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D5C6R3

- D5C6R3_ENTCC

UniProt

D5C6R3 - D5C6R3_ENTCC

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Protein
Beta-galactosidase
Gene
lacZ, ECL_03691
Organism
Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotationSAAS annotations

Cofactori

Binds 1 sodium ion per monomer By similarity.UniRule annotation
Binds 2 magnesium ions per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011Substrate By similarityUniRule annotation
Metal bindingi200 – 2001Sodium By similarityUniRule annotation
Binding sitei200 – 2001Substrate By similarityUniRule annotation
Sitei356 – 3561Transition state stabilizer By similarityUniRule annotation
Sitei390 – 3901Transition state stabilizer By similarityUniRule annotation
Metal bindingi415 – 4151Magnesium 1 By similarityUniRule annotation
Metal bindingi417 – 4171Magnesium 1 By similarityUniRule annotation
Active sitei460 – 4601Proton donor By similarityUniRule annotation
Metal bindingi460 – 4601Magnesium 1 By similarityUniRule annotation
Binding sitei460 – 4601Substrate By similarityUniRule annotation
Active sitei536 – 5361Nucleophile By similarityUniRule annotation
Metal bindingi596 – 5961Magnesium 2 By similarityUniRule annotation
Metal bindingi600 – 6001Sodium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi603 – 6031Sodium By similarityUniRule annotation
Binding sitei603 – 6031Substrate By similarityUniRule annotation
Binding sitei998 – 9981Substrate By similarityUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationSAAS annotations, Hydrolase

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, SodiumUniRule annotation

Enzyme and pathway databases

BioCyciECLO716541:GH13-3731-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
Lactase
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:ECL_03691Imported
OrganismiEnterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)Imported
Taxonomic identifieri716541 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
ProteomesiUP000002363: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD5C6R3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 5394Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252443.
KOiK01190.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5C6R3-1 [UniParc]FASTAAdd to Basket

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MITDSLAVVL QRRDWENPGV TQLNRLAAHP PFASWRNSEE ARTDRPSQES     50
RSLNGEWRFA WFPAPEAVPE SWLERDLPDA DTVIVPSNWQ MHGYDAPIYT 100
NVTYPIAVNP PYVPTENPTG CYSLTFNVDE SWLQEGQTRI IFDGVNSAFH 150
LWCNGRWVGY GQDSRLPSEF DLSAFLHAGE NRLAVMVLRW SDGSYLEDQD 200
MWRMSGIFRD VSLLHKPSTQ ISDFHVATHF NDDFSRAVLE ADVQMYGELR 250
DELRVTVSLW QGETQVASGT APFGGEIIDE RGGYADHVTL RLNVENPKLW 300
SAEIPNLYRA VVELHTADGT LIEAEACDVG FREVRIENGL LLLNGKPLLI 350
RGVNRHEHHP LHGQVMDEQT MVQDILLMKQ NNFNAVRCSH YPNHPLWYTL 400
CDHYGLYVVD EANIETHGMV PMNRLTDDPR WLPAMSERVT RMVQRDRNHP 450
SVIIWSLGNE SGHGANHDAL YRWIKSVDPS RPVQYEGGGA DTFATDIICP 500
MYARVDEDQP FPAVPKWSIK KWLSLPGETR PLILCEYAHA MGNSLGGFAK 550
YWQAFRQYPR LQGGFVWDWV DQSLIKYDEN GNPWSAYGGD FGDKPNDRQF 600
CMNGLVFADR TPHPALTEAK HQQQFFQFSL SGRTIEVTSE YLFRHSDNEL 650
LHWMVALDGK PLASGEVPLD VAPQGKQLIE LPELPQPKSA GQLWLTVHVV 700
QPNATTWSAA GHISAWQQWR LAENLSVTLP SAPHAIPQLT TSETDFCIEL 750
DNKRWQFNRQ SGFLSQMWIG DKKQLLTPLR DQFTRAPLDN DIGVSEATRI 800
DPNAWVERWK AAGHYQAEAA LLQCTADTLA DAVLITTVHA WQHQGKTLFI 850
SRKTYRIDGS GQMAITVDVE VASNTPHPAR IGLTCQLAQV AERVNWLGLG 900
PQENYPDRLT AACFDRWDLP LSDMYTPYVF PSENGLRCGT RELNYGPHQW 950
RGDFQFNISR YSQQQLMETS HRHLLHAEEG TWLNIDGFHM GIGGDDSWSP 1000
SVSAEFHLSA GSYHYQLLWC QK 1022
Length:1,022
Mass (Da):116,183
Last modified:June 15, 2010 - v1
Checksum:i1B8314BA008F69EF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001918 Genomic DNA. Translation: ADF63225.1.
RefSeqiWP_013098161.1. NC_014121.1.
YP_003614174.1. NC_014121.1.

Genome annotation databases

EnsemblBacteriaiADF63225; ADF63225; ECL_03691.
GeneIDi9126168.
KEGGienc:ECL_03691.
PATRICi38458276. VBIEntClo148801_3849.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001918 Genomic DNA. Translation: ADF63225.1 .
RefSeqi WP_013098161.1. NC_014121.1.
YP_003614174.1. NC_014121.1.

3D structure databases

ProteinModelPortali D5C6R3.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADF63225 ; ADF63225 ; ECL_03691 .
GeneIDi 9126168.
KEGGi enc:ECL_03691.
PATRICi 38458276. VBIEntClo148801_3849.

Phylogenomic databases

HOGENOMi HOG000252443.
KOi K01190.

Enzyme and pathway databases

BioCyci ECLO716541:GH13-3731-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Enterobacter cloacae subsp. cloacae type strain ATCC 13047."
    Ren Y., Ren Y., Zhou Z., Guo X., Li Y., Feng L., Wang L.
    J. Bacteriol. 192:2463-2464(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56.

Entry informationi

Entry nameiD5C6R3_ENTCC
AccessioniPrimary (citable) accession number: D5C6R3
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: September 3, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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