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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei150Proton acceptorUniRule annotation1
Binding sitei152SubstrateUniRule annotation1
Metal bindingi176Magnesium; via carbamate groupUniRule annotation1
Metal bindingi178MagnesiumUniRule annotation1
Metal bindingi179MagnesiumUniRule annotation1
Active sitei268Proton acceptorUniRule annotation1
Binding sitei269SubstrateUniRule annotation1
Binding sitei301SubstrateUniRule annotation1
Sitei309Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotationImported
Ordered Locus Names:HVO_0970Imported
ORF Names:C498_13921Imported
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)Imported
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloferax
Proteomesi
  • UP000011532 Componenti: Unassembled WGS sequence
  • UP000008243 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei176N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi309800.HVO_0970.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 118RuBisCO_large_NInterPro annotationAdd BLAST112
Domaini133 – 408RuBisCO_largeInterPro annotationAdd BLAST276

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni337 – 339Substrate bindingUniRule annotation3
Regioni359 – 362Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

D4GV77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGITYEDFLD LDYEPTDEDL VCTFRIDPAT GMSTEAAASR VASESSNGTW
60 70 80 90 100
AALQTGADFT DMGATTFDID GDLIRVAYPA GLFEPGNMPQ VLSCIAGNIM
110 120 130 140 150
GMKAVDTIRL LDCEWPESVV SAYPGPLYGS SVREEIFGVT DRPITATVPK
160 170 180 190 200
PKVGLSTAAH AQVGYDAWVG GVDLLKDDEN LTDQAFNPFS DRLTESLSLR
210 220 230 240 250
DDAEDETGEK KSYLINVTAD TQTMLDRVDE VAAQGGEYVM VDIITAGWAG
260 270 280 290 300
LQTVRERTEK HGLAIHAHRA MHAAFDRLPA HGVSMRVLAQ VSRLCGVDQL
310 320 330 340 350
HTGTAGLGKL ANEDTVGINE WLAGDLHGAT DVLPVASGGL HPGLLPDLLD
360 370 380 390 400
ATGTNVCVQL GGGIHGHPDG TRAGAVALRS AIDAYVEGRA ITEAAEETPE
410
LAVALDKWGT ETPR
Length:414
Mass (Da):43,922
Last modified:May 18, 2010 - v1
Checksum:i436AC5738813777B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001956 Genomic DNA. Translation: ADE04153.1.
AOHU01000093 Genomic DNA. Translation: ELY27227.1.
RefSeqiWP_004043974.1. NZ_AOHU01000093.1.

Genome annotation databases

EnsemblBacteriaiADE04153; ADE04153; HVO_0970.
ELY27227; ELY27227; C498_13921.
GeneIDi8926563.
KEGGihvo:HVO_0970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001956 Genomic DNA. Translation: ADE04153.1.
AOHU01000093 Genomic DNA. Translation: ELY27227.1.
RefSeqiWP_004043974.1. NZ_AOHU01000093.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309800.HVO_0970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE04153; ADE04153; HVO_0970.
ELY27227; ELY27227; C498_13921.
GeneIDi8926563.
KEGGihvo:HVO_0970.

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiD4GV77_HALVD
AccessioniPrimary (citable) accession number: D4GV77
Entry historyi
Integrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.