ID   D5DME4_PRIM3            Unreviewed;       483 AA.
AC   D5DME4;
DT   15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT   15-JUN-2010, sequence version 1.
DT   27-MAR-2024, entry version 62.
DE   SubName: Full=Alpha-amylase {ECO:0000313|EMBL:ADF42028.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:ADF42028.1};
GN   Name=amyL {ECO:0000313|EMBL:ADF42028.1};
GN   OrderedLocusNames=BMD_5229 {ECO:0000313|EMBL:ADF42028.1};
OS   Priestia megaterium (strain DSM 319 / IMG 1521) (Bacillus megaterium).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=592022 {ECO:0000313|EMBL:ADF42028.1, ECO:0000313|Proteomes:UP000002365};
RN   [1] {ECO:0000313|EMBL:ADF42028.1, ECO:0000313|Proteomes:UP000002365}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 319 / IMG 1521 {ECO:0000313|Proteomes:UP000002365};
RX   PubMed=21705586; DOI=10.1128/JB.00449-11;
RA   Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K.,
RA   Koenig S.S., Huot Creasy H., Rosovitz M.J., Riley D.R., Daugherty S.,
RA   Martin M., Elbourne L.D., Paulsen I., Biedendieck R., Braun C.,
RA   Grayburn S., Dhingra S., Lukyanchuk V., Ball B., Ul-Qamar R., Seibel J.,
RA   Bremer E., Jahn D., Ravel J., Vary P.S.;
RT   "Genome sequences of the biotechnologically important Bacillus megaterium
RT   strains QM B1551 and DSM319.";
RL   J. Bacteriol. 193:4199-4213(2011).
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR   EMBL; CP001982; ADF42028.1; -; Genomic_DNA.
DR   RefSeq; WP_013085538.1; NZ_CP120609.1.
DR   AlphaFoldDB; D5DME4; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   KEGG; bmd:BMD_5229; -.
DR   PATRIC; fig|592022.4.peg.5209; -.
DR   HOGENOM; CLU_024572_2_0_9; -.
DR   Proteomes; UP000002365; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR   Gene3D; 2.40.30.140; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR015237; Alpha-amylase_C_pro.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF09154; Alpha-amy_C_pro; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Glycosidase {ECO:0000313|EMBL:ADF42028.1};
KW   Hydrolase {ECO:0000313|EMBL:ADF42028.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}.
FT   DOMAIN          5..391
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   ACT_SITE        233
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   ACT_SITE        263
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   BINDING         104
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         196
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         204
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         237
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ   SEQUENCE   483 AA;  56090 MW;  872863FBA4031B1E CRC64;
     MERNHTIMQF FEWHVPADGE HWQRLKELAP QLKEQGIDSV WIPPVTKGVS SEDNGYGVYD
     LYDLGEFDQK GTVRTKYGTK QELHEAIDAC HNHGINVYVD IVMNHKAAAD EKETFHVIEV
     DPMNRTEEIS EPFEIEGWTK FTFEGRGDQY SSFKWNFNHF NGTDYDDKNG KEGVFRIAGE
     NKSWNENVDQ EFGNYDYLMF ANIDYNHPEV REEMIKWGKW LADTLQCDGY RLDAIKHINH
     DFIKEFAHEL SSSQEKPFYF VGEFWNPELT ACQEFLDVID YQIDLFDVSL HYKLHEASQQ
     GRDFDLTTIF DDTLVKTHPL NVVTFVDNHD SQPNESLESW VEDWFKQSAY ALILLREDGY
     PCVFYGDYFG IGGEHPIKGK EKDISALLHV RYDKAYGQQD DYFDHPNTIG WVRHGVEEFE
     KSGCAVVISN GEDGEKRMFV GEHRSGQTWI DFTNNREDQV VIEEDGYGQF PVNGGSVSVW
     AEA
//