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D4GPK6

- LACC_HALVD

UniProt

D4GPK6 - LACC_HALVD

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Protein

Laccase

Gene

lccA

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of a wide variety of organic substrates, including bilirubin, syringaldazine (SGZ), 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) and dimethoxyphenol (DMP). No oxidation of Fe2+ or guaiacol.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Cu2+CuratedNote: Binds 4 copper ions per subunit.Curated

Enzyme regulationi

Inhibited by 1 mM NaN3, 10 mM thiourea, 10 mM 1,10-phenanthroline, 0.1 mM DL-dithiothreitol (DTT) and 1 mM L-cysteine. The inhibition by DTT and L-cysteine is likely caused by reduction of the oxidized substrate and not by inhibition of the enzyme.1 Publication

Absorptioni

Abs(max)=605 nm1 Publication

Kineticsi

Kcat is 9.9 sec(-1) with 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid as substrate. Kcat is 21.7 sec(-1) with syringaldazine as substrate. Optimal activity at 200 mM salt, with 65% activity at 1 M NaCl.

  1. KM=671 µM for 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid1 Publication
  2. KM=35 µM for syringaldazine1 Publication

pH dependencei

Optimum pH is 6.0 for the oxidation of 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid and 8.4 for the oxidation of syringaldazine.1 Publication

Temperature dependencei

Optimum temperature is 45-50 degrees Celsius. Highly thermostable.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi145 – 1451Copper 1; type 2By similarity
Metal bindingi147 – 1471Copper 2; type 3By similarity
Metal bindingi192 – 1921Copper 2; type 3By similarity
Metal bindingi194 – 1941Copper 3; type 3By similarity
Metal bindingi455 – 4551Copper 4; type 1By similarity
Metal bindingi458 – 4581Copper 1; type 2By similarity
Metal bindingi460 – 4601Copper 3; type 3By similarity
Metal bindingi512 – 5121Copper 3; type 3By similarity
Metal bindingi513 – 5131Copper 4; type 1By similarity
Metal bindingi514 – 5141Copper 2; type 3By similarity
Metal bindingi518 – 5181Copper 4; type 1By similarity
Metal bindingi523 – 5231Copper 4; type 1By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciHVOL309800:GCOK-3862-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase (EC:1.10.3.2)
Alternative name(s):
LccA multicopper oxidase
Gene namesi
Name:lccA
Ordered Locus Names:HVO_B0205
Encoded oniPlasmid pHV30 Publication
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
ProteomesiUP000008243: Plasmid pHV3

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
BLAST
Chaini32 – 579548LaccasePRO_0000428861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Exported by the Tat system.
Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 214133Plastocyanin-like 1Add
BLAST
Domaini423 – 530108Plastocyanin-like 2Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 2 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000096435.
OMAiTYYEVTM.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D4GPK6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDWSRRRFL QTGAALGIAG TLPQTTTEVS AASPTLEKFV QPLPIPSVRE
60 70 80 90 100
PDGQRDGADA YEIAVTEFTQ QLHPDLPETT VWGFDGSYPG PTIEADAGSP
110 120 130 140 150
VHVRFDNSGL PSEHLFPVDD RLGGTTAENH PGYDGPVPEV RTVTHFHGLE
160 170 180 190 200
LDPANDGQSD MWTSPGGVEG PRFDSAWQEL PMEQGRTTST YHDHTLGITR
210 220 230 240 250
LNAYAGLLGL YSITTDAERE LGLPSGDYDI PLLLQDKEFN DDGSLHYPEE
260 270 280 290 300
FVSAFLGDTA VVNGAVWPYV EVEPRRYRFR ILNGANHRSF DLQLESESGS
310 320 330 340 350
GVPTMYQFAP GHGFLESVVP IGPNGDLDSL LLTPFERGEL VVDFSDHAGE
360 370 380 390 400
TLTLANGADM GPELTDLVEF RVSDPSTPPE DASADPTSLS LPTPASYDES
410 420 430 440 450
DARVTREMTL GTEVRNGLIT HTLNGHVFGD EDAPVYPQLG ATEIWELQNE
460 470 480 490 500
SGGRHPIHLH LVTFRVIGRG PDGTQPPDPN ELGPKDTVRV DPGERVRILV
510 520 530 540 550
TFEGYTGQFP WHCHMLEHED NKMMIPFVVE NPVADYANEE NVVDATGLTD
560 570
AVGDWRNETL ETEVLLEVID QWRSGDEVA
Length:579
Mass (Da):63,442
Last modified:May 18, 2010 - v1
Checksum:i567DF329448F0C06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001953 Genomic DNA. Translation: ADE01515.1.
RefSeqiWP_013034979.1. NC_013964.1.
YP_003533285.1. NC_013964.1.

Genome annotation databases

EnsemblBacteriaiADE01515; ADE01515; HVO_B0205.
GeneIDi8919198.
KEGGihvo:HVO_B0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001953 Genomic DNA. Translation: ADE01515.1 .
RefSeqi WP_013034979.1. NC_013964.1.
YP_003533285.1. NC_013964.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADE01515 ; ADE01515 ; HVO_B0205 .
GeneIDi 8919198.
KEGGi hvo:HVO_B0205.

Phylogenomic databases

HOGENOMi HOG000096435.
OMAi TYYEVTM.

Enzyme and pathway databases

BioCyci HVOL309800:GCOK-3862-MONOMER.

Family and domain databases

Gene3Di 2.60.40.420. 3 hits.
InterProi IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view ]
SUPFAMi SSF49503. SSF49503. 3 hits.
PROSITEi PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  2. "LccA, an archaeal laccase secreted as a highly stable glycoprotein into the extracellular medium by Haloferax volcanii."
    Uthandi S., Saad B., Humbard M.A., Maupin-Furlow J.A.
    Appl. Environ. Microbiol. 76:733-743(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, GLYCOSYLATION, ENZYME REGULATION.
    Strain: DS2 / DS70.

Entry informationi

Entry nameiLACC_HALVD
AccessioniPrimary (citable) accession number: D4GPK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 14, 2014
Last sequence update: May 18, 2010
Last modified: November 26, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3