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Protein

Laccase

Gene

lccA

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of a wide variety of organic substrates, including bilirubin, syringaldazine (SGZ), 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) and dimethoxyphenol (DMP). No oxidation of Fe2+ or guaiacol.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Cu2+CuratedNote: Binds 4 copper ions per subunit.Curated

Enzyme regulationi

Inhibited by 1 mM NaN3, 10 mM thiourea, 10 mM 1,10-phenanthroline, 0.1 mM DL-dithiothreitol (DTT) and 1 mM L-cysteine. The inhibition by DTT and L-cysteine is likely caused by reduction of the oxidized substrate and not by inhibition of the enzyme.1 Publication

Absorptioni

Abs(max)=605 nm1 Publication

Kineticsi

Kcat is 9.9 sec(-1) with 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid as substrate. Kcat is 21.7 sec(-1) with syringaldazine as substrate. Optimal activity at 200 mM salt, with 65% activity at 1 M NaCl.

  1. KM=671 µM for 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid1 Publication
  2. KM=35 µM for syringaldazine1 Publication

    pH dependencei

    Optimum pH is 6.0 for the oxidation of 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid and 8.4 for the oxidation of syringaldazine.1 Publication

    Temperature dependencei

    Optimum temperature is 45-50 degrees Celsius. Highly thermostable.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi145Copper 1; type 2By similarity1
    Metal bindingi147Copper 2; type 3By similarity1
    Metal bindingi192Copper 2; type 3By similarity1
    Metal bindingi194Copper 3; type 3By similarity1
    Metal bindingi455Copper 4; type 1By similarity1
    Metal bindingi458Copper 1; type 2By similarity1
    Metal bindingi460Copper 3; type 3By similarity1
    Metal bindingi512Copper 3; type 3By similarity1
    Metal bindingi513Copper 4; type 1By similarity1
    Metal bindingi514Copper 2; type 3By similarity1
    Metal bindingi518Copper 4; type 1By similarity1
    Metal bindingi523Copper 4; type 1By similarity1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    BRENDAi1.10.3.2. 2561.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase (EC:1.10.3.2)
    Alternative name(s):
    LccA multicopper oxidase
    Gene namesi
    Name:lccA
    Ordered Locus Names:HVO_B0205
    Encoded oniPlasmid pHV30 Publication
    OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
    Taxonomic identifieri309800 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloferax
    Proteomesi
    • UP000008243 Componenti: Plasmid pHV3

    Subcellular locationi

    • Secreted 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 31Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST31
    ChainiPRO_000042886132 – 579LaccaseAdd BLAST548

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi449N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi557N-linked (GlcNAc...)Sequence analysis1

    Post-translational modificationi

    Exported by the Tat system.
    Glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    SMRiD4GPK6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini82 – 214Plastocyanin-like 1Add BLAST133
    Domaini423 – 530Plastocyanin-like 2Add BLAST108

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 2 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOGENOMiHOG000096435.
    OMAiPYSGRYV.

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    D4GPK6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTDWSRRRFL QTGAALGIAG TLPQTTTEVS AASPTLEKFV QPLPIPSVRE
    60 70 80 90 100
    PDGQRDGADA YEIAVTEFTQ QLHPDLPETT VWGFDGSYPG PTIEADAGSP
    110 120 130 140 150
    VHVRFDNSGL PSEHLFPVDD RLGGTTAENH PGYDGPVPEV RTVTHFHGLE
    160 170 180 190 200
    LDPANDGQSD MWTSPGGVEG PRFDSAWQEL PMEQGRTTST YHDHTLGITR
    210 220 230 240 250
    LNAYAGLLGL YSITTDAERE LGLPSGDYDI PLLLQDKEFN DDGSLHYPEE
    260 270 280 290 300
    FVSAFLGDTA VVNGAVWPYV EVEPRRYRFR ILNGANHRSF DLQLESESGS
    310 320 330 340 350
    GVPTMYQFAP GHGFLESVVP IGPNGDLDSL LLTPFERGEL VVDFSDHAGE
    360 370 380 390 400
    TLTLANGADM GPELTDLVEF RVSDPSTPPE DASADPTSLS LPTPASYDES
    410 420 430 440 450
    DARVTREMTL GTEVRNGLIT HTLNGHVFGD EDAPVYPQLG ATEIWELQNE
    460 470 480 490 500
    SGGRHPIHLH LVTFRVIGRG PDGTQPPDPN ELGPKDTVRV DPGERVRILV
    510 520 530 540 550
    TFEGYTGQFP WHCHMLEHED NKMMIPFVVE NPVADYANEE NVVDATGLTD
    560 570
    AVGDWRNETL ETEVLLEVID QWRSGDEVA
    Length:579
    Mass (Da):63,442
    Last modified:May 18, 2010 - v1
    Checksum:i567DF329448F0C06
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP001953 Genomic DNA. Translation: ADE01515.1.

    Genome annotation databases

    EnsemblBacteriaiADE01515; ADE01515; HVO_B0205.
    KEGGihvo:HVO_B0205.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP001953 Genomic DNA. Translation: ADE01515.1.

    3D structure databases

    SMRiD4GPK6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiADE01515; ADE01515; HVO_B0205.
    KEGGihvo:HVO_B0205.

    Phylogenomic databases

    HOGENOMiHOG000096435.
    OMAiPYSGRYV.

    Enzyme and pathway databases

    BRENDAi1.10.3.2. 2561.

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR008972. Cupredoxin.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS51318. TAT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiLACC_HALVD
    AccessioniPrimary (citable) accession number: D4GPK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 14, 2014
    Last sequence update: May 18, 2010
    Last modified: November 30, 2016
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.