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D4IFZ5 (D4IFZ5_ERWAE) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlB EMBL CBJ45375.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:EAM_0700 EMBL CBJ45375.1
OrganismErwinia amylovora (strain ATCC 49946 / CCPPB 0273 / Ea273 / 27-3) [Complete proteome] [HAMAP] EMBL CBJ45375.1
Taxonomic identifier716540 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain101 – 303203ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding134 – 18956ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2571Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2721Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D4IFZ5 [UniParc].

Last modified May 18, 2010. Version 1.
Checksum: 18AB0D5C1256B093

FASTA30632,871
        10         20         30         40         50         60 
MAEKVAVLLG GTSAERDVSL LSGTAVLKGL QEAGIDAYPV DIRDFPVTRL KEEGFEKAFI 

        70         80         90        100        110        120 
ALHGRGGEDG TLQGVLEFLN IPYTGSGVMA SAITMDKLRS KYLWQGCGLP VSPFVALTRA 

       130        140        150        160        170        180 
QMDAGLDAQV MASIDALGLP LFVKPSREGS SVGISRVNQA SELQAALEEA FRFDHEVLVE 

       190        200        210        220        230        240 
AFLSGPEYTV GVLGTDILPS IRIQTASEFY DYDAKYFSDE TQYFCPSGLS ADQEAELREL 

       250        260        270        280        290        300 
TISAWNALGC SGWGRVDVMM GGDGRFYLLE VNTSPGMTSH SLVPMAAKQA GMSFSQLVAR 


ILELAD 

« Hide

References

[1]"Complete genome sequence of the plant pathogen Erwinia amylovora strain ATCC 49946."
Sebaihia M., Bocsanczy A.M., Biehl B.S., Quail M.A., Perna N.T., Glasner J.D., DeClerck G.A., Cartinhour S., Schneider D.J., Bentley S.D., Parkhill J., Beer S.V.
J. Bacteriol. 192:2020-2021(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49946 / CCPPB 0273 / Ea273 / 27-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FN666575 Genomic DNA. Translation: CBJ45375.1.
RefSeqYP_003537790.1. NC_013971.1.

3D structure databases

ProteinModelPortalD4IFZ5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBJ45375; CBJ45375; EAM_0700.
GeneID8951516.
KEGGeay:EAM_0700.
PATRIC35412817. VBIErwAmy154257_0677.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.

Enzyme and pathway databases

BioCycEAMY716540:GJAV-737-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD4IFZ5_ERWAE
AccessionPrimary (citable) accession number: D4IFZ5
Entry history
Integrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: February 19, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)