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D5DEH5

- NPRM_BACMD

UniProt

D5DEH5 - NPRM_BACMD

Protein

Bacillolysin

Gene

nprM

Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    Extracellular zinc metalloprotease.By similarity

    Catalytic activityi

    Similar, but not identical, to that of thermolysin.

    Cofactori

    Binds 4 calcium ions per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi303 – 3031Calcium 1By similarity
    Metal bindingi305 – 3051Calcium 1By similarity
    Metal bindingi384 – 3841Calcium 2By similarity
    Metal bindingi388 – 3881Zinc; catalyticPROSITE-ProRule annotation
    Active sitei389 – 3891PROSITE-ProRule annotation
    Metal bindingi392 – 3921Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi412 – 4121Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi423 – 4231Calcium 2By similarity
    Metal bindingi423 – 4231Calcium 3By similarity
    Metal bindingi429 – 4291Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi431 – 4311Calcium 2By similarity
    Metal bindingi431 – 4311Calcium 3By similarity
    Metal bindingi433 – 4331Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi436 – 4361Calcium 2By similarity
    Metal bindingi436 – 4361Calcium 3By similarity
    Metal bindingi439 – 4391Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi440 – 4401Calcium 4By similarity
    Metal bindingi446 – 4461Calcium 4By similarity
    Active sitei477 – 4771Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBMEG592022:GIVX-2285-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacillolysin (EC:3.4.24.28)
    Alternative name(s):
    Neutral protease
    Gene namesi
    Name:nprM
    Ordered Locus Names:BMD_2285
    OrganismiBacillus megaterium (strain DSM 319)
    Taxonomic identifieri592022 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000002365: Chromosome

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 245221Activation peptideSequence AnalysisPRO_0000396525Add
    BLAST
    Chaini246 – 562317BacillolysinPRO_0000396526Add
    BLAST

    Keywords - PTMi

    Zymogen

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M4 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000247250.
    OMAiSKYNNAF.

    Family and domain databases

    Gene3Di3.10.170.10. 1 hit.
    InterProiIPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view]
    PfamiPF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view]
    PRINTSiPR00730. THERMOLYSIN.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    D5DEH5-1 [UniParc]FASTAAdd to Basket

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    MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI    50
    SGKLSGPSSQ KAQDVVFHYM NTNKDKYKLG NENAQNSFKV TEVVKDPVEQ 100
    ATVVRLQQVY NNIPVWGSTQ LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ 150
    KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL YVYQNGSDTT YAYVVNLNFL 200
    SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK PAAKPVTGTN 250
    AIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL 300
    WTDTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGAPLKSTV 350
    HYSSGYNNAF WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THAVTERSSN 400
    LIYQYESGAL NEAISDIFGT LVEYYDNRNP DWEIGEDIYT PGTSGDALRS 450
    MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS GIINKAAYLL ANGGTHYGVT 500
    VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD LYGSGSQEVI 550
    SVGKSFDAVG VQ 562
    Length:562
    Mass (Da):60,862
    Last modified:June 15, 2010 - v1
    Checksum:iBE1F088C0844F49A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75070 Genomic DNA. Translation: CAA52964.1.
    CP001982 Genomic DNA. Translation: ADF39133.1.
    PIRiI40227.
    RefSeqiWP_013083132.1. NC_014103.1.
    YP_003597483.1. NC_014103.1.

    Genome annotation databases

    EnsemblBacteriaiADF39133; ADF39133; BMD_2285.
    GeneIDi9117676.
    KEGGibmd:BMD_2285.
    PATRICi37254631. VBIBacMeg104484_2238.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75070 Genomic DNA. Translation: CAA52964.1 .
    CP001982 Genomic DNA. Translation: ADF39133.1 .
    PIRi I40227.
    RefSeqi WP_013083132.1. NC_014103.1.
    YP_003597483.1. NC_014103.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADF39133 ; ADF39133 ; BMD_2285 .
    GeneIDi 9117676.
    KEGGi bmd:BMD_2285.
    PATRICi 37254631. VBIBacMeg104484_2238.

    Phylogenomic databases

    HOGENOMi HOG000247250.
    OMAi SKYNNAF.

    Enzyme and pathway databases

    BioCyci BMEG592022:GIVX-2285-MONOMER.

    Family and domain databases

    Gene3Di 3.10.170.10. 1 hit.
    InterProi IPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view ]
    Pfami PF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00730. THERMOLYSIN.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319."
      Meinhardt F., Busskamp M., Wittchen K.D.
      Appl. Microbiol. Biotechnol. 41:344-351(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
      Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
      Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 319.

    Entry informationi

    Entry nameiNPRM_BACMD
    AccessioniPrimary (citable) accession number: D5DEH5
    Secondary accession number(s): Q00891
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3