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Protein

LexA repressor

Gene

lexA

Organism
Bacillus megaterium (strain DSM 319)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotation

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127For autocatalytic cleavage activityUniRule annotation1
Active sitei165For autocatalytic cleavage activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi27 – 47H-T-H motifUniRule annotationAdd BLAST21

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, HydrolaseUniRule annotationSAAS annotationImported, RepressorUniRule annotationSAAS annotation
Biological processDNA damage, DNA repairUniRule annotationSAAS annotation, DNA replicationUniRule annotationSAAS annotation, SOS responseUniRule annotationSAAS annotation, Transcription, Transcription regulationUniRule annotationSAAS annotation

Protein family/group databases

MEROPSiS24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressorUniRule annotationSAAS annotation (EC:3.4.21.88UniRule annotation)
Gene namesi
Name:lexAUniRule annotationImported
Ordered Locus Names:BMD_4077Imported
OrganismiBacillus megaterium (strain DSM 319)Imported
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002365 Componenti: Chromosome

PTM / Processingi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei91 – 92Cleavage; by autolysisUniRule annotation2

Keywords - PTMi

Autocatalytic cleavageUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD5DKR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 64LexA_DNA_bindInterPro annotationAdd BLAST63
Domaini122 – 186Peptidase_S24InterPro annotationAdd BLAST65

Sequence similaritiesi

Belongs to the peptidase S24 family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000232168.
KOiK01356.
OMAiRQMMQAM.
OrthoDBiPOG091H029U.

Family and domain databases

CDDicd00090. HTH_ARSR. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00015. LexA. 1 hit.
InterProiView protein in InterPro
IPR011991. ArsR-like_HTH.
IPR006200. LexA.
IPR036286. LexA/Signal_pep-like_sf.
IPR006199. LexA_DNA-bd_dom.
IPR006197. Peptidase_S24_LexA.
IPR015927. Peptidase_S24_S26A/B/C.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PfamiView protein in Pfam
PF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.

Sequencei

Sequence statusi: Complete.

D5DKR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSKRQQDI LDFIKHEVKL KGYPPSVREI GEAVGLASSS TVHGHLARLE
60 70 80 90 100
SKGLIRRDPT KPRAIEVLQL DEVNIPKSNV INVPVIGKVT AGLPITAVEN
110 120 130 140 150
VEEYFPLPDK FVSPDDHVFM LEIMGESMIE AGILDGDMVI VRQQQTANNG
160 170 180 190 200
DIVVAMTEDN EATVKRFFKE ENFVRLQPEN STMAPIILRH VTILGKVTGV

YRTIH
Length:205
Mass (Da):22,847
Last modified:June 15, 2010 - v1
Checksum:i189CA7BF44519873
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001982 Genomic DNA. Translation: ADF40907.1.
RefSeqiWP_013058778.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF40907; ADF40907; BMD_4077.
GeneIDi29910156.
KEGGibmd:BMD_4077.

Similar proteinsi

Entry informationi

Entry nameiD5DKR1_BACMD
AccessioniPrimary (citable) accession number: D5DKR1
Entry historyiIntegrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: October 25, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported