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Protein

Bacillolysin

Gene

nprM

Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Extracellular zinc metalloprotease.By similarity

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 4 Ca2+ ions per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi303Calcium 1By similarity1
Metal bindingi305Calcium 1By similarity1
Metal bindingi384Calcium 2By similarity1
Metal bindingi388Zinc; catalyticBy similarity1
Active sitei389By similarity1
Metal bindingi392Zinc; catalyticBy similarity1
Metal bindingi412Zinc; catalyticBy similarity1
Metal bindingi423Calcium 2By similarity1
Metal bindingi423Calcium 3By similarity1
Metal bindingi429Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi431Calcium 2By similarity1
Metal bindingi431Calcium 3By similarity1
Metal bindingi433Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi436Calcium 2By similarity1
Metal bindingi436Calcium 3By similarity1
Metal bindingi439Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi440Calcium 4By similarity1
Metal bindingi446Calcium 4By similarity1
Active sitei477Proton donorBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBMEG592022:G1GHR-2279-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:nprM
Ordered Locus Names:BMD_2285
OrganismiBacillus megaterium (strain DSM 319)
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002365 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000039652525 – 245Activation peptideSequence analysisAdd BLAST221
ChainiPRO_0000396526246 – 562BacillolysinAdd BLAST317

Keywords - PTMi

Zymogen

Structurei

3D structure databases

SMRiD5DEH5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000247250
KOiK01400
OMAiHKRTTLA
OrthoDBiPOG091H0APZ

Family and domain databases

CDDicd09597 M4_neutral_protease, 1 hit
Gene3Di1.10.390.10, 1 hit
InterProiView protein in InterPro
IPR011096 FTP_domain
IPR025711 PepSY
IPR023612 Peptidase_M4
IPR027268 Peptidase_M4/M1_CTD_sf
IPR001570 Peptidase_M4_C_domain
IPR013856 Peptidase_M4_domain
PfamiView protein in Pfam
PF07504 FTP, 1 hit
PF03413 PepSY, 1 hit
PF01447 Peptidase_M4, 1 hit
PF02868 Peptidase_M4_C, 1 hit
PRINTSiPR00730 THERMOLYSIN
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D5DEH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI
60 70 80 90 100
SGKLSGPSSQ KAQDVVFHYM NTNKDKYKLG NENAQNSFKV TEVVKDPVEQ
110 120 130 140 150
ATVVRLQQVY NNIPVWGSTQ LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ
160 170 180 190 200
KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL YVYQNGSDTT YAYVVNLNFL
210 220 230 240 250
SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK PAAKPVTGTN
260 270 280 290 300
AIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL
310 320 330 340 350
WTDTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGAPLKSTV
360 370 380 390 400
HYSSGYNNAF WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THAVTERSSN
410 420 430 440 450
LIYQYESGAL NEAISDIFGT LVEYYDNRNP DWEIGEDIYT PGTSGDALRS
460 470 480 490 500
MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS GIINKAAYLL ANGGTHYGVT
510 520 530 540 550
VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD LYGSGSQEVI
560
SVGKSFDAVG VQ
Length:562
Mass (Da):60,862
Last modified:June 15, 2010 - v1
Checksum:iBE1F088C0844F49A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75070 Genomic DNA Translation: CAA52964.1
CP001982 Genomic DNA Translation: ADF39133.1
PIRiI40227
RefSeqiWP_013083132.1, NC_014103.1

Genome annotation databases

EnsemblBacteriaiADF39133; ADF39133; BMD_2285
KEGGibmd:BMD_2285

Similar proteinsi

Entry informationi

Entry nameiNPRM_BACMD
AccessioniPrimary (citable) accession number: D5DEH5
Secondary accession number(s): Q00891
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: June 15, 2010
Last modified: March 28, 2018
This is version 41 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health