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D5DKR1

- D5DKR1_BACMD

UniProt

D5DKR1 - D5DKR1_BACMD

Protein

LexA repressor

Gene

lexA

Organism
Bacillus megaterium (strain DSM 319)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotationSAAS annotation

    Catalytic activityi

    Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei91 – 922Cleavage; by autolysisUniRule annotation
    Active sitei127 – 1271For autocatalytic cleavage activityUniRule annotation
    Active sitei165 – 1651For autocatalytic cleavage activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi27 – 4721H-T-H motifUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. serine-type endopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA repair Source: UniProtKB-HAMAP
    2. DNA replication Source: UniProtKB-HAMAP
    3. negative regulation of transcription, DNA-templated Source: UniProtKB-HAMAP
    4. SOS response Source: UniProtKB-HAMAP
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    HydrolaseUniRule annotationSAAS annotationImported, RepressorUniRule annotationSAAS annotation

    Keywords - Biological processi

    DNA damage, DNA repair, DNA replicationUniRule annotationSAAS annotation, SOS responseUniRule annotationSAAS annotation, Transcription, Transcription regulationUniRule annotationSAAS annotation

    Keywords - Ligandi

    DNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciBMEG592022:GIVX-4077-MONOMER.

    Protein family/group databases

    MEROPSiS24.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    LexA repressorUniRule annotation (EC:3.4.21.88UniRule annotation)
    Gene namesi
    Name:lexAUniRule annotationImported
    Ordered Locus Names:BMD_4077Imported
    OrganismiBacillus megaterium (strain DSM 319)Imported
    Taxonomic identifieri592022 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000002365: Chromosome

    PTM / Processingi

    Keywords - PTMi

    Autocatalytic cleavageUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD5DKR1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S24 family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000232168.
    KOiK01356.
    OMAiHVTILGK.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.10.109.10. 1 hit.
    HAMAPiMF_00015. LexA.
    InterProiIPR006200. LexA.
    IPR006199. LexA_DNA-bd_dom.
    IPR028360. Peptidase_S24/S26_b-rbn.
    IPR006197. Peptidase_S24_LexA.
    IPR019759. Peptidase_S24_S26.
    IPR015927. Peptidase_S24_S26A/B/C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01726. LexA_DNA_bind. 1 hit.
    PF00717. Peptidase_S24. 1 hit.
    [Graphical view]
    PRINTSiPR00726. LEXASERPTASE.
    SUPFAMiSSF51306. SSF51306. 1 hit.
    TIGRFAMsiTIGR00498. lexA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D5DKR1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSKRQQDI LDFIKHEVKL KGYPPSVREI GEAVGLASSS TVHGHLARLE    50
    SKGLIRRDPT KPRAIEVLQL DEVNIPKSNV INVPVIGKVT AGLPITAVEN 100
    VEEYFPLPDK FVSPDDHVFM LEIMGESMIE AGILDGDMVI VRQQQTANNG 150
    DIVVAMTEDN EATVKRFFKE ENFVRLQPEN STMAPIILRH VTILGKVTGV 200
    YRTIH 205
    Length:205
    Mass (Da):22,847
    Last modified:June 15, 2010 - v1
    Checksum:i189CA7BF44519873
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001982 Genomic DNA. Translation: ADF40907.1.
    RefSeqiYP_003599257.1. NC_014103.1.

    Genome annotation databases

    EnsemblBacteriaiADF40907; ADF40907; BMD_4077.
    GeneIDi9119468.
    KEGGibmd:BMD_4077.
    PATRICi37258317. VBIBacMeg104484_4072.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001982 Genomic DNA. Translation: ADF40907.1 .
    RefSeqi YP_003599257.1. NC_014103.1.

    3D structure databases

    ProteinModelPortali D5DKR1.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S24.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADF40907 ; ADF40907 ; BMD_4077 .
    GeneIDi 9119468.
    KEGGi bmd:BMD_4077.
    PATRICi 37258317. VBIBacMeg104484_4072.

    Phylogenomic databases

    HOGENOMi HOG000232168.
    KOi K01356.
    OMAi HVTILGK.

    Enzyme and pathway databases

    BioCyci BMEG592022:GIVX-4077-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.10.109.10. 1 hit.
    HAMAPi MF_00015. LexA.
    InterProi IPR006200. LexA.
    IPR006199. LexA_DNA-bd_dom.
    IPR028360. Peptidase_S24/S26_b-rbn.
    IPR006197. Peptidase_S24_LexA.
    IPR019759. Peptidase_S24_S26.
    IPR015927. Peptidase_S24_S26A/B/C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01726. LexA_DNA_bind. 1 hit.
    PF00717. Peptidase_S24. 1 hit.
    [Graphical view ]
    PRINTSi PR00726. LEXASERPTASE.
    SUPFAMi SSF51306. SSF51306. 1 hit.
    TIGRFAMsi TIGR00498. lexA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
      Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
      Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 319Imported.

    Entry informationi

    Entry nameiD5DKR1_BACMD
    AccessioniPrimary (citable) accession number: D5DKR1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 15, 2010
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3