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Protein

Bacillolysin

Gene

nprM

Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Extracellular zinc metalloprotease.By similarity

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 4 Ca2+ ions per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi303 – 3031Calcium 1By similarity
Metal bindingi305 – 3051Calcium 1By similarity
Metal bindingi384 – 3841Calcium 2By similarity
Metal bindingi388 – 3881Zinc; catalyticPROSITE-ProRule annotation
Active sitei389 – 3891PROSITE-ProRule annotation
Metal bindingi392 – 3921Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi412 – 4121Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi423 – 4231Calcium 2By similarity
Metal bindingi423 – 4231Calcium 3By similarity
Metal bindingi429 – 4291Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi431 – 4311Calcium 2By similarity
Metal bindingi431 – 4311Calcium 3By similarity
Metal bindingi433 – 4331Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi436 – 4361Calcium 2By similarity
Metal bindingi436 – 4361Calcium 3By similarity
Metal bindingi439 – 4391Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi440 – 4401Calcium 4By similarity
Metal bindingi446 – 4461Calcium 4By similarity
Active sitei477 – 4771Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-2285-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:nprM
Ordered Locus Names:BMD_2285
OrganismiBacillus megaterium (strain DSM 319)
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002365: Chromosome

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 245221Activation peptideSequence AnalysisPRO_0000396525Add
BLAST
Chaini246 – 562317BacillolysinPRO_0000396526Add
BLAST

Keywords - PTMi

Zymogen

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000247250.
OMAiSKYNNAF.

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D5DEH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKKQALKV LLSVGILSSS FAFAHTSSAA PNNVLSTEKY NKEIKSPEFI
60 70 80 90 100
SGKLSGPSSQ KAQDVVFHYM NTNKDKYKLG NENAQNSFKV TEVVKDPVEQ
110 120 130 140 150
ATVVRLQQVY NNIPVWGSTQ LAHVAKDGTL KVVSGTVAPD LDKKEKLKGQ
160 170 180 190 200
KQVDSKKAIQ AAEKDLGFKP TYEKSPSSEL YVYQNGSDTT YAYVVNLNFL
210 220 230 240 250
SPEPGNYYYF VDAISGKVLD KYNTIDSVAG PKADVKQAAK PAAKPVTGTN
260 270 280 290 300
AIGSGKGVLG DTKSLKTTLS SSTYYLQDNT RGATIYTYDA KNRTSLPGTL
310 320 330 340 350
WTDTDNTYNA TRDAAAVDAH YYAGVTYDYY KNKFNRNSYD NAGAPLKSTV
360 370 380 390 400
HYSSGYNNAF WNGSQMVYGD GDGTTFVPLS GGLDVIGHEL THAVTERSSN
410 420 430 440 450
LIYQYESGAL NEAISDIFGT LVEYYDNRNP DWEIGEDIYT PGTSGDALRS
460 470 480 490 500
MSNPAKYGDP DHYSKRYTGS SDNGGVHTNS GIINKAAYLL ANGGTHYGVT
510 520 530 540 550
VTGIGGDKLG KIYYRANTLY FTQSTTFSQA RAGLVQAAAD LYGSGSQEVI
560
SVGKSFDAVG VQ
Length:562
Mass (Da):60,862
Last modified:June 15, 2010 - v1
Checksum:iBE1F088C0844F49A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75070 Genomic DNA. Translation: CAA52964.1.
CP001982 Genomic DNA. Translation: ADF39133.1.
PIRiI40227.
RefSeqiWP_013083132.1. NC_014103.1.
YP_003597483.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF39133; ADF39133; BMD_2285.
GeneIDi9117676.
KEGGibmd:BMD_2285.
PATRICi37254631. VBIBacMeg104484_2238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75070 Genomic DNA. Translation: CAA52964.1.
CP001982 Genomic DNA. Translation: ADF39133.1.
PIRiI40227.
RefSeqiWP_013083132.1. NC_014103.1.
YP_003597483.1. NC_014103.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF39133; ADF39133; BMD_2285.
GeneIDi9117676.
KEGGibmd:BMD_2285.
PATRICi37254631. VBIBacMeg104484_2238.

Phylogenomic databases

HOGENOMiHOG000247250.
OMAiSKYNNAF.

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-2285-MONOMER.

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319."
    Meinhardt F., Busskamp M., Wittchen K.D.
    Appl. Microbiol. Biotechnol. 41:344-351(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
    Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 319.

Entry informationi

Entry nameiNPRM_BACMD
AccessioniPrimary (citable) accession number: D5DEH5
Secondary accession number(s): Q00891
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: June 15, 2010
Last modified: January 7, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.