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Protein
Submitted name:

Cytoplasmic alpha-amylase

Gene

ECL_01353

Organism
Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Protein predictedi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei235 – 2351NucleophileUniRule annotation
Active sitei265 – 2651Proton donorUniRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Enzyme and pathway databases

BioCyciECLO716541:GH13-1380-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
Cytoplasmic alpha-amylaseImported
Gene namesi
Ordered Locus Names:ECL_01353Imported
OrganismiEnterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)Imported
Taxonomic identifieri716541 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
ProteomesiUP000002363 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliD5CJR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

D5CJR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNPTLLQCF HWYYPAGGEL WREVTALAPN LNEIGINMIW LPPAYKGASG
60 70 80 90 100
GYSVGYDSYD LFDLGEFDQK DSVATKYGDK AQLLEAIDAL KSNNIAVLLD
110 120 130 140 150
VVVNHKMGAD EKEHVRVQRV NEQDRTQIDD EIIECEAWTR YTFPARAGQY
160 170 180 190 200
SQFVWDYKCF SGIDHIENPD EDGIFKIVND YTGEGWNDQV DDEMGNFDYL
210 220 230 240 250
MGENIDFRNH AVTEEIKYWA RWVMEQTGCD GFRLDAVKHI PAWFYKEWIE
260 270 280 290 300
HVQEVATQPL FIVAEYWSHE VDKLQAYINQ VEGKTMLFDA PLQMKFHEAS
310 320 330 340 350
RQGRDYDMSQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEAWFKPL
360 370 380 390 400
AYALILLREN GVPSVFYPDL FGASYDDTGG DGETYHIDMP VIEQLHELIL
410 420 430 440 450
ARQRFAHGVQ TLFFDHPNCI AFSRSGTEED PGCVVVMSNG DDGEKVICLG
460 470 480 490
ENYGNKTWRD FLGNREETVT TAADGEGTFT CKGGSVSVWV IEDAL
Length:495
Mass (Da):56,245
Last modified:June 14, 2010 - v1
Checksum:i322D90673B092590
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001918 Genomic DNA. Translation: ADF60914.1.
RefSeqiYP_003611863.1. NC_014121.1.

Genome annotation databases

EnsemblBacteriaiADF60914; ADF60914; ECL_01353.
GeneIDi9123857.
KEGGienc:ECL_01353.
PATRICi38453711. VBIEntClo148801_1600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001918 Genomic DNA. Translation: ADF60914.1.
RefSeqiYP_003611863.1. NC_014121.1.

3D structure databases

ProteinModelPortaliD5CJR7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF60914; ADF60914; ECL_01353.
GeneIDi9123857.
KEGGienc:ECL_01353.
PATRICi38453711. VBIEntClo148801_1600.

Phylogenomic databases

HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.

Enzyme and pathway databases

BioCyciECLO716541:GH13-1380-MONOMER.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Enterobacter cloacae subsp. cloacae type strain ATCC 13047."
    Ren Y., Ren Y., Zhou Z., Guo X., Li Y., Feng L., Wang L.
    J. Bacteriol. 192:2463-2464(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56Imported.

Entry informationi

Entry nameiD5CJR7_ENTCC
AccessioniPrimary (citable) accession number: D5CJR7
Entry historyi
Integrated into UniProtKB/TrEMBL: June 14, 2010
Last sequence update: June 14, 2010
Last modified: March 3, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.