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Protein

Alpha-conotoxin EIIA

Gene
N/A
Organism
Conus ermineus (Atlantic fish-hunting cone)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-conotoxins bind to the nicotinic acetylcholine receptors (nAChR) and inhibit them. This peptide potently blocks muscular nicotinic acetylcholine receptor (CHRNA1-CHRNB1-CHRNG-CHRND), and has no effect on neuronal receptors. It is able to totally displace [125I]-Bgtx from the Torpedo receptor with a complete inhibition in the high micromolar range. It produces a biphasic inhibition curve which fits nicely with a two-site model (Ki of 0.46 and 105 nM).1 Publication

Miscellaneous

Has no effect on chimeric alpha-7 (CHRNA7), alpha-3-beta-2 (CHRNA3-CHRNB2) and alpha-4-beta-2 (CHRNA4-CHRNB2).1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin EIIA
OrganismiConus ermineus (Atlantic fish-hunting cone)
Taxonomic identifieri55423 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000042288017 – 40Add BLAST24
PeptideiPRO_000042288143 – 58Alpha-conotoxin EIIAAdd BLAST16

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43Pyrrolidone carboxylic acid1 Publication1
Modified residuei45Hydroxyproline1 Publication1
Disulfide bondi47 ↔ 53By similarity
Disulfide bondi48 ↔ 58By similarity
Modified residuei58Cysteine amide1 Publication1

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond, Hydroxylation, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Expressed by the venom duct.1 Publication

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha4/4 pattern.

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR009958. Conotoxin_a-typ.
PfamiView protein in Pfam
PF07365. Toxin_8. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D4HRK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIVFLLVVL ATTVGSFTLD RVLEGRNAAA IDNALDQRDP KRQTPGCCWN
60
PACVKNRCGR R
Length:61
Mass (Da):6,779
Last modified:June 26, 2013 - v2
Checksum:i39D49248B0825395
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti29A → K. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ937346 Genomic DNA. Translation: ACZ37197.1.
FJ937347 Genomic DNA. Translation: ACZ37198.1.

Similar proteinsi

Entry informationi

Entry nameiCA2A_CONER
AccessioniPrimary (citable) accession number: D4HRK4
Secondary accession number(s): D4HRK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: June 26, 2013
Last modified: November 22, 2017
This is version 19 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families