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D4GQU6 (GLPA2_HALVD) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit A2

Short name=G-3-P dehydrogenase A2
Short name=G3PDH A2
EC=1.1.5.3
Gene names
Name:gpdA2
Ordered Locus Names:HVO_A0269
Encoded onPlasmid pHV4
OrganismHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Reference proteome] [HAMAP]
Taxonomic identifier309800 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conversion of glycerol 3-phosphate to dihydroxyacetone phosphate. Functional analog of glpA1 required for the basal levels of glycerol-3-phosphate dehydrogenase activity, but not expressed during standard growth on glycerol or when glpA1 is knockout. Ref.2

Catalytic activity

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol. Ref.2

Cofactor

FAD By similarity.

FMN By similarity.

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.

Subunit structure

Composed of a catalytic GlpA/B dimer and of membrane bound GlpC By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Disruption phenotype

No visible phenotype. Ref.2

Miscellaneous

H.volcanii contains 2 glpABC operons, one located on the main chromosome and the other on megaplasmid pHV4 (Ref.2).

Sequence similarities

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 563563Anaerobic glycerol-3-phosphate dehydrogenase subunit A2
PRO_0000428860

Regions

Nucleotide binding4 – 3229FAD Potential

Sequences

Sequence LengthMass (Da)Tools
D4GQU6 [UniParc].

Last modified May 18, 2010. Version 1.
Checksum: 50F390554781ED4C

FASTA56360,955
        10         20         30         40         50         60 
MSYSVVVIGG GATGTGTARD LAMRGFDVTL VERGNLTEGT TGRTHGHLHS GARYAVSDKE 

        70         80         90        100        110        120 
SAVDCMRENR VLHRIAGHCI EDTGGLFVQL EGDSDDYFER KLAGCAECDI PTEVISGEEA 

       130        140        150        160        170        180 
RRREPYLTDA VERAIWVPDG AVDPFRLCVA NAASAVEHGA RIETHAEVVD LVVEGGRVAG 

       190        200        210        220        230        240 
VEVKRQGPNH HSEGAAGDTE TFEADYVVSA TGAWAGQLAA MAGVDLEMAI SKGAMVVTNV 

       250        260        270        280        290        300 
RQLDTVINRC LPKGEGDTII PHETTVLLGA NDDPVDDPDD YPEEQWEVDM MIDIASEMVP 

       310        320        330        340        350        360 
VVADARMIRA YWGVRPLYDP NPKSTTDPGD VTRNYFVLDH AERDGVAGFA SVVGGKLTTY 

       370        380        390        400        410        420 
REMAESVSDH VCEVLGVEEP CRTDEVPLPG SADPSALDEY MDEFDLRSPI ARRSGQRLGD 

       430        440        450        460        470        480 
RAPEVLDIDE PNPTLCECEA VTRAEVRDAI DQVGADLNGV RLRTRASMGN CQGGFCSHRL 

       490        500        510        520        530        540 
GAELYPDHGA EVARDAVDEL YQERWKGQRH ALWGEQLSQA MLNAMLHATT MNHDANHVAG 

       550        560 
DENIEYRAFD GGRTAVPEGS HGD 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Haloferax volcanii DS2, a model archaeon."
Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.
PLoS ONE 5:E9605-E9605(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
[2]"Activity and transcriptional regulation of bacterial protein-like glycerol-3-phosphate dehydrogenase of the haloarchaea in Haloferax volcanii."
Rawls K.S., Martin J.H., Maupin-Furlow J.A.
J. Bacteriol. 193:4469-4476(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
Strain: DS2 / DS70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001955 Genomic DNA. Translation: ADE02027.1.
RefSeqYP_003533725.1. NC_013966.1.

3D structure databases

ProteinModelPortalD4GQU6.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE02027; ADE02027; HVO_A0269.
GeneID8923720.
KEGGhvo:HVO_A0269.

Phylogenomic databases

HOGENOMHOG000004814.
KOK00111.
OMAWRELEWS.

Enzyme and pathway databases

BioCycHVOL309800:GCOK-3291-MONOMER.
UniPathwayUPA00618; UER00673.

Family and domain databases

InterProIPR007419. BFD-like_2Fe2S-bd_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
IPR017752. G3P_DH_GlpA_su.
[Graphical view]
PfamPF01266. DAO. 1 hit.
PF04324. Fer2_BFD. 1 hit.
[Graphical view]
PRINTSPR01001. FADG3PDH.
TIGRFAMsTIGR03377. glycerol3P_GlpA. 1 hit.
PROSITEPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPA2_HALVD
AccessionPrimary (citable) accession number: D4GQU6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 14, 2014
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways