D5EBS7 (RIBL_METMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FAD synthase
EC=2.7.7.2

Alternative name(s):
FMN adenylyltransferase
Flavin adenine dinucleotide synthase

Gene names

Name:	ribL
Ordered Locus Names:	Mmah_1121

Organism

Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP) [Complete proteome] [HAMAP]

Taxonomic identifier

547558 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanohalophilus › Methanohalophilus mahii

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme By similarity. HAMAP-Rule MF_02115
Catalytic activity	ATP + FMN = diphosphate + FAD. HAMAP-Rule MF_02115
Cofactor	Divalent metal cations By similarity.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. HAMAP-Rule MF_02115
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the archaeal FAD synthase family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: HAMAP FMN metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP FMN adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 142	142	FAD synthase HAMAP-Rule MF_02115		PRO_0000406263
Regions
	Nucleotide binding	9 – 10	2	ATP By similarity
	Nucleotide binding	14 – 17	4	ATP By similarity
	Nucleotide binding	90 – 93	4	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	D5EBS7 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: D08690A95B8929B3	FASTA	142	16,484
	10 20 30 40 50 60 MTRILATGTF DILHPGHLYY LEQARKYGNE LYVLVARDST IEHKPKPIVP EKQRLEMVKA 70 80 90 100 110 120 LRVVDHALLG SEEDMFKPLK EVQPDIIVLG HDQVFDEKEL EDKLQKRGFN TKVVRLGKPH 130 140 QCTLCSSGRI IKRILERKRT EL

References

[1]

"The complete genome of Methanohalophilus mahii DSM 5219." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Lykidis A., Saunders E., Brettin T., Detter J.C., Han C., Land M. , Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Schneider S., Schroeder M., Klenk H.-P., Eisen J.A. Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35705 / DSM 5219 / SLP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001994 Genomic DNA. Translation: ADE36628.1.
RefSeq	YP_003542273.1. NC_014002.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADE36628; ADE36628; Mmah_1121.
GeneID	8983288.
KEGG	mmh:Mmah_1121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000284153.
KO	K14656.
OMA	VAHDETV.
Enzyme and pathway databases
BioCyc	MMAH547558:GHP5-1157-MONOMER.
UniPathway	UPA00277; UER00407.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_02115. FAD_synth_arch.
InterPro	IPR004821. Cyt_trans-like. IPR024902. FAD_synth_RibL. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF01467. CTP_transf_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBL_METMS

Accession

Primary (citable) accession number: D5EBS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program