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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Erwinia amylovora (strain CFBP1430)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotationSAAS annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. dCTP deaminase (dcd)
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateUniRule annotation1
Binding sitei98Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
Biological processNucleotide metabolismUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotationSAAS annotation (EC:3.6.1.23UniRule annotationSAAS annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotationImported
Ordered Locus Names:EAMY_0074Imported
OrganismiErwinia amylovora (strain CFBP1430)Imported
Taxonomic identifieri665029 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeErwinia
Proteomesi
  • UP000001841 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi665029.EAMY_0074

Structurei

3D structure databases

ProteinModelPortaliD4HU93
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 150dUTPaseInterPro annotationAdd BLAST133

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 73Substrate bindingUniRule annotation3
Regioni88 – 90Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4108Z1K Bacteria
COG0756 LUCA
HOGENOMiHOG000028968
KOiK01520
OMAiGVILINH

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
HAMAPiMF_00116 dUTPase_bact, 1 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

D4HU93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKKIDVKVL DARVGTDFPL PTYATSGSAG LDLRACLDEA TVLPPGATTL
60 70 80 90 100
IPTGLAIHIA DPQLAAVILP RSGLGHKHGV VLGNLVGLID SDYQGQLMVS
110 120 130 140 150
VWNRGQDSFT IEPGERIAQM IFVPVVQAEF NLVNDFDASA RGEGGFGHSG

RQ
Length:152
Mass (Da):16,079
Last modified:May 18, 2010 - v1
Checksum:iCBC558BEBD73112D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN434113 Genomic DNA Translation: CBA19016.1
RefSeqiWP_004154794.1, NC_013961.1

Genome annotation databases

EnsemblBacteriaiCBA19016; CBA19016; EAMY_0074
GeneIDi8914142
KEGGieam:EAMY_0074
PATRICifig|665029.3.peg.100

Similar proteinsi

Entry informationi

Entry nameiD4HU93_ERWAC
AccessioniPrimary (citable) accession number: D4HU93
Entry historyiIntegrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: February 28, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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