D4GYH1 (AGLF_HALVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UTP--glucose-1-phosphate uridylyltransferase AglF
EC=2.7.7.9

Alternative name(s):
Archaeal glycosylation protein F

Gene names

Name:	aglF
Ordered Locus Names:	HVO_1527

Organism

Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Complete proteome] [HAMAP]

Taxonomic identifier

309800 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloferax › Haloferax volcanii

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in N-glycosylation of the S-layer glycoprotein. Contributes to the assembly of the pentasaccharide decorating at least 2 select Asn residues of the S-layer glycoprotein. Involved in the addition of the hexuronic acid found at position 3 of the pentasaccharide. Ref.1 Ref.3
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose. Ref.3
Pathway	Cell surface structure biogenesis; S-layer biogenesis. Ref.3
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	S-layer organization Inferred from electronic annotation. Source: UniProtKB-UniPathway biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	UTP--glucose-1-phosphate uridylyltransferase AglF		PRO_0000415423

Sequences

	Sequence		Length	Mass (Da)
	D4GYH1 [UniParc]. Last modified May 18, 2010. Version 1. Checksum: 6AA3AC63E053AA35	FASTA	243	27,535
	10 20 30 40 50 60 MQAVVLAAGK GTRLRPLTED KPKGMVEVDG KPILTHCFDQ LVDLGAEKLV VVVGYKKEII 70 80 90 100 110 120 IQHYDDEYRG VPITYAHQRE QKGLAHALLT VEDHIDEDFM LMLGDNIFNA NLGDVVKRQR 130 140 150 160 170 180 EDRADAAFLV EEVDWDEASR YGVCVTNDYG EITEVIEKPE EPPSNLVMTG FYTFTPAIFH 190 200 210 220 230 240 ACHLVQPSNR GEYEISEAID LLIRSGRTID AIRIDGWRLD IGYPEDRDEA EQRLQEETTQ ATE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AglF, aglG and aglI, novel members of a gene island involved in the N-glycosylation of the Haloferax volcanii S-layer glycoprotein." Yurist-Doutsch S., Abu-Qarn M., Battaglia F., Morris H.R., Hitchen P.G., Dell A., Eichler J. Mol. Microbiol. 69:1234-1245(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN GLYCOSYLATION, GENE NAME. Strain: DS2 / DS70.
[2]	"The complete genome sequence of Haloferax volcanii DS2, a model archaeon." Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A. PLoS ONE 5:E9605-E9605(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
[3]	"N-glycosylation in Archaea: on the coordinated actions of Haloferax volcanii AglF and AglM." Yurist-Doutsch S., Magidovich H., Ventura V.V., Hitchen P.G., Dell A., Eichler J. Mol. Microbiol. 75:1047-1058(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY. Strain: DS2 / DS70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM991128 Genomic DNA. Translation: CAQ51229.1. CP001956 Genomic DNA. Translation: ADE04323.1.
RefSeq	YP_003535574.1. NC_013967.1.
3D structure databases
ProteinModelPortal	D4GYH1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADE04323; ADE04323; HVO_1527.
GeneID	8924609.
KEGG	hvo:HVO_1527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000283477.
KO	K00973.
OMA	IEQILIV.
Enzyme and pathway databases
UniPathway	UPA00977.
Family and domain databases
InterPro	IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGLF_HALVD

Accession

Primary (citable) accession number: D4GYH1
Secondary accession number(s): B2G4W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2012
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program