ID   D4HRL2_LIMQU            Unreviewed;       457 AA.
AC   D4HRL2;
DT   18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT   18-MAY-2010, sequence version 1.
DT   24-JAN-2024, entry version 31.
DE   RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE            EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN   Name=GH9A {ECO:0000313|EMBL:ADB85440.1};
OS   Limnoria quadripunctata (Gribble).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Peracarida; Isopoda; Limnoriidae; Limnoria.
OX   NCBI_TaxID=161573 {ECO:0000313|EMBL:ADB85440.1};
RN   [1] {ECO:0000313|EMBL:ADB85440.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=20212162; DOI=10.1073/pnas.0914228107;
RA   King A.J., Cragg S.M., Li Y., Dymond J., Guille M.J., Bowles D.J.,
RA   Bruce N.C., Graham I.A., McQueen-Mason S.J.;
RT   "Molecular insight into lignocellulose digestion by a marine isopod in the
RT   absence of gut microbes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:5345-5350(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00000966,
CC         ECO:0000256|RuleBase:RU361166};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10060,
CC       ECO:0000256|RuleBase:RU361166}.
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DR   EMBL; FJ940759; ADB85440.1; -; mRNA.
DR   AlphaFoldDB; D4HRL2; -.
DR   SMR; D4HRL2; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW   ECO:0000256|PROSITE-ProRule:PRU10060};
KW   Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW   ECO:0000256|RuleBase:RU361166};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW   ProRule:PRU10060};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU10060};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW   ECO:0000256|PROSITE-ProRule:PRU10060};
KW   Signal {ECO:0000256|RuleBase:RU361166}.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000256|RuleBase:RU361166"
FT   CHAIN           18..457
FT                   /note="Endoglucanase"
FT                   /evidence="ECO:0000256|RuleBase:RU361166"
FT                   /id="PRO_5005126482"
FT   DOMAIN          33..450
FT                   /note="Glycoside hydrolase family 9"
FT                   /evidence="ECO:0000259|Pfam:PF00759"
FT   ACT_SITE        428
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        437
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ   SEQUENCE   457 AA;  48838 MW;  F12D1B18C1CB4555 CRC64;
     MKAALGILLL VLGTAYGQGN GGCSGTGMSP YDYSQALCMS PLFYEAQRSG KLPADQRVTW
     RADSGLQDGA DVGLDLTGGY YDAGDYVKFN FPMAASVTAL AFGLHAFPSG MSTAGQTNYV
     KAAVKWGSDY FLKCVQGPTT LWGQVGDPDV DHAYWGRPED MTETRVSYKI DASAPGTDLA
     AETAAALAGA AVVHSGSYAS ECVSAAEQMY TFADEHRGKY SNSIDKAANF YSSYSFHDEL
     PWAALWLYLA TGENSYLSDA EGYIQEFGLL DADVLNVDWD DKTAGVYALM VEIDGGSQYT
     SALQGYVDNL LSYQHTPGGM VYISDWGSLR EAVNAAFIAA RAASLGIKAS SYNNWAQSQA
     DYALGSIGHS YVCGFGNNPP THPHHGSSSC PVAPQPCGWN EYNSADPNPQ TLFGALVGGP
     DAQDNYVDDR DDYIHNEVAC DYNALFTGLL AAMVELH
//