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D5EA92 (D5EA92_METMS) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase RuleBase RU000617 HAMAP-Rule MF_00407

EC=6.5.1.1 RuleBase RU000617 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
Ordered Locus Names:Mmah_0567 EMBL ADE36093.1
OrganismMethanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP) [Complete proteome] [HAMAP] EMBL ADE36093.1
Taxonomic identifier547558 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanohalophilus

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). RuleBase RU000617 HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. HAMAP-Rule MF_00407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2561N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2541ATP By similarity HAMAP-Rule MF_00407
Binding site2611ATP By similarity HAMAP-Rule MF_00407
Binding site2761ATP By similarity HAMAP-Rule MF_00407
Binding site3061ATP By similarity HAMAP-Rule MF_00407
Binding site3461ATP By similarity HAMAP-Rule MF_00407
Binding site4211ATP By similarity HAMAP-Rule MF_00407
Binding site4271ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
D5EA92 [UniParc].

Last modified June 15, 2010. Version 1.
Checksum: 3F8B391E8D73AF83

FASTA56162,553
        10         20         30         40         50         60 
MTSFEYFANT CASIEHIPGS LEMTDVIAKL LKEVTIEELP IVTHFVMGSV FPAWSDRQMG 

        70         80         90        100        110        120 
VGNRLLYTAL SKSSGVSEEE IENIIRKTGD IGETAVQALS SNPAGQSTFS AFIEEKPGME 

       130        140        150        160        170        180 
IKEVYERFTH IADTAGKRSH GTKIKNLQYL FNSATPIEAR YLARLAIEQL RIGVGEGIVR 

       190        200        210        220        230        240 
DAISKAFETD VDVVERALML TNDLGLVAVA ACNGGNEEVQ KLDIQLNRPV KMMLAQVTSS 

       250        260        270        280        290        300 
IQSALNDLGE VAVEWKFDGA RVQIHKEGEN IHIFSRRLEN VTSSLPDVVK EVQENVNADT 

       310        320        330        340        350        360 
AILEGEAVAI GKDGKPRAFQ DILKRFRRKY DVEAIAKAIP LKLNLFDVLY LNGESLIDNP 

       370        380        390        400        410        420 
LRKRRELLFQ NVQSSDRILV DRQVITDNEE EIQEIYADAL RAGHEGIMIK KPDAPYSPGK 

       430        440        450        460        470        480 
RGKNWLKKKP LMETLDLVVI GAEWGYGRRA NLIGSYALGC YDPDTGKFPA IGKVATGITD 

       490        500        510        520        530        540 
EKLAELTTLF SDLVVYEAGR KIELKPEIVF EIAFEEIQKS PNYESGYALR FPRLVNVRDD 

       550        560 
KSPEEAESLE RIGEIYHSQR S 

« Hide

References

[1]"The complete genome of Methanohalophilus mahii DSM 5219."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Lykidis A., Saunders E., Brettin T., Detter J.C., Han C., Land M. expand/collapse author list , Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Schneider S., Schroeder M., Klenk H.-P., Eisen J.A.
Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35705 / DSM 5219 / SLP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001994 Genomic DNA. Translation: ADE36093.1.
RefSeqYP_003541738.1. NC_014002.1.

3D structure databases

ProteinModelPortalD5EA92.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE36093; ADE36093; Mmah_0567.
GeneID8982715.
KEGGmmh:Mmah_0567.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000036008.
KOK10747.
OMAGRPRPFQ.

Enzyme and pathway databases

BioCycMMAH547558:GHP5-584-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD5EA92_METMS
AccessionPrimary (citable) accession number: D5EA92
Entry history
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: February 19, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)