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D5C1B7 (D5C1B7_NITHN) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338

Short name=RuBisCO large subunit HAMAP-Rule MF_01338
EC=4.1.1.39 HAMAP-Rule MF_01338
Gene names
Name:cbbL HAMAP-Rule MF_01338
Ordered Locus Names:Nhal_3442 EMBL ADE16469.1
OrganismNitrosococcus halophilus (strain Nc4) [Complete proteome] [HAMAP] EMBL ADE16469.1
Taxonomic identifier472759 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains By similarity. HAMAP-Rule MF_01338

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity. HAMAP-Rule MF_01338

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily. HAMAP-Rule MF_01338

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1831Proton acceptor By similarity HAMAP-Rule MF_01338
Active site3011Proton acceptor By similarity HAMAP-Rule MF_01338
Metal binding2091Magnesium; via carbamate group By similarity HAMAP-Rule MF_01338
Metal binding2111Magnesium By similarity HAMAP-Rule MF_01338
Metal binding2121Magnesium By similarity HAMAP-Rule MF_01338
Binding site1311Substrate; in homodimeric partner By similarity HAMAP-Rule MF_01338
Binding site1811Substrate By similarity HAMAP-Rule MF_01338
Binding site1851Substrate By similarity HAMAP-Rule MF_01338
Binding site3021Substrate By similarity HAMAP-Rule MF_01338
Binding site3341Substrate By similarity HAMAP-Rule MF_01338
Binding site3861Substrate By similarity HAMAP-Rule MF_01338
Site3411Transition state stabilizer By similarity HAMAP-Rule MF_01338

Amino acid modifications

Modified residue2091N6-carboxylysine By similarity HAMAP-Rule MF_01338

Sequences

Sequence LengthMass (Da)Tools
D5C1B7 [UniParc].

Last modified June 15, 2010. Version 1.
Checksum: 5620AFFE070F1BAB

FASTA49254,513
        10         20         30         40         50         60 
MGKSETMAEG KERYKSGVIP YKKMGYWEPD YKPKETDIIA MFRITPQAGV DPAEAAAAVA 

        70         80         90        100        110        120 
GESSTATWTV VWTDRLTACE LYRAKAYRVD PVPNTGEGTK TEAQYFAYIA YDLDLFEPGS 

       130        140        150        160        170        180 
IANLTASIIG NVFGFKAVKA LRLEDMHIPV AYLKTFQGPA TGVIVERERL DKFGRPLLGA 

       190        200        210        220        230        240 
TTKPKLGLSG RNYGRVVYEA LKGGLDFVKD DENINSQPFM HWRDRFLYCM EAVNKASAAT 

       250        260        270        280        290        300 
GEVKGHYLNV TAATMEDMYE RAEFAKSLGS VIIMIDLVVG YTAIQSMAKW ARKNDMVLHL 

       310        320        330        340        350        360 
HRAGNSTYSR QKNHGMNFRV ICKWMRMAGV DHIHAGTVVG KLEGDPLMIK GFYDTLRERH 

       370        380        390        400        410        420 
TPTSLEHGLF FDQDWASLNK VMPVASGGIH AGQMHQLLHY LGEDVVLQFG GGTIGHPQGI 

       430        440        450        460        470        480 
QAGATANRVA LEAMIMARNE GRDYMKEGPQ ILEDAAKWCS PLKAALDTWK DITFNYESTD 

       490 
TADFVPTATT SV 

« Hide

References

[1]"Complete genome sequence of Nitrosococcus halophilus Nc4, a salt-adapted, aerobic obligate ammonia-oxidizing sulfur purple bacterium."
US DOE Joint Genome Institute
Campbell M.A., Malfatti S.A., Chain P.S.G., Heidelberg J.F., Ward B.B., Klotz M.G.
Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nc4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001798 Genomic DNA. Translation: ADE16469.1.
RefSeqYP_003528856.1. NC_013960.1.

3D structure databases

ProteinModelPortalD5C1B7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE16469; ADE16469; Nhal_3442.
GeneID8918453.
KEGGnhl:Nhal_3442.
PATRIC35397002. VBINitHal115488_3612.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000230831.
KOK01601.

Enzyme and pathway databases

BioCycNHAL472759:GHH2-3489-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD5C1B7_NITHN
AccessionPrimary (citable) accession number: D5C1B7
Entry history
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: June 11, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)