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D4I9D5 (D4I9D5_ERWAE) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Signal recognition particle protein HAMAP-Rule MF_00306
Alternative name(s):
Fifty-four homolog HAMAP-Rule MF_00306
Gene names
Name:ffh HAMAP-Rule MF_00306 EMBL CBJ47304.1
Ordered Locus Names:EAM_2630 EMBL CBJ47304.1
OrganismErwinia amylovora (strain ATCC 49946 / CCPPB 0273 / Ea273 / 27-3) [Complete proteome] [HAMAP] EMBL CBJ47304.1
Taxonomic identifier716540 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia ›

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components By similarity. HAMAP-Rule MF_00306

Subunit structure

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule By similarity. HAMAP-Rule MF_00306

Subcellular location

Cytoplasm. Note: The SRP-RNC complex is targeted to the cytoplasmic membrane By similarity. HAMAP-Rule MF_00306

Domain

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC By similarity. HAMAP-Rule MF_00306

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily. HAMAP-Rule MF_00306

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding107 – 1148GTP By similarity HAMAP-Rule MF_00306
Nucleotide binding190 – 1945GTP By similarity HAMAP-Rule MF_00306
Nucleotide binding248 – 2514GTP By similarity HAMAP-Rule MF_00306

Sequences

Sequence LengthMass (Da)Tools
D4I9D5 [UniParc].

Last modified May 18, 2010. Version 1.
Checksum: 76555AFCD20AF6C7

FASTA45349,863
        10         20         30         40         50         60 
MFDNLTDRLS QTLRNISGRG RLTEDNIKDT LREVRMALLE ADVALPVVRD FIGRVKERAV 

        70         80         90        100        110        120 
GQEVNKSLTP GQEFVKIVKN ELVAAMGEEN NTLNLAAQPP AVILMAGLQG AGKTTSVGKL 

       130        140        150        160        170        180 
GKFLREKHKK KVLVVSADVY RPAAIKQLET LAEQVGVDFC PSDLTQQPVD IVKNALQQAK 

       190        200        210        220        230        240 
LKFYDVLLVD TAGRLHVDEA MMDEIKQVHA AINPVETLFV VDAMTGQDAA NTAKAFNEAL 

       250        260        270        280        290        300 
PLTGVVLTKV DGDARGGAAL SIRHITGKPI KFMGVGEKTE ALEPFYPDRI ASRILGMGDM 

       310        320        330        340        350        360 
LSLIEDIESK VDRAQAEKLA SKLKKGDGFD LNDFLDQLKQ MRNMGGMASL MGKLPGMGQL 

       370        380        390        400        410        420 
PDNVKSQMDD KVLVRMEAMI NSMTRKERQK PEIIKGSRKR RIAAGSGMQV QDVNRLLKQF 

       430        440        450 
DDMQRMMKKM KKGGMAKMMR GMKGMMPPGF PGR

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References

[1]"Complete genome sequence of the plant pathogen Erwinia amylovora strain ATCC 49946."
Sebaihia M., Bocsanczy A.M., Biehl B.S., Quail M.A., Perna N.T., Glasner J.D., Declerck G.A., Cartinhour S., Schneider D.J., Bentley S.D., Parkhill J., Beer S.V.
J. Bacteriol. 192:2020-2021(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49946 / CCPPB 0273 / Ea273 / 27-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FN666575 Genomic DNA. Translation: CBJ47304.1.
RefSeqYP_003539697.1. NC_013971.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBJ47304; CBJ47304; EAM_2630.
GeneID8951957.
KEGGeay:EAM_2630.
PATRIC35416862. VBIErwAmy154257_2653.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000036164.
KOK03106.

Enzyme and pathway databases

BioCycEAMY716540:GJAV-2712-MONOMER.

Family and domain databases

Gene3D1.10.260.30. 1 hit.
HAMAPMF_00306. SRP54.
InterProIPR003593. AAA+_ATPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMSSF47446. Signal_recog_particle_SRP54_M. 1 hit.
SSF47364. SRP54. 1 hit.
TIGRFAMsTIGR00959. ffh. 1 hit.
PROSITEPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD4I9D5_ERWAE
AccessionPrimary (citable) accession number: D4I9D5
Entry history
Integrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: May 1, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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