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D4GYZ1

- PSB_HALVD

UniProt

D4GYZ1 - PSB_HALVD

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Protein
Proteasome subunit beta
Gene
psmB, HVO_1562
Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The H.volcanii alpha1-beta proteasome is able to cleave oligopeptides after Phe, Tyr and Trp, poorly after Glu but not after Arg. Thus, displays chymotrypsin-like activity, low caspase-like activity but no trypsin-like activity.1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.1 Publication

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. In vitro, the chymotrypsin-like activity of the alpha1-beta proteasome is potently inhibited by carbobenzoxyl-leucinyl-leucinyl-leucinal-H (MG132) and significantly by N-acetyl-leucinyl-leucinyl-norleucinal-H (calpain inhibitor I).1 Publication

pH dependencei

Optimum pH is 7.0-9.3 for the Suc-LLVY-Amc hydrolyzing activity (with the alpha1-beta proteasome subtype).1 Publication

Temperature dependencei

Optimum temperature is 75 degrees Celsius for the Suc-LLVY-Amc hydrolyzing activity (with the alpha1-beta proteasome subtype).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei50 – 501Nucleophile By similarity

GO - Molecular functioni

  1. endopeptidase activity Source: UniProtKB
  2. threonine-type endopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. proteasomal protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciHVOL309800:GCOK-1565-MONOMER.

Protein family/group databases

MEROPSiT01.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit
Proteasome core protein PsmB
Gene namesi
Name:psmB
Ordered Locus Names:HVO_1562
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
ProteomesiUP000008243: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. proteasome core complex, beta-subunit complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

Pathology & Biotechi

Disruption phenotypei

Strains lacking psmB gene are inviable.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 4949Removed in mature form; by autocatalysisUniRule annotation
PRO_0000397605Add
BLAST
Chaini50 – 243194Proteasome subunit betaUniRule annotation
PRO_0000397606Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei129 – 1291Phosphoserine1 Publication

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Zymogen

PTM databases

PhosSiteiP0611175.

Expressioni

Inductioni

Up-regulated at the mRNA level during transition from exponential to stationary phase. However, at the protein level, PsmB is expressed at a high and relatively constant level throughout growth.2 Publications

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. H.volcanii produces at least 2 types of 20S proteasomes: an alpha1-beta proteasome and a proteasome containing all three subunits (alpha1, alpha2, and beta) that appears to be asymmetrical with homo-oligomeric alpha1 and alpha2 rings positioned on separate ends. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped at one or both ends by the proteasome regulatory ATPase, PAN.2 Publications

Structurei

3D structure databases

ProteinModelPortaliD4GYZ1.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.

Phylogenomic databases

HOGENOMiHOG000091083.
KOiK03433.
OMAiMVDWLGT.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_A. Proteasome_B_A.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019983. Pept_T1A_Psome_bsu_arc.
IPR000243. Pept_T1A_subB.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03634. arc_protsome_B. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D4GYZ1-1 [UniParc]FASTAAdd to Basket

« Hide

MRTPTHDEFS GRLDSLNGDR SNVFGPELGE FSNADRRADE LGDKETKTGT    50
TTVGIKTEEG VVLATDMRAS MGYMVSSKDV QKVEEIHPTG ALTIAGSVSA 100
AQSLISSLRA EVRLYEARRG EDMSMQALST LVGNFLRSGG FYVVQPILGG 150
VDETGPHIYS IDPAGSILEE EYTVTGSGSQ YALGVLEQEF EDGLSIEEAK 200
GVATKAIRSA VERDLASGNG INIAVVTEDG VDIQRHQNFE GLE 243
Length:243
Mass (Da):25,994
Last modified:May 18, 2010 - v1
Checksum:i6253E28ADDA40E61
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF126262 Genomic DNA. Translation: AAD53406.1.
CP001956 Genomic DNA. Translation: ADE03141.1.
PIRiT48677.
RefSeqiYP_003535609.1. NC_013967.1.

Genome annotation databases

EnsemblBacteriaiADE03141; ADE03141; HVO_1562.
GeneIDi8923965.
KEGGihvo:HVO_1562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF126262 Genomic DNA. Translation: AAD53406.1 .
CP001956 Genomic DNA. Translation: ADE03141.1 .
PIRi T48677.
RefSeqi YP_003535609.1. NC_013967.1.

3D structure databases

ProteinModelPortali D4GYZ1.
ModBasei Search...

Protein family/group databases

MEROPSi T01.002.

PTM databases

PhosSitei P0611175.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADE03141 ; ADE03141 ; HVO_1562 .
GeneIDi 8923965.
KEGGi hvo:HVO_1562.

Phylogenomic databases

HOGENOMi HOG000091083.
KOi K03433.
OMAi MVDWLGT.

Enzyme and pathway databases

BioCyci HVOL309800:GCOK-1565-MONOMER.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_02113_A. Proteasome_B_A.
InterProi IPR029055. Ntn_hydrolases_N.
IPR019983. Pept_T1A_Psome_bsu_arc.
IPR000243. Pept_T1A_subB.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR03634. arc_protsome_B. 1 hit.
PROSITEi PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Halophilic 20S proteasomes of the archaeon Haloferax volcanii: purification, characterization, and gene sequence analysis."
    Wilson H.L., Aldrich H.C., Maupin-Furlow J.
    J. Bacteriol. 181:5814-5824(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 50-79, FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SALT REQUIREMENT, SUBUNIT.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  3. "Subunit topology of two 20S proteasomes from Haloferax volcanii."
    Kaczowka S.J., Maupin-Furlow J.A.
    J. Bacteriol. 185:165-174(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  4. "Differential regulation of the PanA and PanB proteasome-activating nucleotidase and 20S proteasomal proteins of the haloarchaeon Haloferax volcanii."
    Reuter C.J., Kaczowka S.J., Maupin-Furlow J.A.
    J. Bacteriol. 186:7763-7772(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Posttranslational modification of the 20S proteasomal proteins of the archaeon Haloferax volcanii."
    Humbard M.A., Stevens S.M. Jr., Maupin-Furlow J.A.
    J. Bacteriol. 188:7521-7530(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-129.
  6. "Proteasomal components required for cell growth and stress responses in the haloarchaeon Haloferax volcanii."
    Zhou G., Kowalczyk D., Humbard M.A., Rohatgi S., Maupin-Furlow J.A.
    J. Bacteriol. 190:8096-8105(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPSB_HALVD
AccessioniPrimary (citable) accession number: D4GYZ1
Secondary accession number(s): Q9V2V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 18, 2010
Last modified: June 11, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

H.volcanii proteasome requires high concentrations of salt, similar to the extracellular environment and cytoplasm of this organism, for complex stability and optimal activity.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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