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Protein
Submitted name:

Alpha-amylase, cytoplasmic

Gene

amyA

Organism
Escherichia coli O18:K1:H7 (strain IHE3034 / ExPEC)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Protein predictedi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei235 – 2351NucleophileUniRule annotation
Active sitei265 – 2651Proton donorUniRule annotation

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseImported, Hydrolase

Enzyme and pathway databases

BioCyciECOL714962:GI9T-2045-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha-amylase, cytoplasmicImported (EC:3.2.1.1Imported)
Gene namesi
Name:amyAImported
Ordered Locus Names:ECOK1_2045Imported
OrganismiEscherichia coli O18:K1:H7 (strain IHE3034 / ExPEC)Imported
Taxonomic identifieri714962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000002364: Chromosome

Structurei

3D structure databases

ProteinModelPortaliD5D4V4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

D5D4V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG
60 70 80 90 100
GYSVGYDSYD LFDLGEFDQK GCIPTKYGDK AQLLAAIDAL KRNDIAVLLD
110 120 130 140 150
VVVNHKMGAD EKEAIRVQRV NADDRTQIDE EIIECEGWTR YTFPARAGQY
160 170 180 190 200
SQFIWDFKCF SGIDHIEHPD EDGIFKIVND YTGEGWNDQV DDELGNFDYL
210 220 230 240 250
MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI PAWFYKEWIE
260 270 280 290 300
HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS
310 320 330 340 350
RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL
360 370 380 390 400
AYALILLREN GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL
410 420 430 440 450
ARQRFAHGVQ TLFFDHPNCI AFSRSGTDEY PGCVVVMSNG DDGEKTIHLG
460 470 480 490
ENYGNKTWRD FLGNRQESVV TDENGEATFF CNGGSVSVWV IEEVI
Length:495
Mass (Da):56,625
Last modified:June 15, 2010 - v1
Checksum:i3D86F6FD3217DA34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001969 Genomic DNA. Translation: ADE90034.1.
RefSeqiYP_006101225.1. NC_017628.1.

Genome annotation databases

EnsemblBacteriaiADE90034; ADE90034; ECOK1_2045.
GeneIDi12690613.
KEGGieih:ECOK1_2045.
PATRICi36705514. VBIEscCol148715_2083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001969 Genomic DNA. Translation: ADE90034.1.
RefSeqiYP_006101225.1. NC_017628.1.

3D structure databases

ProteinModelPortaliD5D4V4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE90034; ADE90034; ECOK1_2045.
GeneIDi12690613.
KEGGieih:ECOK1_2045.
PATRICi36705514. VBIEscCol148715_2083.

Phylogenomic databases

HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.

Enzyme and pathway databases

BioCyciECOL714962:GI9T-2045-MONOMER.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IHE3034 / ExPECImported.
  2. "Identification of protective and broadly conserved vaccine antigens from the genome of Extraintestinal Pathogenic Escherichia coli (ExPEC)."
    Moriel D.G., Bertoldi I., Spagnuolo A., Marchi S., Rosini R., Nesta B., Pastorello I., Mariani Corea V.A., Torricelli G., Cartocci E., Savino S., Scarselli M., Dobrindt U., Hacker J., Tettelin H., Wieler L.H., Ewers C., Picard D.
    , Dougan G., Fontana M.R., Rappuoli R., Pizza M., Serino L.
    Proc. Natl. Acad. Sci. U.S.A. 0:0-0(2010)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: IHE3034.

Entry informationi

Entry nameiD5D4V4_ECOKI
AccessioniPrimary (citable) accession number: D5D4V4
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: February 4, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.