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D4GZE7 (SAMP2_HALVD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Small archaeal modifier protein 2

Short name=SAMP 2
Alternative name(s):
Ubiquitin-like small archaeal modifier protein 2
Gene names
Ordered Locus Names:HVO_0202
OrganismHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Reference proteome] [HAMAP]
Taxonomic identifier309800 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax

Protein attributes

Sequence length66 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein modifier that is covalently attached to lysine residues of substrate proteins. The tagging system is termed SAMPylation. It is not known whether it is implicated in the targeting of proteins to the proteasome for degradation. Is able to form polymeric chains with itself at Lys-58, similar to ubiquitin and other ubiquitin-like proteins. Ref.2

Ontologies

Keywords
   Biological processUbl conjugation pathway
   PTMIsopeptide bond
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein modification by small protein conjugation

Inferred from direct assay Ref.2. Source: UniProtKB

   Molecular_functionprotein tag

Inferred from direct assay Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6666Small archaeal modifier protein 2
PRO_0000397102

Amino acid modifications

Cross-link58Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SAMP2) Ref.2
Cross-link66Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Experimental info

Mutagenesis65 – 662Missing: Abolishes SAMPylation of substrate proteins. Ref.2

Secondary structure

................. 66
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
D4GZE7 [UniParc].

Last modified May 18, 2010. Version 1.
Checksum: 09442B0B845C7DE0

FASTA667,118
        10         20         30         40         50         60 
MNVTVEVVGE ETSEVAVDDD GTYADLVRAV DLSPHEVTVL VDGRPVPEDQ SVEVDRVKVL 


RLIKGG 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Haloferax volcanii DS2, a model archaeon."
Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.
PLoS ONE 5:E9605-E9605(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
[2]"Ubiquitin-like small archaeal modifier proteins (SAMPs) in Haloferax volcanii."
Humbard M.A., Miranda H.V., Lim J.M., Krause D.J., Pritz J.R., Zhou G., Chen S., Wells L., Maupin-Furlow J.A.
Nature 463:54-60(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A PROTEIN MODIFIER, CROSS-LINK, PROTEIN TARGETS, SAMPYLATION AT LYS-58, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF 65-GLY-GLY-66.
[3]"SAMPyling proteins in archaea."
Darwin K.H., Hofmann K.
Trends Biochem. Sci. 35:348-351(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001956 Genomic DNA. Translation: ADE03392.1.
RefSeqYP_003534279.1. NC_013967.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2L32NMR-A1-66[»]
2LJINMR-A1-66[»]
4HRSX-ray2.30A2-66[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE03392; ADE03392; HVO_0202.
GeneID8926718.
KEGGhvo:HVO_0202.

Phylogenomic databases

HOGENOMHOG000227894.
KOK03154.
OMAPEDQPVE.

Enzyme and pathway databases

BioCycHVOL309800:GCOK-206-MONOMER.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR012675. Beta-grasp_dom.
IPR016155. Mopterin_synth/thiamin_S_b.
[Graphical view]
SUPFAMSSF54285. SSF54285. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceD4GZE7.

Entry information

Entry nameSAMP2_HALVD
AccessionPrimary (citable) accession number: D4GZE7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: May 18, 2010
Last modified: January 22, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references