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Protein

Bacillolysin

Gene

nprM

Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Extracellular zinc metalloprotease.By similarity

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 4 Ca2+ ions per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi303Calcium 1By similarity1
Metal bindingi305Calcium 1By similarity1
Metal bindingi384Calcium 2By similarity1
Metal bindingi388Zinc; catalyticBy similarity1
Active sitei389By similarity1
Metal bindingi392Zinc; catalyticBy similarity1
Metal bindingi412Zinc; catalyticBy similarity1
Metal bindingi423Calcium 2By similarity1
Metal bindingi423Calcium 3By similarity1
Metal bindingi429Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi431Calcium 2By similarity1
Metal bindingi431Calcium 3By similarity1
Metal bindingi433Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi436Calcium 2By similarity1
Metal bindingi436Calcium 3By similarity1
Metal bindingi439Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi440Calcium 4By similarity1
Metal bindingi446Calcium 4By similarity1
Active sitei477Proton donorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:nprM
Ordered Locus Names:BMD_2285
OrganismiBacillus megaterium (strain DSM 319)
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002365 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000039652525 – 245Activation peptideSequence analysisAdd BLAST221
ChainiPRO_0000396526246 – 562BacillolysinAdd BLAST317

Keywords - PTMi

Zymogen

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000247250.
KOiK01400.
OMAiWDYYRTT.
OrthoDBiPOG091H0APZ.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D5DEH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI
60 70 80 90 100
SGKLSGPSSQ KAQDVVFHYM NTNKDKYKLG NENAQNSFKV TEVVKDPVEQ
110 120 130 140 150
ATVVRLQQVY NNIPVWGSTQ LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ
160 170 180 190 200
KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL YVYQNGSDTT YAYVVNLNFL
210 220 230 240 250
SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK PAAKPVTGTN
260 270 280 290 300
AIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL
310 320 330 340 350
WTDTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGAPLKSTV
360 370 380 390 400
HYSSGYNNAF WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THAVTERSSN
410 420 430 440 450
LIYQYESGAL NEAISDIFGT LVEYYDNRNP DWEIGEDIYT PGTSGDALRS
460 470 480 490 500
MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS GIINKAAYLL ANGGTHYGVT
510 520 530 540 550
VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD LYGSGSQEVI
560
SVGKSFDAVG VQ
Length:562
Mass (Da):60,862
Last modified:June 15, 2010 - v1
Checksum:iBE1F088C0844F49A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75070 Genomic DNA. Translation: CAA52964.1.
CP001982 Genomic DNA. Translation: ADF39133.1.
PIRiI40227.
RefSeqiWP_013083132.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF39133; ADF39133; BMD_2285.
GeneIDi9117676.
KEGGibmd:BMD_2285.
PATRICi37254631. VBIBacMeg104484_2238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75070 Genomic DNA. Translation: CAA52964.1.
CP001982 Genomic DNA. Translation: ADF39133.1.
PIRiI40227.
RefSeqiWP_013083132.1. NC_014103.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF39133; ADF39133; BMD_2285.
GeneIDi9117676.
KEGGibmd:BMD_2285.
PATRICi37254631. VBIBacMeg104484_2238.

Phylogenomic databases

HOGENOMiHOG000247250.
KOiK01400.
OMAiWDYYRTT.
OrthoDBiPOG091H0APZ.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPRM_BACMD
AccessioniPrimary (citable) accession number: D5DEH5
Secondary accession number(s): Q00891
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: June 15, 2010
Last modified: November 30, 2016
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.