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D4GPK6

- LACC_HALVD

UniProt

D4GPK6 - LACC_HALVD

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Protein

Laccase

Gene
lccA, HVO_B0205
Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of a wide variety of organic substrates, including bilirubin, syringaldazine (SGZ), 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) and dimethoxyphenol (DMP). No oxidation of Fe2+ or guaiacol.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Binds 4 copper ions per subunit Inferred.

Enzyme regulationi

Inhibited by 1 mM NaN3, 10 mM thiourea, 10 mM 1,10-phenanthroline, 0.1 mM DL-dithiothreitol (DTT) and 1 mM L-cysteine. The inhibition by DTT and L-cysteine is likely caused by reduction of the oxidized substrate and not by inhibition of the enzyme.1 Publication

Absorptioni

Abs(max)=605 nm1 Publication

Kineticsi

Kcat is 9.9 sec(-1) with 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid as substrate. Kcat is 21.7 sec(-1) with syringaldazine as substrate. Optimal activity at 200 mM salt, with 65% activity at 1 M NaCl.

  1. KM=671 µM for 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  2. KM=35 µM for syringaldazine

pH dependencei

Optimum pH is 6.0 for the oxidation of 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid and 8.4 for the oxidation of syringaldazine.

Temperature dependencei

Optimum temperature is 45-50 degrees Celsius. Highly thermostable.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi145 – 1451Copper 1; type 2 By similarity
Metal bindingi147 – 1471Copper 2; type 3 By similarity
Metal bindingi192 – 1921Copper 2; type 3 By similarity
Metal bindingi194 – 1941Copper 3; type 3 By similarity
Metal bindingi455 – 4551Copper 4; type 1 By similarity
Metal bindingi458 – 4581Copper 1; type 2 By similarity
Metal bindingi460 – 4601Copper 3; type 3 By similarity
Metal bindingi512 – 5121Copper 3; type 3 By similarity
Metal bindingi513 – 5131Copper 4; type 1 By similarity
Metal bindingi514 – 5141Copper 2; type 3 By similarity
Metal bindingi518 – 5181Copper 4; type 1 By similarity
Metal bindingi523 – 5231Copper 4; type 1 By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciHVOL309800:GCOK-3862-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase (EC:1.10.3.2)
Alternative name(s):
LccA multicopper oxidase
Gene namesi
Name:lccA
Ordered Locus Names:HVO_B0205
Encoded oniPlasmid pHV30 Publication
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
ProteomesiUP000008243: Plasmid pHV3

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signal1 PublicationAdd
BLAST
Chaini32 – 579548LaccasePRO_0000428861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi449 – 4491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi557 – 5571N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Exported by the Tat system.
Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 214133Plastocyanin-like 1Add
BLAST
Domaini423 – 530108Plastocyanin-like 2Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000096435.
OMAiTYYEVTM.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D4GPK6-1 [UniParc]FASTAAdd to Basket

« Hide

MTDWSRRRFL QTGAALGIAG TLPQTTTEVS AASPTLEKFV QPLPIPSVRE    50
PDGQRDGADA YEIAVTEFTQ QLHPDLPETT VWGFDGSYPG PTIEADAGSP 100
VHVRFDNSGL PSEHLFPVDD RLGGTTAENH PGYDGPVPEV RTVTHFHGLE 150
LDPANDGQSD MWTSPGGVEG PRFDSAWQEL PMEQGRTTST YHDHTLGITR 200
LNAYAGLLGL YSITTDAERE LGLPSGDYDI PLLLQDKEFN DDGSLHYPEE 250
FVSAFLGDTA VVNGAVWPYV EVEPRRYRFR ILNGANHRSF DLQLESESGS 300
GVPTMYQFAP GHGFLESVVP IGPNGDLDSL LLTPFERGEL VVDFSDHAGE 350
TLTLANGADM GPELTDLVEF RVSDPSTPPE DASADPTSLS LPTPASYDES 400
DARVTREMTL GTEVRNGLIT HTLNGHVFGD EDAPVYPQLG ATEIWELQNE 450
SGGRHPIHLH LVTFRVIGRG PDGTQPPDPN ELGPKDTVRV DPGERVRILV 500
TFEGYTGQFP WHCHMLEHED NKMMIPFVVE NPVADYANEE NVVDATGLTD 550
AVGDWRNETL ETEVLLEVID QWRSGDEVA 579
Length:579
Mass (Da):63,442
Last modified:May 18, 2010 - v1
Checksum:i567DF329448F0C06
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001953 Genomic DNA. Translation: ADE01515.1.
RefSeqiYP_003533285.1. NC_013964.1.

Genome annotation databases

EnsemblBacteriaiADE01515; ADE01515; HVO_B0205.
GeneIDi8919198.
KEGGihvo:HVO_B0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001953 Genomic DNA. Translation: ADE01515.1 .
RefSeqi YP_003533285.1. NC_013964.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADE01515 ; ADE01515 ; HVO_B0205 .
GeneIDi 8919198.
KEGGi hvo:HVO_B0205.

Phylogenomic databases

HOGENOMi HOG000096435.
OMAi TYYEVTM.

Enzyme and pathway databases

BioCyci HVOL309800:GCOK-3862-MONOMER.

Family and domain databases

Gene3Di 2.60.40.420. 3 hits.
InterProi IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view ]
SUPFAMi SSF49503. SSF49503. 3 hits.
PROSITEi PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  2. "LccA, an archaeal laccase secreted as a highly stable glycoprotein into the extracellular medium by Haloferax volcanii."
    Uthandi S., Saad B., Humbard M.A., Maupin-Furlow J.A.
    Appl. Environ. Microbiol. 76:733-743(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, GLYCOSYLATION, ENZYME REGULATION.
    Strain: DS2 / DS70.

Entry informationi

Entry nameiLACC_HALVD
AccessioniPrimary (citable) accession number: D4GPK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 14, 2014
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi