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Protein

Ribulose bisphosphate carboxylase

Gene

cbbM

Organism
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site (By similarity).By similarity

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111Substrate; in homodimeric partnerBy similarity
Active sitei166 – 1661Proton acceptorBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Metal bindingi191 – 1911Magnesium; via carbamate groupBy similarity
Metal bindingi193 – 1931MagnesiumBy similarity
Metal bindingi194 – 1941MagnesiumBy similarity
Active sitei287 – 2871Proton acceptorBy similarity
Binding sitei288 – 2881SubstrateBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Sitei329 – 3291Transition state stabilizerBy similarity
Binding sitei368 – 3681SubstrateBy similarity

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. monooxygenase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. reductive pentose-phosphate cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Calvin cycle, Carbon dioxide fixation, Photosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRCAP272942:GJIY-1854-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase (EC:4.1.1.39)
Short name:
RuBisCO
Gene namesi
Name:cbbM
Ordered Locus Names:RCAP_rcc01829
OrganismiRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Taxonomic identifieri272942 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002361: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458Ribulose bisphosphate carboxylasePRO_0000410720Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911N6-carboxylysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliD5AUD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000230831.
KOiK01601.
OMAiNQYLHYH.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01339. RuBisCO_L_type2.
InterProiIPR020871. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5AUD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQSNRYARL DLKEADLIAG GRHVLCAYVM KPKAGYGYLE TAAHFAAESS
60 70 80 90 100
TGTNVEVSTT DDFTRGVDAL VYEIDPEKEI MKIAYPVELF DRNIIDGRAM
110 120 130 140 150
LCSFLTLTIG NNQGMGDVEY AKMHEFYVPP CYLRLFDGPS MNIADMWRVL
160 170 180 190 200
GRPVVDGGMV VGTIIKPKLG LRPKPFADAC YEFWLGGDFI KNDEPQGNQT
210 220 230 240 250
FAPLKETIRL VADAMKRAQD ETGEAKLFSA NITADDHYEM VARGEYILET
260 270 280 290 300
FGENADHVAF LVDGYVTGPA AITTARRQFP RQFLHYHRAG HGAVTSPQSM
310 320 330 340 350
RGYTAFVLSK MSRLQGASGI HTGTMGYGKM EGDASDKIMA YMLTDEAAQG
360 370 380 390 400
PFYHQDWLGM KATTPIISGG MNALRLPGFF DNLGHSNVIQ TSGGGAFGHL
410 420 430 440 450
DGGTAGAKSL RQSCDAWKAG VDLVTYAKSH RELARAFESF PNDADKLYPG

WRVALGVN
Length:458
Mass (Da):50,205
Last modified:June 15, 2010 - v1
Checksum:iA03E0FF321EF3B6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001312 Genomic DNA. Translation: ADE85574.1.
RefSeqiYP_003577981.1. NC_014034.1.

Genome annotation databases

EnsemblBacteriaiADE85574; ADE85574; RCAP_rcc01829.
GeneIDi9004652.
KEGGircp:RCAP_rcc01829.
PATRICi35503806. VBIRhoCap134200_1861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001312 Genomic DNA. Translation: ADE85574.1.
RefSeqiYP_003577981.1. NC_014034.1.

3D structure databases

ProteinModelPortaliD5AUD5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE85574; ADE85574; RCAP_rcc01829.
GeneIDi9004652.
KEGGircp:RCAP_rcc01829.
PATRICi35503806. VBIRhoCap134200_1861.

Phylogenomic databases

HOGENOMiHOG000230831.
KOiK01601.
OMAiNQYLHYH.

Enzyme and pathway databases

BioCyciRCAP272942:GJIY-1854-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01339. RuBisCO_L_type2.
InterProiIPR020871. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003."
    Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R.
    J. Bacteriol. 192:3545-3546(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
  2. "Expression of the cbbLcbbS and cbbM genes and distinct organization of the cbb Calvin cycle structural genes of Rhodobacter capsulatus."
    Paoli G.C., Morgan N.S., Tabita F.R., Shively J.M.
    Arch. Microbiol. 164:396-405(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: OPERON ORGANIZATION, EXPRESSION IN R.SPHAEROIDES.
    Strain: ATCC BAA-309 / NBRC 16581 / SB1003.

Entry informationi

Entry nameiRBL2_RHOCB
AccessioniPrimary (citable) accession number: D5AUD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 15, 2010
Last modified: March 4, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In contrast to form I RuBisCO, the form II RuBisCO are composed solely of large subunits (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.