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D4GYH5

- AGLM_HALVD

UniProt

D4GYH5 - AGLM_HALVD

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Protein
UDP-glucose 6-dehydrogenase AglM
Gene
aglM, HVO_1531
Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the assembly of a N-linked pentasaccharide that decorates the S-layer glycoprotein and flagellins. Involved in the biosynthesis of the hexuronic acids found at both positions 2 and 3 of the pentasaccharide.2 Publications

Catalytic activityi

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.1 Publication

Enzyme regulationi

Activity improves as salinity decreases.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei269 – 2691 By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. UDP-glucose 6-dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. S-layer organization Source: UniProtKB-UniPathway
  2. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway
  3. polysaccharide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciHVOL309800:GCOK-1534-MONOMER.
UniPathwayiUPA00038; UER00491.
UPA00977.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 6-dehydrogenase AglM (EC:1.1.1.22)
Short name:
UDP-Glc dehydrogenase
Short name:
UDP-GlcDH
Short name:
UDPGDH
Alternative name(s):
Archaeal glycosylation protein M
Gene namesi
Name:aglM
Ordered Locus Names:HVO_1531
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
ProteomesiUP000008243: Chromosome

Pathology & Biotechi

Disruption phenotypei

Mutants exhibit defective or limited motility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430UDP-glucose 6-dehydrogenase AglM
PRO_0000415417Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliD4GYH5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000153773.
KOiK00012.
OMAiRETNPTA.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028357. UDPglc_DH_bac.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500134. UDPglc_DH_bac. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

D4GYH5-1 [UniParc]FASTAAdd to Basket

« Hide

MELSIIGSGY VGTTIAACFA ELGHDVVNVD IDEDIVASLN DGQAPIHEPG    50
LAELVERYAG DRLRATTDYD EILDTDATFL ALPTPSTDDG SIDLGAMKTA 100
ATSLGETLAR KDDSHLVVTK STVVPRTTVD VIGPRIEEAS GKRVGDGLDI 150
AMNPEFLREG TAVDDFLSPD KIVLGAQTDR AYETLAEIFA PLVERAGNPP 200
VVKTGISEAE MIKYANNAFL ASKISLANDL ANICKVFGVD SAEVLESIGL 250
DSRIGSAFLG AGLGWGGSCF PKDTAAIIAA ARAQGYEPRL LQAAVDVNDG 300
QPERMLDLLR ERFDLDGKRV AVLGLAFKPG TDDIRKSRAI LLIQALLDAG 350
ADVVGYDPVA TENMRERFPD IDYADSAADA LANADAALVA TDWDEFAALD 400
DEFDAMRERI VIDGRRIVTR REGLDYESLV 430
Length:430
Mass (Da):45,967
Last modified:May 18, 2010 - v1
Checksum:iBC8ABB67E71DFF7F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN386609 Genomic DNA. Translation: CAY46591.1.
CP001956 Genomic DNA. Translation: ADE05103.1.
RefSeqiWP_004041409.1. NZ_AOHU01000028.1.
YP_003535578.1. NC_013967.1.

Genome annotation databases

EnsemblBacteriaiADE05103; ADE05103; HVO_1531.
GeneIDi8924832.
KEGGihvo:HVO_1531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN386609 Genomic DNA. Translation: CAY46591.1 .
CP001956 Genomic DNA. Translation: ADE05103.1 .
RefSeqi WP_004041409.1. NZ_AOHU01000028.1.
YP_003535578.1. NC_013967.1.

3D structure databases

ProteinModelPortali D4GYH5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADE05103 ; ADE05103 ; HVO_1531 .
GeneIDi 8924832.
KEGGi hvo:HVO_1531.

Phylogenomic databases

HOGENOMi HOG000153773.
KOi K00012.
OMAi RETNPTA.

Enzyme and pathway databases

UniPathwayi UPA00038 ; UER00491 .
UPA00977 .
BioCyci HVOL309800:GCOK-1534-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028357. UDPglc_DH_bac.
[Graphical view ]
PANTHERi PTHR11374. PTHR11374. 1 hit.
Pfami PF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF500134. UDPglc_DH_bac. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "N-glycosylation in Archaea: on the coordinated actions of Haloferax volcanii AglF and AglM."
    Yurist-Doutsch S., Magidovich H., Ventura V.V., Hitchen P.G., Dell A., Eichler J.
    Mol. Microbiol. 75:1047-1058(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, PATHWAY, GENE NAME.
    Strain: DS2 / DS70.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  3. "N-glycosylation of Haloferax volcanii flagellins requires known Agl proteins and is essential for biosynthesis of stable flagella."
    Tripepi M., You J., Temel S., Onder O., Brisson D., Pohlschroder M.
    J. Bacteriol. 194:4876-4887(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GLYCOSYLATION OF FLAGELLINS, DISRUPTION PHENOTYPE.
    Strain: H53.

Entry informationi

Entry nameiAGLM_HALVD
AccessioniPrimary (citable) accession number: D4GYH5
Secondary accession number(s): C3W971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: May 18, 2010
Last modified: September 3, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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