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Protein

UTP--glucose-1-phosphate uridylyltransferase AglF

Gene

aglF

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the assembly of a N-linked pentasaccharide that decorates the S-layer glycoprotein and flagellins. Involved in the biosynthesis of the hexuronic acid found at position 3 of the pentasaccharide.3 Publications

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.1 Publication

Pathwayi

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciHVOL309800:GCOK-1530-MONOMER.
UniPathwayiUPA00977.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase AglF (EC:2.7.7.9)
Alternative name(s):
Archaeal glycosylation protein F
Gene namesi
Name:aglF
Ordered Locus Names:HVO_1527
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
ProteomesiUP000008243 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Mutants exhibit defective or limited motility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243UTP--glucose-1-phosphate uridylyltransferase AglFPRO_0000415423Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliD4GYH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDPGP type 2 family.Curated

Phylogenomic databases

HOGENOMiHOG000283477.
KOiK00973.
OMAiDEYEGVP.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

D4GYH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAVVLAAGK GTRLRPLTED KPKGMVEVDG KPILTHCFDQ LVDLGAEKLV
60 70 80 90 100
VVVGYKKEII IQHYDDEYRG VPITYAHQRE QKGLAHALLT VEDHIDEDFM
110 120 130 140 150
LMLGDNIFNA NLGDVVKRQR EDRADAAFLV EEVDWDEASR YGVCVTNDYG
160 170 180 190 200
EITEVIEKPE EPPSNLVMTG FYTFTPAIFH ACHLVQPSNR GEYEISEAID
210 220 230 240
LLIRSGRTID AIRIDGWRLD IGYPEDRDEA EQRLQEETTQ ATE
Length:243
Mass (Da):27,535
Last modified:May 18, 2010 - v1
Checksum:i6AA3AC63E053AA35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM991128 Genomic DNA. Translation: CAQ51229.1.
CP001956 Genomic DNA. Translation: ADE04323.1.
RefSeqiWP_004041405.1. NZ_AOHU01000028.1.
YP_003535574.1. NC_013967.1.

Genome annotation databases

EnsemblBacteriaiADE04323; ADE04323; HVO_1527.
GeneIDi8924609.
KEGGihvo:HVO_1527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM991128 Genomic DNA. Translation: CAQ51229.1.
CP001956 Genomic DNA. Translation: ADE04323.1.
RefSeqiWP_004041405.1. NZ_AOHU01000028.1.
YP_003535574.1. NC_013967.1.

3D structure databases

ProteinModelPortaliD4GYH1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE04323; ADE04323; HVO_1527.
GeneIDi8924609.
KEGGihvo:HVO_1527.

Phylogenomic databases

HOGENOMiHOG000283477.
KOiK00973.
OMAiDEYEGVP.

Enzyme and pathway databases

UniPathwayiUPA00977.
BioCyciHVOL309800:GCOK-1530-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "AglF, aglG and aglI, novel members of a gene island involved in the N-glycosylation of the Haloferax volcanii S-layer glycoprotein."
    Yurist-Doutsch S., Abu-Qarn M., Battaglia F., Morris H.R., Hitchen P.G., Dell A., Eichler J.
    Mol. Microbiol. 69:1234-1245(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN GLYCOSYLATION, GENE NAME.
    Strain: DS2 / DS70.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  3. "N-glycosylation in Archaea: on the coordinated actions of Haloferax volcanii AglF and AglM."
    Yurist-Doutsch S., Magidovich H., Ventura V.V., Hitchen P.G., Dell A., Eichler J.
    Mol. Microbiol. 75:1047-1058(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
    Strain: DS2 / DS70.
  4. "N-glycosylation of Haloferax volcanii flagellins requires known Agl proteins and is essential for biosynthesis of stable flagella."
    Tripepi M., You J., Temel S., Onder O., Brisson D., Pohlschroder M.
    J. Bacteriol. 194:4876-4887(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GLYCOSYLATION OF FLAGELLINS, DISRUPTION PHENOTYPE.
    Strain: H53.

Entry informationi

Entry nameiAGLF_HALVD
AccessioniPrimary (citable) accession number: D4GYH1
Secondary accession number(s): B2G4W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: May 18, 2010
Last modified: January 7, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.