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D5BUE2 (D5BUE2_PUNMI) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129
Alternative name(s):
Glucose-inhibited division protein A HAMAP-Rule MF_00129
Gene names
Name:gidA HAMAP-Rule MF_00129
Synonyms:mnmG HAMAP-Rule MF_00129
Ordered Locus Names:SAR116_1646 EMBL ADE39889.1
OrganismPuniceispirillum marinum (strain IMCC1322) [Complete proteome] [HAMAP] EMBL ADE39889.1
Taxonomic identifier488538 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › SAR116 cluster › Candidatus Puniceispirillum ›

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129

Cofactor

FAD By similarity. HAMAP-Rule MF_00129 SAAS SAAS026904

Subunit structure

Homodimer. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP-Rule MF_00129

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00129.

Sequence similarities

Belongs to the MnmG family. HAMAP-Rule MF_00129

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding11 – 166FAD By similarity HAMAP-Rule MF_00129
Nucleotide binding279 – 29315NAD By similarity HAMAP-Rule MF_00129

Sites

Binding site1231FAD; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_00129
Binding site1871FAD By similarity HAMAP-Rule MF_00129
Binding site3761FAD By similarity HAMAP-Rule MF_00129

Sequences

Sequence LengthMass (Da)Tools
D5BUE2 [UniParc].

Last modified June 15, 2010. Version 1.
Checksum: D71B4FA8A3E301F5

FASTA64869,860
        10         20         30         40         50         60 
MKSAFDVIVV GGGHAGCEAA AAASRMGAAV ALVTMRADRI GEMSCNPAIG GLGKGHLVRE 

        70         80         90        100        110        120 
IDALDGLMGR AIDRAGIQFR MLNKSRGPAV HGPRAQADRA LYRAAIQEFL NEDDHITIIE 

       130        140        150        160        170        180 
AAVGDLLVDD MAAGLTGAPR QVTGIICEDG RELKAASVVL TTGTFLGGLI HLGNERTEAG 

       190        200        210        220        230        240 
RIGEAPSNML ATRLADLALP LGRLKTGTPP RLDGRTIDWS VLEMQPADNP PIPFSTMTDK 

       250        260        270        280        290        300 
ITVPQIECGI TRTTAKTHRI IAESIHLSAV FNGNISGQGP RYCPSIEDKV NRFADKDSHQ 

       310        320        330        340        350        360 
LFLEPEGLDD HTIYPNGIST SLPRDVQEAL VKTIPGLEQT VILQHGYAIE YNFVDPRALR 

       370        380        390        400        410        420 
QTLELRVLPG LYLAGQINGT TGYEEAAAQG LMAGVNAALA AASGDNRQKT SFTLDRADAY 

       430        440        450        460        470        480 
IGVMIDDLIS KGAPEPYRMF TSRAEYRLLL RADNADQRLT EKGMAIGCVG SARQNVWHRK 

       490        500        510        520        530        540 
HKQLAKAKAT LLSLTASGKD LEIAGLPKPR DGGKRTAADM LSLEGITIDR LTPLWPELAN 

       550        560        570        580        590        600 
IASLYHAQLE ADYRYAGYVA RQKADIDALR RDEATAIPDD LDFSVIGGLS AEAQDVLKRR 

       610        620        630        640 
QPETIGQANR LAGLTPASVV AVLRHLRRRE DAAYNTKPDV SVMRRETI

« Hide

References

[1]"Complete genome sequence of "Candidatus Puniceispirillum marinum" IMCC1322, a representative of the SAR116 clade in the Alphaproteobacteria."
Oh H.M., Kwon K.K., Kang I., Kang S.G., Lee J.H., Kim S.J., Cho J.C.
J. Bacteriol. 192:3240-3241(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IMCC1322 EMBL ADE39889.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001751 Genomic DNA. Translation: ADE39889.1.
RefSeqYP_003551973.1. NC_014010.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE39889; ADE39889; SAR116_1646.
GeneID8961983.
KEGGapb:SAR116_1646.
PATRIC35450263. VBIAlpPro78664_1666.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000201059.
KOK03495.
OMAAQMSCNP.

Enzyme and pathway databases

BioCycPMAR488538:GI1I-1644-MONOMER.

Family and domain databases

HAMAPMF_00129. MnmG_GidA.
InterProIPR004416. GidA.
IPR026904. GidA-assoc_3.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc_3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00136. gidA. 1 hit.
PROSITEPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD5BUE2_PUNMI
AccessionPrimary (citable) accession number: D5BUE2
Entry history
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info