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D5DKR1

- D5DKR1_BACMD

UniProt

D5DKR1 - D5DKR1_BACMD

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Protein

LexA repressor

Gene
lexA, BMD_4077
Organism
Bacillus megaterium (strain DSM 319)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei91 – 922Cleavage; by autolysis By similarityUniRule annotation
Active sitei127 – 1271For autocatalytic cleavage activity By similarityUniRule annotation
Active sitei165 – 1651For autocatalytic cleavage activity By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi27 – 4721H-T-H motif By similarityUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. serine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. DNA replication Source: UniProtKB-HAMAP
  3. negative regulation of transcription, DNA-templated Source: UniProtKB-HAMAP
  4. SOS response Source: UniProtKB-HAMAP
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotationsImported, RepressorUniRule annotationSAAS annotations

Keywords - Biological processi

DNA damage, DNA repair, DNA replicationUniRule annotationSAAS annotations, SOS responseUniRule annotationSAAS annotations, Transcription, Transcription regulationUniRule annotationSAAS annotations

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-4077-MONOMER.

Protein family/group databases

MEROPSiS24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressorUniRule annotation (EC:3.4.21.88UniRule annotation)
Gene namesi
Name:lexAUniRule annotationImported
Ordered Locus Names:BMD_4077Imported
OrganismiBacillus megaterium (strain DSM 319)Imported
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002365: Chromosome

PTM / Processingi

Keywords - PTMi

Autocatalytic cleavageUniRule annotation

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliD5DKR1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000232168.
KOiK01356.
OMAiHVTILGK.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA.
InterProiIPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.

Sequencei

Sequence statusi: Complete.

D5DKR1-1 [UniParc]FASTAAdd to Basket

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MKLSKRQQDI LDFIKHEVKL KGYPPSVREI GEAVGLASSS TVHGHLARLE    50
SKGLIRRDPT KPRAIEVLQL DEVNIPKSNV INVPVIGKVT AGLPITAVEN 100
VEEYFPLPDK FVSPDDHVFM LEIMGESMIE AGILDGDMVI VRQQQTANNG 150
DIVVAMTEDN EATVKRFFKE ENFVRLQPEN STMAPIILRH VTILGKVTGV 200
YRTIH 205
Length:205
Mass (Da):22,847
Last modified:June 15, 2010 - v1
Checksum:i189CA7BF44519873
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001982 Genomic DNA. Translation: ADF40907.1.
RefSeqiYP_003599257.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF40907; ADF40907; BMD_4077.
GeneIDi9119468.
KEGGibmd:BMD_4077.
PATRICi37258317. VBIBacMeg104484_4072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001982 Genomic DNA. Translation: ADF40907.1 .
RefSeqi YP_003599257.1. NC_014103.1.

3D structure databases

ProteinModelPortali D5DKR1.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S24.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADF40907 ; ADF40907 ; BMD_4077 .
GeneIDi 9119468.
KEGGi bmd:BMD_4077.
PATRICi 37258317. VBIBacMeg104484_4072.

Phylogenomic databases

HOGENOMi HOG000232168.
KOi K01356.
OMAi HVTILGK.

Enzyme and pathway databases

BioCyci BMEG592022:GIVX-4077-MONOMER.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPi MF_00015. LexA.
InterProi IPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view ]
PRINTSi PR00726. LEXASERPTASE.
SUPFAMi SSF51306. SSF51306. 1 hit.
TIGRFAMsi TIGR00498. lexA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
    Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 319.

Entry informationi

Entry nameiD5DKR1_BACMD
AccessioniPrimary (citable) accession number: D5DKR1
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: June 11, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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