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D5C6R3

- D5C6R3_ENTCC

UniProt

D5C6R3 - D5C6R3_ENTCC

Protein

Beta-galactosidase

Gene

lacZ

Organism
Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 28 (01 Oct 2014)
      Sequence version 1 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 sodium ion per monomer.UniRule annotation
    Binds 2 magnesium ions per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei101 – 1011SubstrateUniRule annotation
    Metal bindingi200 – 2001SodiumUniRule annotation
    Binding sitei200 – 2001SubstrateUniRule annotation
    Sitei356 – 3561Transition state stabilizerUniRule annotation
    Sitei390 – 3901Transition state stabilizerUniRule annotation
    Metal bindingi415 – 4151Magnesium 1UniRule annotation
    Metal bindingi417 – 4171Magnesium 1UniRule annotation
    Active sitei460 – 4601Proton donorUniRule annotation
    Metal bindingi460 – 4601Magnesium 1UniRule annotation
    Binding sitei460 – 4601SubstrateUniRule annotation
    Active sitei536 – 5361NucleophileUniRule annotation
    Metal bindingi596 – 5961Magnesium 2UniRule annotation
    Metal bindingi600 – 6001Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi603 – 6031SodiumUniRule annotation
    Binding sitei603 – 6031SubstrateUniRule annotation
    Binding sitei998 – 9981SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotationSAAS annotation, Hydrolase

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation, SodiumUniRule annotation

    Enzyme and pathway databases

    BioCyciECLO716541:GH13-3731-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:ECL_03691Imported
    OrganismiEnterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)Imported
    Taxonomic identifieri716541 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
    ProteomesiUP000002363: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD5C6R3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni536 – 5394Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000252443.
    KOiK01190.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D5C6R3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MITDSLAVVL QRRDWENPGV TQLNRLAAHP PFASWRNSEE ARTDRPSQES     50
    RSLNGEWRFA WFPAPEAVPE SWLERDLPDA DTVIVPSNWQ MHGYDAPIYT 100
    NVTYPIAVNP PYVPTENPTG CYSLTFNVDE SWLQEGQTRI IFDGVNSAFH 150
    LWCNGRWVGY GQDSRLPSEF DLSAFLHAGE NRLAVMVLRW SDGSYLEDQD 200
    MWRMSGIFRD VSLLHKPSTQ ISDFHVATHF NDDFSRAVLE ADVQMYGELR 250
    DELRVTVSLW QGETQVASGT APFGGEIIDE RGGYADHVTL RLNVENPKLW 300
    SAEIPNLYRA VVELHTADGT LIEAEACDVG FREVRIENGL LLLNGKPLLI 350
    RGVNRHEHHP LHGQVMDEQT MVQDILLMKQ NNFNAVRCSH YPNHPLWYTL 400
    CDHYGLYVVD EANIETHGMV PMNRLTDDPR WLPAMSERVT RMVQRDRNHP 450
    SVIIWSLGNE SGHGANHDAL YRWIKSVDPS RPVQYEGGGA DTFATDIICP 500
    MYARVDEDQP FPAVPKWSIK KWLSLPGETR PLILCEYAHA MGNSLGGFAK 550
    YWQAFRQYPR LQGGFVWDWV DQSLIKYDEN GNPWSAYGGD FGDKPNDRQF 600
    CMNGLVFADR TPHPALTEAK HQQQFFQFSL SGRTIEVTSE YLFRHSDNEL 650
    LHWMVALDGK PLASGEVPLD VAPQGKQLIE LPELPQPKSA GQLWLTVHVV 700
    QPNATTWSAA GHISAWQQWR LAENLSVTLP SAPHAIPQLT TSETDFCIEL 750
    DNKRWQFNRQ SGFLSQMWIG DKKQLLTPLR DQFTRAPLDN DIGVSEATRI 800
    DPNAWVERWK AAGHYQAEAA LLQCTADTLA DAVLITTVHA WQHQGKTLFI 850
    SRKTYRIDGS GQMAITVDVE VASNTPHPAR IGLTCQLAQV AERVNWLGLG 900
    PQENYPDRLT AACFDRWDLP LSDMYTPYVF PSENGLRCGT RELNYGPHQW 950
    RGDFQFNISR YSQQQLMETS HRHLLHAEEG TWLNIDGFHM GIGGDDSWSP 1000
    SVSAEFHLSA GSYHYQLLWC QK 1022
    Length:1,022
    Mass (Da):116,183
    Last modified:June 15, 2010 - v1
    Checksum:i1B8314BA008F69EF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001918 Genomic DNA. Translation: ADF63225.1.
    RefSeqiWP_013098161.1. NC_014121.1.
    YP_003614174.1. NC_014121.1.

    Genome annotation databases

    EnsemblBacteriaiADF63225; ADF63225; ECL_03691.
    GeneIDi9126168.
    KEGGienc:ECL_03691.
    PATRICi38458276. VBIEntClo148801_3849.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001918 Genomic DNA. Translation: ADF63225.1 .
    RefSeqi WP_013098161.1. NC_014121.1.
    YP_003614174.1. NC_014121.1.

    3D structure databases

    ProteinModelPortali D5C6R3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADF63225 ; ADF63225 ; ECL_03691 .
    GeneIDi 9126168.
    KEGGi enc:ECL_03691.
    PATRICi 38458276. VBIEntClo148801_3849.

    Phylogenomic databases

    HOGENOMi HOG000252443.
    KOi K01190.

    Enzyme and pathway databases

    BioCyci ECLO716541:GH13-3731-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Enterobacter cloacae subsp. cloacae type strain ATCC 13047."
      Ren Y., Ren Y., Zhou Z., Guo X., Li Y., Feng L., Wang L.
      J. Bacteriol. 192:2463-2464(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56Imported.

    Entry informationi

    Entry nameiD5C6R3_ENTCC
    AccessioniPrimary (citable) accession number: D5C6R3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 15, 2010
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 28 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3