D4GZE7 (SAMP2_HALVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small archaeal modifier protein 2
Short name=SAMP 2

Alternative name(s):
Ubiquitin-like small archaeal modifier protein 2

Gene names

Ordered Locus Names:

HVO_0202

Organism

Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Complete proteome] [HAMAP]

Taxonomic identifier

309800 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloferax › Haloferax volcanii

Protein attributes

Sequence length	66 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Protein modifier that is covalently attached to lysine residues of substrate proteins. The tagging system is termed SAMPylation. It is not known whether it is implicated in the targeting of proteins to the proteasome for degradation. Is able to form polymeric chains with itself at Lys-58, similar to ubiquitin and other ubiquitin-like proteins. Ref.2

Ontologies

Keywords
Biological process	Ubl conjugation pathway
PTM	Isopeptide bond Ubl conjugation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	protein modification by small protein conjugation Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	protein tag Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 66

66

Small archaeal modifier protein 2

PRO_0000397102

Amino acid modifications

Cross-link

58

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SAMP2) Ref.2

Cross-link

66

Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Experimental info

Mutagenesis

65 – 66

2

Missing: Abolishes SAMPylation of substrate proteins. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

66

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Beta strand

8 – 16

9

Helix

23 – 28

6

Helix

47 – 49

3

Beta strand

50 – 52

3

Beta strand

57 – 59

3

Sequences

	Sequence		Length	Mass (Da)
	D4GZE7 [UniParc]. Last modified May 18, 2010. Version 1. Checksum: 09442B0B845C7DE0	FASTA	66	7,118
	10 20 30 40 50 60 MNVTVEVVGE ETSEVAVDDD GTYADLVRAV DLSPHEVTVL VDGRPVPEDQ SVEVDRVKVL RLIKGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Haloferax volcanii DS2, a model archaeon." Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A. PLoS ONE 5:E9605-E9605(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
[2]	"Ubiquitin-like small archaeal modifier proteins (SAMPs) in Haloferax volcanii." Humbard M.A., Miranda H.V., Lim J.M., Krause D.J., Pritz J.R., Zhou G., Chen S., Wells L., Maupin-Furlow J.A. Nature 463:54-60(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A PROTEIN MODIFIER, CROSS-LINK, PROTEIN TARGETS, SAMPYLATION AT LYS-58, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF 65-GLY-GLY-66.
[3]	"SAMPyling proteins in archaea." Darwin K.H., Hofmann K. Trends Biochem. Sci. 35:348-351(2010) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP001956 Genomic DNA. Translation: ADE03392.1.

RefSeq

YP_003534279.1. NC_013967.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L32	NMR	-	A	1-66	[»]
2LJI	NMR	-	A	1-66	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

ADE03392; ADE03392; HVO_0202.

GeneID

8926718.

KEGG

hvo:HVO_0202.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HOG000227894.

KO

K03154.

OMA

PEDQPVE.

Family and domain databases

Gene3D

3.10.20.30. 1 hit.

InterPro

IPR012675. Beta-grasp_dom.
IPR016155. Mopterin_synth/thiamin_S_b.
[Graphical view]

SUPFAM

SSF54285. Mo_synth/thiamin_syn_S_b-grasp. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

D4GZE7.

Entry information

Entry name

SAMP2_HALVD

Accession

Primary (citable) accession number: D4GZE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program