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D4P095

- CPDA_PSEAI

UniProt

D4P095 - CPDA_PSEAI

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Protein

3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA

Gene
cpdA
Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes. Specifically required for regulation of virulence factors. Can also hydrolyze cGMP.1 Publication

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.1 Publication

Cofactori

Binds 2 metal cations per subunit. Site 1 may preferentially bind Fe3+ ions, while site 2 may have a preference for Fe2+ ions.1 Publication

Enzyme regulationi

Activated by iron. Other divalent metal ions have no effect.1 Publication

Kineticsi

  1. KM=7.2 µM for cAMP1 Publication

Vmax=3.4 nmol/min/ng enzyme

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi21 – 211Iron 1 By similarity
Metal bindingi23 – 231Iron 1 Inferred
Binding sitei23 – 231cAMP By similarity
Metal bindingi63 – 631Iron 1 Inferred
Metal bindingi63 – 631Iron 2 By similarity
Binding sitei63 – 631cAMP By similarity
Metal bindingi93 – 931Iron 2 Inferred
Metal bindingi161 – 1611Iron 2 By similarity
Metal bindingi200 – 2001Iron 2 By similarity
Metal bindingi202 – 2021Iron 1 By similarity
Binding sitei202 – 2021cAMP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 942cAMP By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-HAMAP
  3. nucleotide binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Iron, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA (EC:3.1.4.17)
Short name:
3',5'-cyclic AMP phosphodiesterase
Short name:
cAMP phosphodiesterase
Gene namesi
Name:cpdA
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

Disruption phenotypei

Mutants show increased levels of cellular cAMP. In rich medium, mutants exhibit a significantly reduced growth rate compared to wild-type strain.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231H → A: Loss of activity. 1 Publication
Mutagenesisi63 – 631D → A: Loss of activity. 1 Publication
Mutagenesisi93 – 931N → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2722723',5'-cyclic adenosine monophosphate phosphodiesterase CpdAUniRule annotationPRO_0000413373Add
BLAST

Expressioni

Inductioni

Positively regulated by Vfr in response to elevated intracellular cAMP.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliD4P095.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
HAMAPiMF_00905. cAMP_phophodiest_CpdA.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR026575. cAMP_Pdiest_CpdA.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

D4P095-1 [UniParc]FASTAAdd to Basket

« Hide

MSRHSNTPAT DASVLLVQLS DSHLFAEDGA RLLGMDTAHS LEKVVERVAR    50
EQPRIDLILA TGDVSQDGSL DSYTRFRRLS APLAAPLRWF AGNHDEREPM 100
QRATEGSDLL EQIVDVGNWR VVLLDSSIPG AVPGYLEDDQ LDLLRRAIDS 150
AGERFLLVSF HHHPVPIGSD WMDPIGLRNP QALFDLLAPY PQLRCLLWGH 200
IHQEFDRQRG PLRLLASPST CVQFAPGSSD FTLDRLAPGY RWLRLHDDGR 250
LETGISRVDD VVFEVDYDTA GY 272
Length:272
Mass (Da):30,472
Last modified:May 18, 2010 - v1
Checksum:i1C22AEBA58FD867F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GU551724 Genomic DNA. Translation: ADD69827.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GU551724 Genomic DNA. Translation: ADD69827.1 .

3D structure databases

ProteinModelPortali D4P095.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
HAMAPi MF_00905. cAMP_phophodiest_CpdA.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR026575. cAMP_Pdiest_CpdA.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "In vitro and in vivo characterization of the Pseudomonas aeruginosa cyclic AMP (cAMP) phosphodiesterase CpdA, required for cAMP homeostasis and virulence factor regulation."
    Fuchs E.L., Brutinel E.D., Klem E.R., Fehr A.R., Yahr T.L., Wolfgang M.C.
    J. Bacteriol. 192:2779-2790(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INDUCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF HIS-23; ASP-63 AND ASN-93.
    Strain: PAK.

Entry informationi

Entry nameiCPDA_PSEAI
AccessioniPrimary (citable) accession number: D4P095
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: May 18, 2010
Last modified: June 11, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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