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Protein

LexA repressor

Gene

lexA

Organism
Bacillus megaterium (strain DSM 319)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotationSAAS annotation

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei91 – 922Cleavage; by autolysisUniRule annotation
Active sitei127 – 1271For autocatalytic cleavage activityUniRule annotation
Active sitei165 – 1651For autocatalytic cleavage activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi27 – 4721H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. serine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. DNA replication Source: UniProtKB-HAMAP
  3. negative regulation of transcription, DNA-templated Source: UniProtKB-HAMAP
  4. SOS response Source: UniProtKB-HAMAP
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation, RepressorUniRule annotationSAAS annotation

Keywords - Biological processi

DNA damage, DNA repair, DNA replicationUniRule annotationSAAS annotation, SOS responseUniRule annotationSAAS annotation, Transcription, Transcription regulationUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-4077-MONOMER.

Protein family/group databases

MEROPSiS24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressorUniRule annotation (EC:3.4.21.88UniRule annotation)
Gene namesi
Name:lexAUniRule annotationImported
Ordered Locus Names:BMD_4077Imported
OrganismiBacillus megaterium (strain DSM 319)Imported
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002365: Chromosome

PTM / Processingi

Keywords - PTMi

Autocatalytic cleavageUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD5DKR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000232168.
KOiK01356.
OMAiIAETGMP.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA.
InterProiIPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.

Sequencei

Sequence statusi: Complete.

D5DKR1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLSKRQQDI LDFIKHEVKL KGYPPSVREI GEAVGLASSS TVHGHLARLE
60 70 80 90 100
SKGLIRRDPT KPRAIEVLQL DEVNIPKSNV INVPVIGKVT AGLPITAVEN
110 120 130 140 150
VEEYFPLPDK FVSPDDHVFM LEIMGESMIE AGILDGDMVI VRQQQTANNG
160 170 180 190 200
DIVVAMTEDN EATVKRFFKE ENFVRLQPEN STMAPIILRH VTILGKVTGV

YRTIH
Length:205
Mass (Da):22,847
Last modified:June 15, 2010 - v1
Checksum:i189CA7BF44519873
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001982 Genomic DNA. Translation: ADF40907.1.
RefSeqiYP_003599257.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF40907; ADF40907; BMD_4077.
GeneIDi9119468.
KEGGibmd:BMD_4077.
PATRICi37258317. VBIBacMeg104484_4072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001982 Genomic DNA. Translation: ADF40907.1.
RefSeqiYP_003599257.1. NC_014103.1.

3D structure databases

ProteinModelPortaliD5DKR1.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS24.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF40907; ADF40907; BMD_4077.
GeneIDi9119468.
KEGGibmd:BMD_4077.
PATRICi37258317. VBIBacMeg104484_4072.

Phylogenomic databases

HOGENOMiHOG000232168.
KOiK01356.
OMAiIAETGMP.

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-4077-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA.
InterProiIPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
    Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 319Imported.

Entry informationi

Entry nameiD5DKR1_BACMD
AccessioniPrimary (citable) accession number: D5DKR1
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: February 4, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.