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Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Zunongwangia profunda (strain DSM 18752 / CCTCC AB 206139 / SM-A87)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561NucleophileUniRule annotation

GO - Molecular functioni

  1. pseudouridine synthase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: InterPro

GO - Biological processi

  1. RNA processing Source: InterPro
  2. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciZPRO655815:GI6J-4437-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:ZPR_4442Imported
OrganismiZunongwangia profunda (strain DSM 18752 / CCTCC AB 206139 / SM-A87)Imported
Taxonomic identifieri655815 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeZunongwangia
ProteomesiUP000001654 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliD5BCB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000231223.
KOiK03177.
OMAiHATKISG.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

D5BCB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHPIITAEE FKTGQILLFD KPLNWTSFQL VNKVRWMIRK SCDIKKIKVG
60 70 80 90 100
HAGTLDPLAT GLLIICTGKF TKRIPELQGQ IKEYTGTFCL GSTTASYDLE
110 120 130 140 150
TEIDETFPID HITEENIAET TKSFIGDIEQ LPPVFSALKK DGKRLYEYAR
160 170 180 190 200
KGEEVEIKPR TISIEAFETD ASDFPDINFR VVCSKGTYIR SLAHDFGKKL
210 220 230
NSGAHLSSLR RTKIGDFSIE NSLGLDDFEK QLPRPVSGA
Length:239
Mass (Da):26,864
Last modified:June 15, 2010 - v1
Checksum:iFEE066F92F81E6E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001650 Genomic DNA. Translation: ADF54743.1.
RefSeqiYP_003586939.1. NC_014041.1.

Genome annotation databases

EnsemblBacteriaiADF54743; ADF54743; ZPR_4442.
KEGGizpr:ZPR_4442.
PATRICi37238188. VBIZunPro130839_4288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001650 Genomic DNA. Translation: ADF54743.1.
RefSeqiYP_003586939.1. NC_014041.1.

3D structure databases

ProteinModelPortaliD5BCB7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF54743; ADF54743; ZPR_4442.
KEGGizpr:ZPR_4442.
PATRICi37238188. VBIZunPro130839_4288.

Phylogenomic databases

HOGENOMiHOG000231223.
KOiK03177.
OMAiHATKISG.

Enzyme and pathway databases

BioCyciZPRO655815:GI6J-4437-MONOMER.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome of Zunongwangia profunda SM-A87 reveals its adaptation to the deep-sea environment and ecological role in sedimentary organic nitrogen degradation."
    Qin Q.L., Zhang X.Y., Wang X.M., Liu G.M., Chen X.L., Xie B.B., Dang H.Y., Zhou B.C., Yu J., Zhang Y.Z.
    BMC Genomics 11:247-247(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 18752 / CCTCC AB 206139 / SM-A87Imported.

Entry informationi

Entry nameiD5BCB7_ZUNPS
AccessioniPrimary (citable) accession number: D5BCB7
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: April 29, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.