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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Bacillus megaterium (strain DSM 319)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei195 – 1951ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-705-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.2UniRule annotationSAAS annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:trpSUniRule annotationImported
Ordered Locus Names:BMD_0705Imported
OrganismiBacillus megaterium (strain DSM 319)Imported
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002365: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD5DAJ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 189"HIGH" regionUniRule annotation
Motifi192 – 1965"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000059940.
KOiK01867.
OMAiGWGQFKP.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5DAJ0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTIFSGIQP SGTITLGNYI GAMKQFVELQ HDYNCHFCIV DQHAITAPQD
60 70 80 90 100
RLALRKNIRS LAALYVAVGI DPAKSTLFIQ SEVPAHTEAG WMLQCVAYIG
110 120 130 140 150
ELERMTQFKD KSAGKEAVSA GLLTYPPLMA ADILLYNTDL VPVGEDQKQH
160 170 180 190 200
LELTRDLAER FNKKYNDIFT IPEVRIPKVG ARVMSLQDPT KKMSKSDPNQ
210 220 230 240 250
KAFITLLDDA KTIEKKIKSA VTDSEGIVRY DKENKPGISN LLSIYSILAN
260 270 280 290 300
KSIEDIEAMY EGKGYGDFKA DTAKVVAEAL APIQERYYEL VDSSELDDIL
310 320
DQGADKANAT ANKMLRKMRN AMGIGRKR
Length:328
Mass (Da):36,501
Last modified:June 15, 2010 - v1
Checksum:iFD78423C2D9A2F69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001982 Genomic DNA. Translation: ADF37569.1.
RefSeqiYP_003595919.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF37569; ADF37569; BMD_0705.
GeneIDi9116096.
KEGGibmd:BMD_0705.
PATRICi37251379. VBIBacMeg104484_0617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001982 Genomic DNA. Translation: ADF37569.1.
RefSeqiYP_003595919.1. NC_014103.1.

3D structure databases

ProteinModelPortaliD5DAJ0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF37569; ADF37569; BMD_0705.
GeneIDi9116096.
KEGGibmd:BMD_0705.
PATRICi37251379. VBIBacMeg104484_0617.

Phylogenomic databases

HOGENOMiHOG000059940.
KOiK01867.
OMAiGWGQFKP.

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-705-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
    Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 319Imported.

Entry informationi

Entry nameiD5DAJ0_BACMD
AccessioniPrimary (citable) accession number: D5DAJ0
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: February 4, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.