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D5EB23 (D5EB23_METMS) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1381O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

Sequence LengthMass (Da)Tools
D5EB23 [UniParc].

Last modified June 15, 2010. Version 1.
Checksum: 02C9B22C9914B8D8

FASTA82492,330
        10         20         30         40         50         60 
MADYDDINPL DEKIEQHDGE GGRVVPVLIQ DEMKRSYIDY SMSVIVGRAL PDARDGLKPV 

        70         80         90        100        110        120 
HRRILHSMNE AGITHDKAYK KSARVVGDVL GKFHPHGDTA VYDSLVRMVQ DFSLRYPLID 

       130        140        150        160        170        180 
GQGNFGSVDG DSAAAMRYTE VRMDKITTEM LEDIKKGTVD FKPNYDGSLE EPEVLPSKLP 

       190        200        210        220        230        240 
NLLLNGSTGI AVGMATNMAP HNLGEVVDAT VKLIDDPETE LAELRKIVKG PDFPTGGIIM 

       250        260        270        280        290        300 
GTSGIKSAYE TGRSPIYLRA VTEIEEMKND KYRIVVYELP YQVNKARLVE SIANLVRDKK 

       310        320        330        340        350        360 
IEGISDLRDE SGREGMRVVI ELKKAANPKV LLNQLYKQTQ LETTFGIINL ALVDGVPRVL 

       370        380        390        400        410        420 
NLRELIQIYL KHRIDVISRR TVFDLRKAEE RAHILQGLKV ALDNIDEVIK LIRGSSTTEE 

       430        440        450        460        470        480 
AREGLISNFD LDEIQAKAIL DMKLQRLTGL ERQKIDEEYN GLIDTIADLK DILANDERKY 

       490        500        510        520        530        540 
AIIRDEVLEL KDKYGDERRT LIQSSREELA DEDLIPEEEV VVTSTQSGYI KNIPLQTYNM 

       550        560        570        580        590        600 
QHRGGRGVRG METKEDDTVG SIFVASTHDY ILFFTNRGKV YWQKVYDIPR GSRQSRGKAI 

       610        620        630        640        650        660 
VNLLELAEGE SVNAMIPVHD FENDRYLFMG TRDGTVKRCS LSDFSNPRKA GIIAISLKEG 

       670        680        690        700        710        720 
DELVNVLQTD GNQDIMLASR HGKALRISEE DVRVMGRTAR GVRGMKLAGP DVIVSLDVVD 

       730        740        750        760        770        780 
SDGTLLTVTE NGFGKRTPFD EYRTLRRGGQ GVITIFTDMR NGPVVTVKTV EDDDEIILTS 

       790        800        810        820 
SAGIVMRIPV TDIRVQGRNT KGVKIMNIRE KDKLVSLARI KKEE 

« Hide

References

[1]"The complete genome of Methanohalophilus mahii DSM 5219."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Lykidis A., Saunders E., Brettin T., Detter J.C., Han C., Land M. expand/collapse author list , Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Schneider S., Schroeder M., Klenk H.-P., Eisen J.A.
Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35705 / DSM 5219 / SLP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001994 Genomic DNA. Translation: ADE36374.1.
RefSeqYP_003542019.1. NC_014002.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE36374; ADE36374; Mmah_0851.
GeneID8983015.
KEGGmmh:Mmah_0851.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000076278.
KOK02469.
OMAETVDWVP.

Enzyme and pathway databases

BioCycMMAH547558:GHP5-885-MONOMER.

Family and domain databases

Gene3D3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPMF_01897. GyrA.
InterProIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD5EB23_METMS
AccessionPrimary (citable) accession number: D5EB23
Entry history
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: April 16, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)