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D4I7I2 (D4I7I2_ERWAE) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Erythronate-4-phosphate dehydrogenase HAMAP-Rule MF_01825
EC=1.1.1.290 HAMAP-Rule MF_01825
Gene names
Name:pdxB HAMAP-Rule MF_01825 EMBL CBJ47002.1
Ordered Locus Names:EAM_2328 EMBL CBJ47002.1
OrganismErwinia amylovora (strain ATCC 49946 / CCPPB 0273 / Ea273 / 27-3) [Complete proteome] [HAMAP] EMBL CBJ47002.1
Taxonomic identifier716540 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia ›

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate By similarity. HAMAP-Rule MF_01825 SAAS SAAS006139

Catalytic activity

4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH. HAMAP-Rule MF_01825 SAAS SAAS006139

Pathway

Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5. HAMAP-Rule MF_01825 SAAS SAAS006139

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01825 SAAS SAAS006139

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01825 SAAS SAAS006139.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily. HAMAP-Rule MF_01825

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2081 By similarity HAMAP-Rule MF_01825
Active site2371 By similarity HAMAP-Rule MF_01825
Active site2541Proton donor By similarity HAMAP-Rule MF_01825
Binding site451Substrate By similarity HAMAP-Rule MF_01825
Binding site661Substrate By similarity HAMAP-Rule MF_01825
Binding site1461NAD By similarity HAMAP-Rule MF_01825
Binding site1751NAD; via carbonyl oxygen By similarity HAMAP-Rule MF_01825
Binding site2321NAD By similarity HAMAP-Rule MF_01825
Binding site2571NAD; via amide nitrogen By similarity HAMAP-Rule MF_01825
Binding site2581Substrate By similarity HAMAP-Rule MF_01825

Sequences

Sequence LengthMass (Da)Tools
D4I7I2 [UniParc].

Last modified May 18, 2010. Version 1.
Checksum: 8A64571C0BB44EE9

FASTA37740,979
        10         20         30         40         50         60 
MKILVDENMP YARELFSRTG DVVAVAGRPI PQAQLEDADA LMVRSVTQVD AGLLSGKPVK 

        70         80         90        100        110        120 
FVGTATAGTD HIDEAFLQRQ GIAFSSAPGC NAIAVVEYVF SALLLLAERD GFQLTDRTVG 

       130        140        150        160        170        180 
IVGVGNVGGR LQARLAALGI RTLLCDPPRA DRGDEGDFLS LSQLVAQADV LTFHTPLFKD 

       190        200        210        220        230        240 
GPYQTRHMAD EALLCSLKPG SILINACRGA VVDNAALLKV LEQRDDLSVV LDVWEAEPDL 

       250        260        270        280        290        300 
SLPLLAKVDI ATAHIAGYTL EGKARGTTQV FEAWTQFIGQ PQQVALDTLL PAPEFGCITL 

       310        320        330        340        350        360 
SGELCQATLK RLVHLVYDVR RDDAPLRRGA AITGEFDRLR KNYHQRREWS SLRVECDNAA 

       370 
TATLLNRLGF NADHPRR

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References

[1]"Complete genome sequence of the plant pathogen Erwinia amylovora strain ATCC 49946."
Sebaihia M., Bocsanczy A.M., Biehl B.S., Quail M.A., Perna N.T., Glasner J.D., Declerck G.A., Cartinhour S., Schneider D.J., Bentley S.D., Parkhill J., Beer S.V.
J. Bacteriol. 192:2020-2021(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49946 / CCPPB 0273 / Ea273 / 27-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FN666575 Genomic DNA. Translation: CBJ47002.1.
RefSeqYP_003539399.1. NC_013971.1.

3D structure databases

ProteinModelPortalD4I7I2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBJ47002; CBJ47002; EAM_2328.
GeneID8952284.
KEGGeay:EAM_2328.
PATRIC35416180. VBIErwAmy154257_2324.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000234432.
KOK03473.

Enzyme and pathway databases

BioCycEAMY716540:GJAV-2398-MONOMER.
UniPathwayUPA00244; UER00310.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
HAMAPMF_01825. PdxB.
InterProIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR020921. Erythronate-4-P_DHase.
IPR024531. Erythronate-4-P_DHase_dimer.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR10996:SF4. PTHR10996:SF4. 1 hit.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
PF11890. DUF3410. 1 hit.
[Graphical view]
PROSITEPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD4I7I2_ERWAE
AccessionPrimary (citable) accession number: D4I7I2
Entry history
Integrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: May 1, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info