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D5EBP8 (D5EBP8_METMS) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase HAMAP-Rule MF_00407
EC=6.5.1.1 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
Ordered Locus Names:Mmah_1091
OrganismMethanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP) [Complete proteome] [HAMAP] EMBL ADE36599.1
Taxonomic identifier547558 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanohalophilus ›

Protein attributes

Sequence length569 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). RuleBase RU000617 HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. RuleBase RU004196 HAMAP-Rule MF_00407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2471N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2451ATP By similarity HAMAP-Rule MF_00407
Binding site2521ATP By similarity HAMAP-Rule MF_00407
Binding site2671ATP By similarity HAMAP-Rule MF_00407
Binding site2971ATP By similarity HAMAP-Rule MF_00407
Binding site3351ATP By similarity HAMAP-Rule MF_00407
Binding site4121ATP By similarity HAMAP-Rule MF_00407
Binding site4181ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
D5EBP8 [UniParc].

Last modified June 15, 2010. Version 1.
Checksum: B657E9C1668A102C

FASTA56964,705
        10         20         30         40         50         60 
MASFRKLVEL YEELDNSTSH MKIIELLAEH FQSLEPAEVK ITAYLALGTI GPRFEKIDLG 

        70         80         90        100        110        120 
IGDKYGIQAI SRAYNVDKEK VEIAFGNQGD LGDVASALNE RQRSSLTLNY VFQHFVQIRD 

       130        140        150        160        170        180 
ISGKGSQKRK INLLAEVLSK ARPLESRYIM RIAQGNLRLG FGEKFLLEAL ALAFTGSRDN 

       190        200        210        220        230        240 
TSKVEDTYNI CTDIGQLGES LANHGMDSLG KFSIKIGRPV QMMLASRVDT MEELNEKFSG 

       250        260        270        280        290        300 
TMAAEEKYDG ERVQVHVDGD NIKAFSRRLT YISAQFPDVI EAARESVKAE KIVLDGEIVA 

       310        320        330        340        350        360 
YRQGEFRPFQ ELMQRRRKYH VEEYVKKIPV TVFFFDVLYL NGKSLMKWSY PERRQVLEEN 

       370        380        390        400        410        420 
LVETEKVKLA GRVVSADFSR IQHFFDDCVE KGMEGIIIKS VKAKSTYRPG KRGWLWVKWK 

       430        440        450        460        470        480 
KEYIEDVRET FDLVIVGSYY GKGRRKGSFG ALLCAVLNES EKRYETLAKV GTGFKDEDFE 

       490        500        510        520        530        540 
EINDLLKKYS IEKKPVNILI DSNMQPDNYI EPAVVIEVIG SEITSSPSHT SGQENSGNGY 

       550        560 
ALRFPRFLRI RYDKGPGDAT TVEEVKHLK

« Hide

References

[1]"The complete genome of Methanohalophilus mahii DSM 5219."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Lykidis A., Saunders E., Brettin T., Detter J.C., Han C., Land M. expand/collapse author list , Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Schneider S., Schroeder M., Klenk H.-P., Eisen J.A.
Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35705 / DSM 5219 / SLP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001994 Genomic DNA. Translation: ADE36599.1.
RefSeqYP_003542244.1. NC_014002.1.

3D structure databases

ProteinModelPortalD5EBP8.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE36599; ADE36599; Mmah_1091.
GeneID8983258.
KEGGmmh:Mmah_1091.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000036008.
KOK10747.
OMAIARRSHN.

Enzyme and pathway databases

BioCycMMAH547558:GHP5-1127-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD5EBP8_METMS
AccessionPrimary (citable) accession number: D5EBP8
Entry history
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info