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Protein

Laccase

Gene

lccA

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of a wide variety of organic substrates, including bilirubin, syringaldazine (SGZ), 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) and dimethoxyphenol (DMP). No oxidation of Fe2+ or guaiacol.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Cu2+CuratedNote: Binds 4 copper ions per subunit.Curated

Enzyme regulationi

Inhibited by 1 mM NaN3, 10 mM thiourea, 10 mM 1,10-phenanthroline, 0.1 mM DL-dithiothreitol (DTT) and 1 mM L-cysteine. The inhibition by DTT and L-cysteine is likely caused by reduction of the oxidized substrate and not by inhibition of the enzyme.1 Publication

Absorptioni

Abs(max)=605 nm1 Publication

Kineticsi

Kcat is 9.9 sec(-1) with 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid as substrate. Kcat is 21.7 sec(-1) with syringaldazine as substrate. Optimal activity at 200 mM salt, with 65% activity at 1 M NaCl.

  1. KM=671 µM for 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid1 Publication
  2. KM=35 µM for syringaldazine1 Publication

    pH dependencei

    Optimum pH is 6.0 for the oxidation of 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid and 8.4 for the oxidation of syringaldazine.1 Publication

    Temperature dependencei

    Optimum temperature is 45-50 degrees Celsius. Highly thermostable.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi145 – 1451Copper 1; type 2By similarity
    Metal bindingi147 – 1471Copper 2; type 3By similarity
    Metal bindingi192 – 1921Copper 2; type 3By similarity
    Metal bindingi194 – 1941Copper 3; type 3By similarity
    Metal bindingi455 – 4551Copper 4; type 1By similarity
    Metal bindingi458 – 4581Copper 1; type 2By similarity
    Metal bindingi460 – 4601Copper 3; type 3By similarity
    Metal bindingi512 – 5121Copper 3; type 3By similarity
    Metal bindingi513 – 5131Copper 4; type 1By similarity
    Metal bindingi514 – 5141Copper 2; type 3By similarity
    Metal bindingi518 – 5181Copper 4; type 1By similarity
    Metal bindingi523 – 5231Copper 4; type 1By similarity

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    BioCyciHVOL309800:GCOK-3862-MONOMER.
    BRENDAi1.10.3.2. 2561.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase (EC:1.10.3.2)
    Alternative name(s):
    LccA multicopper oxidase
    Gene namesi
    Name:lccA
    Ordered Locus Names:HVO_B0205
    Encoded oniPlasmid pHV30 Publication
    OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
    Taxonomic identifieri309800 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloferax
    ProteomesiUP000008243 Componenti: Plasmid pHV3

    Subcellular locationi

    • Secreted 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalPROSITE-ProRule annotation1 PublicationAdd
    BLAST
    Chaini32 – 579548LaccasePRO_0000428861Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Exported by the Tat system.
    Glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini82 – 214133Plastocyanin-like 1Add
    BLAST
    Domaini423 – 530108Plastocyanin-like 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 2 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOGENOMiHOG000096435.
    OMAiKLKMFVD.

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    D4GPK6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTDWSRRRFL QTGAALGIAG TLPQTTTEVS AASPTLEKFV QPLPIPSVRE
    60 70 80 90 100
    PDGQRDGADA YEIAVTEFTQ QLHPDLPETT VWGFDGSYPG PTIEADAGSP
    110 120 130 140 150
    VHVRFDNSGL PSEHLFPVDD RLGGTTAENH PGYDGPVPEV RTVTHFHGLE
    160 170 180 190 200
    LDPANDGQSD MWTSPGGVEG PRFDSAWQEL PMEQGRTTST YHDHTLGITR
    210 220 230 240 250
    LNAYAGLLGL YSITTDAERE LGLPSGDYDI PLLLQDKEFN DDGSLHYPEE
    260 270 280 290 300
    FVSAFLGDTA VVNGAVWPYV EVEPRRYRFR ILNGANHRSF DLQLESESGS
    310 320 330 340 350
    GVPTMYQFAP GHGFLESVVP IGPNGDLDSL LLTPFERGEL VVDFSDHAGE
    360 370 380 390 400
    TLTLANGADM GPELTDLVEF RVSDPSTPPE DASADPTSLS LPTPASYDES
    410 420 430 440 450
    DARVTREMTL GTEVRNGLIT HTLNGHVFGD EDAPVYPQLG ATEIWELQNE
    460 470 480 490 500
    SGGRHPIHLH LVTFRVIGRG PDGTQPPDPN ELGPKDTVRV DPGERVRILV
    510 520 530 540 550
    TFEGYTGQFP WHCHMLEHED NKMMIPFVVE NPVADYANEE NVVDATGLTD
    560 570
    AVGDWRNETL ETEVLLEVID QWRSGDEVA
    Length:579
    Mass (Da):63,442
    Last modified:May 18, 2010 - v1
    Checksum:i567DF329448F0C06
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP001953 Genomic DNA. Translation: ADE01515.1.
    RefSeqiWP_013034979.1. NC_013964.1.
    YP_003533285.1. NC_013964.1.

    Genome annotation databases

    EnsemblBacteriaiADE01515; ADE01515; HVO_B0205.
    GeneIDi8919198.
    KEGGihvo:HVO_B0205.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP001953 Genomic DNA. Translation: ADE01515.1.
    RefSeqiWP_013034979.1. NC_013964.1.
    YP_003533285.1. NC_013964.1.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiADE01515; ADE01515; HVO_B0205.
    GeneIDi8919198.
    KEGGihvo:HVO_B0205.

    Phylogenomic databases

    HOGENOMiHOG000096435.
    OMAiKLKMFVD.

    Enzyme and pathway databases

    BioCyciHVOL309800:GCOK-3862-MONOMER.
    BRENDAi1.10.3.2. 2561.

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
    2. "LccA, an archaeal laccase secreted as a highly stable glycoprotein into the extracellular medium by Haloferax volcanii."
      Uthandi S., Saad B., Humbard M.A., Maupin-Furlow J.A.
      Appl. Environ. Microbiol. 76:733-743(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, GLYCOSYLATION, ENZYME REGULATION.
      Strain: DS2 / DS70.

    Entry informationi

    Entry nameiLACC_HALVD
    AccessioniPrimary (citable) accession number: D4GPK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: May 18, 2010
    Last modified: July 22, 2015
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.