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D5DEH5

- NPRM_BACMD

UniProt

D5DEH5 - NPRM_BACMD

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Protein

Bacillolysin

Gene
nprM, BMD_2285
Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Extracellular zinc metalloprotease By similarity.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi303 – 3031Calcium 1 By similarity
Metal bindingi305 – 3051Calcium 1 By similarity
Metal bindingi384 – 3841Calcium 2 By similarity
Metal bindingi388 – 3881Zinc; catalytic By similarity
Active sitei389 – 3891 By similarity
Metal bindingi392 – 3921Zinc; catalytic By similarity
Metal bindingi412 – 4121Zinc; catalytic By similarity
Metal bindingi423 – 4231Calcium 2 By similarity
Metal bindingi423 – 4231Calcium 3 By similarity
Metal bindingi429 – 4291Calcium 3; via carbonyl oxygen By similarity
Metal bindingi431 – 4311Calcium 2 By similarity
Metal bindingi431 – 4311Calcium 3 By similarity
Metal bindingi433 – 4331Calcium 2; via carbonyl oxygen By similarity
Metal bindingi436 – 4361Calcium 2 By similarity
Metal bindingi436 – 4361Calcium 3 By similarity
Metal bindingi439 – 4391Calcium 4; via carbonyl oxygen By similarity
Metal bindingi440 – 4401Calcium 4 By similarity
Metal bindingi446 – 4461Calcium 4 By similarity
Active sitei477 – 4771Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-2285-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:nprM
Ordered Locus Names:BMD_2285
OrganismiBacillus megaterium (strain DSM 319)
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002365: Chromosome

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Propeptidei25 – 245221Activation peptide Reviewed predictionPRO_0000396525Add
BLAST
Chaini246 – 562317BacillolysinPRO_0000396526Add
BLAST

Keywords - PTMi

Zymogen

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000247250.
OMAiSKYNNAF.

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D5DEH5-1 [UniParc]FASTAAdd to Basket

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MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI    50
SGKLSGPSSQ KAQDVVFHYM NTNKDKYKLG NENAQNSFKV TEVVKDPVEQ 100
ATVVRLQQVY NNIPVWGSTQ LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ 150
KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL YVYQNGSDTT YAYVVNLNFL 200
SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK PAAKPVTGTN 250
AIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL 300
WTDTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGAPLKSTV 350
HYSSGYNNAF WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THAVTERSSN 400
LIYQYESGAL NEAISDIFGT LVEYYDNRNP DWEIGEDIYT PGTSGDALRS 450
MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS GIINKAAYLL ANGGTHYGVT 500
VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD LYGSGSQEVI 550
SVGKSFDAVG VQ 562
Length:562
Mass (Da):60,862
Last modified:June 15, 2010 - v1
Checksum:iBE1F088C0844F49A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75070 Genomic DNA. Translation: CAA52964.1.
CP001982 Genomic DNA. Translation: ADF39133.1.
PIRiI40227.
RefSeqiWP_013083132.1. NC_014103.1.
YP_003597483.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF39133; ADF39133; BMD_2285.
GeneIDi9117676.
KEGGibmd:BMD_2285.
PATRICi37254631. VBIBacMeg104484_2238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75070 Genomic DNA. Translation: CAA52964.1 .
CP001982 Genomic DNA. Translation: ADF39133.1 .
PIRi I40227.
RefSeqi WP_013083132.1. NC_014103.1.
YP_003597483.1. NC_014103.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADF39133 ; ADF39133 ; BMD_2285 .
GeneIDi 9117676.
KEGGi bmd:BMD_2285.
PATRICi 37254631. VBIBacMeg104484_2238.

Phylogenomic databases

HOGENOMi HOG000247250.
OMAi SKYNNAF.

Enzyme and pathway databases

BioCyci BMEG592022:GIVX-2285-MONOMER.

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319."
    Meinhardt F., Busskamp M., Wittchen K.D.
    Appl. Microbiol. Biotechnol. 41:344-351(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
    Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 319.

Entry informationi

Entry nameiNPRM_BACMD
AccessioniPrimary (citable) accession number: D5DEH5
Secondary accession number(s): Q00891
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: June 15, 2010
Last modified: September 3, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi