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D4GYH5

- AGLM_HALVD

UniProt

D4GYH5 - AGLM_HALVD

Protein

UDP-glucose 6-dehydrogenase AglM

Gene

aglM

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (18 May 2010)
      Previous versions | rss
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    Functioni

    Involved in the assembly of a N-linked pentasaccharide that decorates the S-layer glycoprotein and flagellins. Involved in the biosynthesis of the hexuronic acids found at both positions 2 and 3 of the pentasaccharide.2 Publications

    Catalytic activityi

    UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.1 Publication

    Enzyme regulationi

    Activity improves as salinity decreases.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei269 – 2691By similarity

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. UDP-glucose 6-dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide biosynthetic process Source: InterPro
    2. S-layer organization Source: UniProtKB-UniPathway
    3. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciHVOL309800:GCOK-1534-MONOMER.
    UniPathwayiUPA00038; UER00491.
    UPA00977.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 6-dehydrogenase AglM (EC:1.1.1.22)
    Short name:
    UDP-Glc dehydrogenase
    Short name:
    UDP-GlcDH
    Short name:
    UDPGDH
    Alternative name(s):
    Archaeal glycosylation protein M
    Gene namesi
    Name:aglM
    Ordered Locus Names:HVO_1531
    OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
    Taxonomic identifieri309800 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
    ProteomesiUP000008243: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Mutants exhibit defective or limited motility.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 430430UDP-glucose 6-dehydrogenase AglMPRO_0000415417Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliD4GYH5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000153773.
    KOiK00012.
    OMAiRETNPTA.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028357. UDPglc_DH_bac.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500134. UDPglc_DH_bac. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D4GYH5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELSIIGSGY VGTTIAACFA ELGHDVVNVD IDEDIVASLN DGQAPIHEPG    50
    LAELVERYAG DRLRATTDYD EILDTDATFL ALPTPSTDDG SIDLGAMKTA 100
    ATSLGETLAR KDDSHLVVTK STVVPRTTVD VIGPRIEEAS GKRVGDGLDI 150
    AMNPEFLREG TAVDDFLSPD KIVLGAQTDR AYETLAEIFA PLVERAGNPP 200
    VVKTGISEAE MIKYANNAFL ASKISLANDL ANICKVFGVD SAEVLESIGL 250
    DSRIGSAFLG AGLGWGGSCF PKDTAAIIAA ARAQGYEPRL LQAAVDVNDG 300
    QPERMLDLLR ERFDLDGKRV AVLGLAFKPG TDDIRKSRAI LLIQALLDAG 350
    ADVVGYDPVA TENMRERFPD IDYADSAADA LANADAALVA TDWDEFAALD 400
    DEFDAMRERI VIDGRRIVTR REGLDYESLV 430
    Length:430
    Mass (Da):45,967
    Last modified:May 18, 2010 - v1
    Checksum:iBC8ABB67E71DFF7F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN386609 Genomic DNA. Translation: CAY46591.1.
    CP001956 Genomic DNA. Translation: ADE05103.1.
    RefSeqiWP_004041409.1. NZ_AOHU01000028.1.
    YP_003535578.1. NC_013967.1.

    Genome annotation databases

    EnsemblBacteriaiADE05103; ADE05103; HVO_1531.
    GeneIDi8924832.
    KEGGihvo:HVO_1531.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN386609 Genomic DNA. Translation: CAY46591.1 .
    CP001956 Genomic DNA. Translation: ADE05103.1 .
    RefSeqi WP_004041409.1. NZ_AOHU01000028.1.
    YP_003535578.1. NC_013967.1.

    3D structure databases

    ProteinModelPortali D4GYH5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADE05103 ; ADE05103 ; HVO_1531 .
    GeneIDi 8924832.
    KEGGi hvo:HVO_1531.

    Phylogenomic databases

    HOGENOMi HOG000153773.
    KOi K00012.
    OMAi RETNPTA.

    Enzyme and pathway databases

    UniPathwayi UPA00038 ; UER00491 .
    UPA00977 .
    BioCyci HVOL309800:GCOK-1534-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028357. UDPglc_DH_bac.
    [Graphical view ]
    PANTHERi PTHR11374. PTHR11374. 1 hit.
    Pfami PF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500134. UDPglc_DH_bac. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "N-glycosylation in Archaea: on the coordinated actions of Haloferax volcanii AglF and AglM."
      Yurist-Doutsch S., Magidovich H., Ventura V.V., Hitchen P.G., Dell A., Eichler J.
      Mol. Microbiol. 75:1047-1058(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, PATHWAY, GENE NAME.
      Strain: DS2 / DS70.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
    3. "N-glycosylation of Haloferax volcanii flagellins requires known Agl proteins and is essential for biosynthesis of stable flagella."
      Tripepi M., You J., Temel S., Onder O., Brisson D., Pohlschroder M.
      J. Bacteriol. 194:4876-4887(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN GLYCOSYLATION OF FLAGELLINS, DISRUPTION PHENOTYPE.
      Strain: H53.

    Entry informationi

    Entry nameiAGLM_HALVD
    AccessioniPrimary (citable) accession number: D4GYH5
    Secondary accession number(s): C3W971
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3