D4GYH5 (AGLM_HALVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose 6-dehydrogenase AglM
Short name=UDP-Glc dehydrogenase
Short name=UDP-GlcDH
Short name=UDPGDH
EC=1.1.1.22

Alternative name(s):
Archaeal glycosylation protein M

Gene names

Name:	aglM
Ordered Locus Names:	HVO_1531

Organism

Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Complete proteome] [HAMAP]

Taxonomic identifier

309800 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloferax › Haloferax volcanii

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in N-glycosylation of the S-layer glycoprotein. Contributes to the assembly of the pentasaccharide decorating at least 2 select Asn residues of the S-layer glycoprotein. Involved in the biosynthesis of the hexuronic acids found at both positions 2 and 3 of the pentasaccharide. Ref.1
Catalytic activity	UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH. Ref.1
Enzyme regulation	Activity improves as salinity decreases. Ref.1
Pathway	Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. Ref.1 Cell surface structure biogenesis; S-layer biogenesis. Ref.1
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	S-layer organization Inferred from electronic annotation. Source: UniProtKB-UniPathway UDP-glucuronate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	UDP-glucose 6-dehydrogenase AglM		PRO_0000415417
Sites
	Active site	269	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	D4GYH5 [UniParc]. Last modified May 18, 2010. Version 1. Checksum: BC8ABB67E71DFF7F	FASTA	430	45,967
	10 20 30 40 50 60 MELSIIGSGY VGTTIAACFA ELGHDVVNVD IDEDIVASLN DGQAPIHEPG LAELVERYAG 70 80 90 100 110 120 DRLRATTDYD EILDTDATFL ALPTPSTDDG SIDLGAMKTA ATSLGETLAR KDDSHLVVTK 130 140 150 160 170 180 STVVPRTTVD VIGPRIEEAS GKRVGDGLDI AMNPEFLREG TAVDDFLSPD KIVLGAQTDR 190 200 210 220 230 240 AYETLAEIFA PLVERAGNPP VVKTGISEAE MIKYANNAFL ASKISLANDL ANICKVFGVD 250 260 270 280 290 300 SAEVLESIGL DSRIGSAFLG AGLGWGGSCF PKDTAAIIAA ARAQGYEPRL LQAAVDVNDG 310 320 330 340 350 360 QPERMLDLLR ERFDLDGKRV AVLGLAFKPG TDDIRKSRAI LLIQALLDAG ADVVGYDPVA 370 380 390 400 410 420 TENMRERFPD IDYADSAADA LANADAALVA TDWDEFAALD DEFDAMRERI VIDGRRIVTR 430 REGLDYESLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"N-glycosylation in Archaea: on the coordinated actions of Haloferax volcanii AglF and AglM." Yurist-Doutsch S., Magidovich H., Ventura V.V., Hitchen P.G., Dell A., Eichler J. Mol. Microbiol. 75:1047-1058(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, PATHWAY, GENE NAME. Strain: DS2 / DS70.
[2]	"The complete genome sequence of Haloferax volcanii DS2, a model archaeon." Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A. PLoS ONE 5:E9605-E9605(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN386609 Genomic DNA. Translation: CAY46591.1. CP001956 Genomic DNA. Translation: ADE05103.1.
RefSeq	YP_003535578.1. NC_013967.1.
3D structure databases
ProteinModelPortal	D4GYH5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADE05103; ADE05103; HVO_1531.
GeneID	8924832.
KEGG	hvo:HVO_1531.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000153773.
KO	K00012.
OMA	QMCPEIR.
ProtClustDB	CLSK510967.
Enzyme and pathway databases
UniPathway	UPA00038; UER00491. UPA00977.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DH_C. IPR014026. UDP-Glc/GDP-Man_DH_dimer. IPR001732. UDP-Glc/GDP-Man_DH_N. [Graphical view]
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMART	SM00984. UDPG_MGDP_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit. SSF52413. UDP-Glc/GDP-Man_DH_C. 1 hit.
TIGRFAMs	TIGR03026. NDP-sugDHase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AGLM_HALVD

Accession

Primary (citable) accession number: D4GYH5
Secondary accession number(s): C3W971

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2012
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program