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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Bacillus megaterium (strain DSM 319)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132L-tryptophanUniRule annotation1
Binding sitei183ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 11ATPUniRule annotation3
Nucleotide bindingi17 – 18ATPUniRule annotation2
Nucleotide bindingi144 – 146ATPUniRule annotation3
Nucleotide bindingi192 – 196ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotationImported
Ordered Locus Names:BMD_0705Imported
OrganismiBacillus megaterium (strain DSM 319)Imported
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002365 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD5DAJ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 18"HIGH" regionUniRule annotation9
Motifi192 – 196"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK
OrthoDBiPOG091H00I9

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

D5DAJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIFSGIQP SGTITLGNYI GAMKQFVELQ HDYNCHFCIV DQHAITAPQD
60 70 80 90 100
RLALRKNIRS LAALYVAVGI DPAKSTLFIQ SEVPAHTEAG WMLQCVAYIG
110 120 130 140 150
ELERMTQFKD KSAGKEAVSA GLLTYPPLMA ADILLYNTDL VPVGEDQKQH
160 170 180 190 200
LELTRDLAER FNKKYNDIFT IPEVRIPKVG ARVMSLQDPT KKMSKSDPNQ
210 220 230 240 250
KAFITLLDDA KTIEKKIKSA VTDSEGIVRY DKENKPGISN LLSIYSILAN
260 270 280 290 300
KSIEDIEAMY EGKGYGDFKA DTAKVVAEAL APIQERYYEL VDSSELDDIL
310 320
DQGADKANAT ANKMLRKMRN AMGIGRKR
Length:328
Mass (Da):36,501
Last modified:June 15, 2010 - v1
Checksum:iFD78423C2D9A2F69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001982 Genomic DNA Translation: ADF37569.1

Genome annotation databases

EnsemblBacteriaiADF37569; ADF37569; BMD_0705
KEGGibmd:BMD_0705

Similar proteinsi

Entry informationi

Entry nameiD5DAJ0_BACMD
AccessioniPrimary (citable) accession number: D5DAJ0
Entry historyiIntegrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: March 28, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health