100

Sequences

	Sequence		Length	Mass (Da)
	D5EEW5 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: 31A7EF39D3768451	FASTA	654	71,926
	10 20 30 40 50 60 MSKIRVYELA KMLEISNKDL LTMLEDLGID AKTHMSSIDN DAAQLVEETI RKKEAKQPPK 70 80 90 100 110 120 TQKEETKVKE KQTIRVREAA TVKEVAQVID VPVAEAVKAL LDLGIMAPAG AQVDETILIA 130 140 150 160 170 180 LGEAYGIDFE YEEPEEEKVA TGRPVFHGKH MEPRPPIVTV MGHVDHGKTT LLDYIRQTNI 190 200 210 220 230 240 TAREAGGITQ HIGASTVTYE GKNIVFLDTP GHEAFTAMRA RGAQATDIAI LVVAADDGLM 250 260 270 280 290 300 PQTKEAINHA RAAGVPIVVA VNKIDKPAAR PDRVRQQLSD VGLVPEEWGG DSIMVDVSAK 310 320 330 340 350 360 TGEGIPHLLE MVLLVAEMEE LRADPTVTPE GIVIEANLDK GKGPVATVIV QQGTLRKGDI 370 380 390 400 410 420 ILLDTTWGKI RAMLDSRGRQ FLKAGPSTPV EILGLTSVPQ PGEHFVRVDN EKSARDEISK 430 440 450 460 470 480 REQEKRESEN VTAKRLTLEE LYSQLKEGEI PQLNLLVKTD VQGTNEALCA ALEKMGTNEV 490 500 510 520 530 540 GINIIHRGVG RIAESDIMLA TASNAVVIGF NVRPDKNAER IAEVEGVQIR LYNVIYDVVD 550 560 570 580 590 600 DVKAALEGML TPTIKEEELG QAEIRQVFKV PKAGKIAGCY VTEGMIKRNC RVRIIRDGVV 610 620 630 640 650 IWTGSLANLR RFKDEAKEVK AGYECGMSFT NYQDFREGDV VEAYELVEEK RYLE

References

[1]

"Complete genome sequence of Aminobacterium colombiense type strain (ALA-1)." Chertkov O., Sikorski J., Brambilla E., Lapidus A., Copeland A., Glavina Del Rio T., Nolan M., Lucas S., Tice H., Cheng J., Han C., Detter J., Bruce D., Tapia R., Goodwin L., Pitluck S., Liolios K., Ivanova N. , Mavromatis K., Ovchinnikova G., Pati A., Chen A., Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Spring S., Rohde M., Goker M., Bristow J., Eisen J., Markowitz V., Hugenholtz P., Kyrpides N., Klenk H. Stand. Genomic Sci. 2:280-289(2010) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 12261 / ALA-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001997 Genomic DNA. Translation: ADE57097.1.
RefSeq	YP_003553821.1. NC_014011.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADE57097; ADE57097; Amico_0972.
GeneID	8963915.
KEGG	aco:Amico_0972.
PATRIC	35454220. VBIAmiCol31354_1009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000076906.
KO	K02519.
OMA	HIGAFTV.
Enzyme and pathway databases
BioCyc	ACOL572547:GHQ0-1017-MONOMER.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
HAMAP	MF_00100_B. IF_2_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. PF04760. IF2_N. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit. SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D5EEW5_AMICL

Accession

Primary (citable) accession number: D5EEW5

Entry history

Integrated into UniProtKB/TrEMBL:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	May 29, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)