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Protein

Beta-galactosidase

Gene

lacZ

Organism
Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011SubstrateUniRule annotation
Metal bindingi200 – 2001SodiumUniRule annotation
Binding sitei200 – 2001SubstrateUniRule annotation
Sitei356 – 3561Transition state stabilizerUniRule annotation
Sitei390 – 3901Transition state stabilizerUniRule annotation
Metal bindingi415 – 4151Magnesium 1UniRule annotation
Metal bindingi417 – 4171Magnesium 1UniRule annotation
Active sitei460 – 4601Proton donorUniRule annotation
Metal bindingi460 – 4601Magnesium 1UniRule annotation
Binding sitei460 – 4601SubstrateUniRule annotation
Active sitei536 – 5361NucleophileUniRule annotation
Metal bindingi596 – 5961Magnesium 2UniRule annotation
Metal bindingi600 – 6001Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi603 – 6031SodiumUniRule annotation
Binding sitei603 – 6031SubstrateUniRule annotation
Binding sitei998 – 9981SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationSAAS annotation, Hydrolase

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, SodiumUniRule annotation

Enzyme and pathway databases

BioCyciECLO716541:GH13-3731-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotationSAAS annotation (EC:3.2.1.23UniRule annotationSAAS annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:ECL_03691Imported
OrganismiEnterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)Imported
Taxonomic identifieri716541 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
ProteomesiUP000002363 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD5C6R3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 5394Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252443.
KOiK01190.
OMAiTNRHEHH.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5C6R3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITDSLAVVL QRRDWENPGV TQLNRLAAHP PFASWRNSEE ARTDRPSQES
60 70 80 90 100
RSLNGEWRFA WFPAPEAVPE SWLERDLPDA DTVIVPSNWQ MHGYDAPIYT
110 120 130 140 150
NVTYPIAVNP PYVPTENPTG CYSLTFNVDE SWLQEGQTRI IFDGVNSAFH
160 170 180 190 200
LWCNGRWVGY GQDSRLPSEF DLSAFLHAGE NRLAVMVLRW SDGSYLEDQD
210 220 230 240 250
MWRMSGIFRD VSLLHKPSTQ ISDFHVATHF NDDFSRAVLE ADVQMYGELR
260 270 280 290 300
DELRVTVSLW QGETQVASGT APFGGEIIDE RGGYADHVTL RLNVENPKLW
310 320 330 340 350
SAEIPNLYRA VVELHTADGT LIEAEACDVG FREVRIENGL LLLNGKPLLI
360 370 380 390 400
RGVNRHEHHP LHGQVMDEQT MVQDILLMKQ NNFNAVRCSH YPNHPLWYTL
410 420 430 440 450
CDHYGLYVVD EANIETHGMV PMNRLTDDPR WLPAMSERVT RMVQRDRNHP
460 470 480 490 500
SVIIWSLGNE SGHGANHDAL YRWIKSVDPS RPVQYEGGGA DTFATDIICP
510 520 530 540 550
MYARVDEDQP FPAVPKWSIK KWLSLPGETR PLILCEYAHA MGNSLGGFAK
560 570 580 590 600
YWQAFRQYPR LQGGFVWDWV DQSLIKYDEN GNPWSAYGGD FGDKPNDRQF
610 620 630 640 650
CMNGLVFADR TPHPALTEAK HQQQFFQFSL SGRTIEVTSE YLFRHSDNEL
660 670 680 690 700
LHWMVALDGK PLASGEVPLD VAPQGKQLIE LPELPQPKSA GQLWLTVHVV
710 720 730 740 750
QPNATTWSAA GHISAWQQWR LAENLSVTLP SAPHAIPQLT TSETDFCIEL
760 770 780 790 800
DNKRWQFNRQ SGFLSQMWIG DKKQLLTPLR DQFTRAPLDN DIGVSEATRI
810 820 830 840 850
DPNAWVERWK AAGHYQAEAA LLQCTADTLA DAVLITTVHA WQHQGKTLFI
860 870 880 890 900
SRKTYRIDGS GQMAITVDVE VASNTPHPAR IGLTCQLAQV AERVNWLGLG
910 920 930 940 950
PQENYPDRLT AACFDRWDLP LSDMYTPYVF PSENGLRCGT RELNYGPHQW
960 970 980 990 1000
RGDFQFNISR YSQQQLMETS HRHLLHAEEG TWLNIDGFHM GIGGDDSWSP
1010 1020
SVSAEFHLSA GSYHYQLLWC QK
Length:1,022
Mass (Da):116,183
Last modified:June 15, 2010 - v1
Checksum:i1B8314BA008F69EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001918 Genomic DNA. Translation: ADF63225.1.
RefSeqiWP_013098161.1. NC_014121.1.
YP_003614174.1. NC_014121.1.

Genome annotation databases

EnsemblBacteriaiADF63225; ADF63225; ECL_03691.
GeneIDi9126168.
KEGGienc:ECL_03691.
PATRICi38458276. VBIEntClo148801_3849.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001918 Genomic DNA. Translation: ADF63225.1.
RefSeqiWP_013098161.1. NC_014121.1.
YP_003614174.1. NC_014121.1.

3D structure databases

ProteinModelPortaliD5C6R3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF63225; ADF63225; ECL_03691.
GeneIDi9126168.
KEGGienc:ECL_03691.
PATRICi38458276. VBIEntClo148801_3849.

Phylogenomic databases

HOGENOMiHOG000252443.
KOiK01190.
OMAiTNRHEHH.

Enzyme and pathway databases

BioCyciECLO716541:GH13-3731-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Enterobacter cloacae subsp. cloacae type strain ATCC 13047."
    Ren Y., Ren Y., Zhou Z., Guo X., Li Y., Feng L., Wang L.
    J. Bacteriol. 192:2463-2464(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56Imported.

Entry informationi

Entry nameiD5C6R3_ENTCC
AccessioniPrimary (citable) accession number: D5C6R3
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: April 1, 2015
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.