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Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Nitrosococcus halophilus (strain Nc4)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei131 – 1311Substrate; in homodimeric partnerUniRule annotation
Binding sitei181 – 1811SubstrateUniRule annotation
Active sitei183 – 1831Proton acceptorUniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotation
Metal bindingi209 – 2091Magnesium; via carbamate groupUniRule annotation
Metal bindingi211 – 2111MagnesiumUniRule annotation
Metal bindingi212 – 2121MagnesiumUniRule annotation
Active sitei301 – 3011Proton acceptorUniRule annotation
Binding sitei302 – 3021SubstrateUniRule annotation
Binding sitei334 – 3341SubstrateUniRule annotation
Sitei341 – 3411Transition state stabilizerUniRule annotation
Binding sitei386 – 3861SubstrateUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. monooxygenase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. reductive pentose-phosphate cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, MonooxygenaseUniRule annotation, Oxidoreductase

Keywords - Biological processi

Calvin cycleUniRule annotation, Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciNHAL472759:GHH2-3489-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:cbbLUniRule annotation
Ordered Locus Names:Nhal_3442Imported
OrganismiNitrosococcus halophilus (strain Nc4)Imported
Taxonomic identifieri472759 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
ProteomesiUP000001844 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei209 – 2091N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD5C1B7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230831.
KOiK01601.
OMAiITAGWMA.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5C1B7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSETMAEG KERYKSGVIP YKKMGYWEPD YKPKETDIIA MFRITPQAGV
60 70 80 90 100
DPAEAAAAVA GESSTATWTV VWTDRLTACE LYRAKAYRVD PVPNTGEGTK
110 120 130 140 150
TEAQYFAYIA YDLDLFEPGS IANLTASIIG NVFGFKAVKA LRLEDMHIPV
160 170 180 190 200
AYLKTFQGPA TGVIVERERL DKFGRPLLGA TTKPKLGLSG RNYGRVVYEA
210 220 230 240 250
LKGGLDFVKD DENINSQPFM HWRDRFLYCM EAVNKASAAT GEVKGHYLNV
260 270 280 290 300
TAATMEDMYE RAEFAKSLGS VIIMIDLVVG YTAIQSMAKW ARKNDMVLHL
310 320 330 340 350
HRAGNSTYSR QKNHGMNFRV ICKWMRMAGV DHIHAGTVVG KLEGDPLMIK
360 370 380 390 400
GFYDTLRERH TPTSLEHGLF FDQDWASLNK VMPVASGGIH AGQMHQLLHY
410 420 430 440 450
LGEDVVLQFG GGTIGHPQGI QAGATANRVA LEAMIMARNE GRDYMKEGPQ
460 470 480 490
ILEDAAKWCS PLKAALDTWK DITFNYESTD TADFVPTATT SV
Length:492
Mass (Da):54,513
Last modified:June 14, 2010 - v1
Checksum:i5620AFFE070F1BAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001798 Genomic DNA. Translation: ADE16469.1.
RefSeqiYP_003528856.1. NC_013960.1.

Genome annotation databases

EnsemblBacteriaiADE16469; ADE16469; Nhal_3442.
KEGGinhl:Nhal_3442.
PATRICi35397002. VBINitHal115488_3612.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001798 Genomic DNA. Translation: ADE16469.1.
RefSeqiYP_003528856.1. NC_013960.1.

3D structure databases

ProteinModelPortaliD5C1B7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE16469; ADE16469; Nhal_3442.
KEGGinhl:Nhal_3442.
PATRICi35397002. VBINitHal115488_3612.

Phylogenomic databases

HOGENOMiHOG000230831.
KOiK01601.
OMAiITAGWMA.

Enzyme and pathway databases

BioCyciNHAL472759:GHH2-3489-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Nitrosococcus halophilus Nc4, a salt-adapted, aerobic obligate ammonia-oxidizing sulfur purple bacterium."
    US DOE Joint Genome Institute
    Campbell M.A., Malfatti S.A., Chain P.S.G., Heidelberg J.F., Ward B.B., Klotz M.G.
    Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nc4Imported.

Entry informationi

Entry nameiD5C1B7_NITHN
AccessioniPrimary (citable) accession number: D5C1B7
Entry historyi
Integrated into UniProtKB/TrEMBL: June 14, 2010
Last sequence update: June 14, 2010
Last modified: March 31, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.