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D4GYH1

- AGLF_HALVD

UniProt

D4GYH1 - AGLF_HALVD

Protein

UTP--glucose-1-phosphate uridylyltransferase AglF

Gene

aglF

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (18 May 2010)
      Previous versions | rss
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    Functioni

    Involved in the assembly of a N-linked pentasaccharide that decorates the S-layer glycoprotein and flagellins. Involved in the biosynthesis of the hexuronic acid found at position 3 of the pentasaccharide.3 Publications

    Catalytic activityi

    UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. UTP:glucose-1-phosphate uridylyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. S-layer organization Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciHVOL309800:GCOK-1530-MONOMER.
    UniPathwayiUPA00977.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase AglF (EC:2.7.7.9)
    Alternative name(s):
    Archaeal glycosylation protein F
    Gene namesi
    Name:aglF
    Ordered Locus Names:HVO_1527
    OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
    Taxonomic identifieri309800 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
    ProteomesiUP000008243: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Mutants exhibit defective or limited motility.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 243243UTP--glucose-1-phosphate uridylyltransferase AglFPRO_0000415423Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliD4GYH1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDPGP type 2 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000283477.
    KOiK00973.
    OMAiILTHCFD.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF00483. NTP_transferase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D4GYH1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAVVLAAGK GTRLRPLTED KPKGMVEVDG KPILTHCFDQ LVDLGAEKLV    50
    VVVGYKKEII IQHYDDEYRG VPITYAHQRE QKGLAHALLT VEDHIDEDFM 100
    LMLGDNIFNA NLGDVVKRQR EDRADAAFLV EEVDWDEASR YGVCVTNDYG 150
    EITEVIEKPE EPPSNLVMTG FYTFTPAIFH ACHLVQPSNR GEYEISEAID 200
    LLIRSGRTID AIRIDGWRLD IGYPEDRDEA EQRLQEETTQ ATE 243
    Length:243
    Mass (Da):27,535
    Last modified:May 18, 2010 - v1
    Checksum:i6AA3AC63E053AA35
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM991128 Genomic DNA. Translation: CAQ51229.1.
    CP001956 Genomic DNA. Translation: ADE04323.1.
    RefSeqiWP_004041405.1. NZ_AOHU01000028.1.
    YP_003535574.1. NC_013967.1.

    Genome annotation databases

    EnsemblBacteriaiADE04323; ADE04323; HVO_1527.
    GeneIDi8924609.
    KEGGihvo:HVO_1527.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM991128 Genomic DNA. Translation: CAQ51229.1 .
    CP001956 Genomic DNA. Translation: ADE04323.1 .
    RefSeqi WP_004041405.1. NZ_AOHU01000028.1.
    YP_003535574.1. NC_013967.1.

    3D structure databases

    ProteinModelPortali D4GYH1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADE04323 ; ADE04323 ; HVO_1527 .
    GeneIDi 8924609.
    KEGGi hvo:HVO_1527.

    Phylogenomic databases

    HOGENOMi HOG000283477.
    KOi K00973.
    OMAi ILTHCFD.

    Enzyme and pathway databases

    UniPathwayi UPA00977 .
    BioCyci HVOL309800:GCOK-1530-MONOMER.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF00483. NTP_transferase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "AglF, aglG and aglI, novel members of a gene island involved in the N-glycosylation of the Haloferax volcanii S-layer glycoprotein."
      Yurist-Doutsch S., Abu-Qarn M., Battaglia F., Morris H.R., Hitchen P.G., Dell A., Eichler J.
      Mol. Microbiol. 69:1234-1245(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN GLYCOSYLATION, GENE NAME.
      Strain: DS2 / DS70.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
    3. "N-glycosylation in Archaea: on the coordinated actions of Haloferax volcanii AglF and AglM."
      Yurist-Doutsch S., Magidovich H., Ventura V.V., Hitchen P.G., Dell A., Eichler J.
      Mol. Microbiol. 75:1047-1058(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
      Strain: DS2 / DS70.
    4. "N-glycosylation of Haloferax volcanii flagellins requires known Agl proteins and is essential for biosynthesis of stable flagella."
      Tripepi M., You J., Temel S., Onder O., Brisson D., Pohlschroder M.
      J. Bacteriol. 194:4876-4887(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN GLYCOSYLATION OF FLAGELLINS, DISRUPTION PHENOTYPE.
      Strain: H53.

    Entry informationi

    Entry nameiAGLF_HALVD
    AccessioniPrimary (citable) accession number: D4GYH1
    Secondary accession number(s): B2G4W5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3