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D4GPK6

- LACC_HALVD

UniProt

D4GPK6 - LACC_HALVD

Protein

Laccase

Gene

lccA

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (18 May 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of a wide variety of organic substrates, including bilirubin, syringaldazine (SGZ), 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) and dimethoxyphenol (DMP). No oxidation of Fe2+ or guaiacol.1 Publication

    Catalytic activityi

    4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

    Cofactori

    Binds 4 copper ions per subunit.Curated

    Enzyme regulationi

    Inhibited by 1 mM NaN3, 10 mM thiourea, 10 mM 1,10-phenanthroline, 0.1 mM DL-dithiothreitol (DTT) and 1 mM L-cysteine. The inhibition by DTT and L-cysteine is likely caused by reduction of the oxidized substrate and not by inhibition of the enzyme.1 Publication

    Absorptioni

    Abs(max)=605 nm1 Publication

    Kineticsi

    Kcat is 9.9 sec(-1) with 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid as substrate. Kcat is 21.7 sec(-1) with syringaldazine as substrate. Optimal activity at 200 mM salt, with 65% activity at 1 M NaCl.

    1. KM=671 µM for 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid1 Publication
    2. KM=35 µM for syringaldazine1 Publication

    pH dependencei

    Optimum pH is 6.0 for the oxidation of 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid and 8.4 for the oxidation of syringaldazine.1 Publication

    Temperature dependencei

    Optimum temperature is 45-50 degrees Celsius. Highly thermostable.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi145 – 1451Copper 1; type 2By similarity
    Metal bindingi147 – 1471Copper 2; type 3By similarity
    Metal bindingi192 – 1921Copper 2; type 3By similarity
    Metal bindingi194 – 1941Copper 3; type 3By similarity
    Metal bindingi455 – 4551Copper 4; type 1By similarity
    Metal bindingi458 – 4581Copper 1; type 2By similarity
    Metal bindingi460 – 4601Copper 3; type 3By similarity
    Metal bindingi512 – 5121Copper 3; type 3By similarity
    Metal bindingi513 – 5131Copper 4; type 1By similarity
    Metal bindingi514 – 5141Copper 2; type 3By similarity
    Metal bindingi518 – 5181Copper 4; type 1By similarity
    Metal bindingi523 – 5231Copper 4; type 1By similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    BioCyciHVOL309800:GCOK-3862-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase (EC:1.10.3.2)
    Alternative name(s):
    LccA multicopper oxidase
    Gene namesi
    Name:lccA
    Ordered Locus Names:HVO_B0205
    Encoded oniPlasmid pHV30 Publication
    OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
    Taxonomic identifieri309800 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
    ProteomesiUP000008243: Plasmid pHV3

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Chaini32 – 579548LaccasePRO_0000428861Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Exported by the Tat system.
    Glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini82 – 214133Plastocyanin-like 1Add
    BLAST
    Domaini423 – 530108Plastocyanin-like 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 2 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOGENOMiHOG000096435.
    OMAiTYYEVTM.

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    D4GPK6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDWSRRRFL QTGAALGIAG TLPQTTTEVS AASPTLEKFV QPLPIPSVRE    50
    PDGQRDGADA YEIAVTEFTQ QLHPDLPETT VWGFDGSYPG PTIEADAGSP 100
    VHVRFDNSGL PSEHLFPVDD RLGGTTAENH PGYDGPVPEV RTVTHFHGLE 150
    LDPANDGQSD MWTSPGGVEG PRFDSAWQEL PMEQGRTTST YHDHTLGITR 200
    LNAYAGLLGL YSITTDAERE LGLPSGDYDI PLLLQDKEFN DDGSLHYPEE 250
    FVSAFLGDTA VVNGAVWPYV EVEPRRYRFR ILNGANHRSF DLQLESESGS 300
    GVPTMYQFAP GHGFLESVVP IGPNGDLDSL LLTPFERGEL VVDFSDHAGE 350
    TLTLANGADM GPELTDLVEF RVSDPSTPPE DASADPTSLS LPTPASYDES 400
    DARVTREMTL GTEVRNGLIT HTLNGHVFGD EDAPVYPQLG ATEIWELQNE 450
    SGGRHPIHLH LVTFRVIGRG PDGTQPPDPN ELGPKDTVRV DPGERVRILV 500
    TFEGYTGQFP WHCHMLEHED NKMMIPFVVE NPVADYANEE NVVDATGLTD 550
    AVGDWRNETL ETEVLLEVID QWRSGDEVA 579
    Length:579
    Mass (Da):63,442
    Last modified:May 18, 2010 - v1
    Checksum:i567DF329448F0C06
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001953 Genomic DNA. Translation: ADE01515.1.
    RefSeqiYP_003533285.1. NC_013964.1.

    Genome annotation databases

    EnsemblBacteriaiADE01515; ADE01515; HVO_B0205.
    GeneIDi8919198.
    KEGGihvo:HVO_B0205.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001953 Genomic DNA. Translation: ADE01515.1 .
    RefSeqi YP_003533285.1. NC_013964.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADE01515 ; ADE01515 ; HVO_B0205 .
    GeneIDi 8919198.
    KEGGi hvo:HVO_B0205.

    Phylogenomic databases

    HOGENOMi HOG000096435.
    OMAi TYYEVTM.

    Enzyme and pathway databases

    BioCyci HVOL309800:GCOK-3862-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.420. 3 hits.
    InterProi IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 3 hits.
    PROSITEi PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
    2. "LccA, an archaeal laccase secreted as a highly stable glycoprotein into the extracellular medium by Haloferax volcanii."
      Uthandi S., Saad B., Humbard M.A., Maupin-Furlow J.A.
      Appl. Environ. Microbiol. 76:733-743(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, GLYCOSYLATION, ENZYME REGULATION.
      Strain: DS2 / DS70.

    Entry informationi

    Entry nameiLACC_HALVD
    AccessioniPrimary (citable) accession number: D4GPK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3