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D4GJS8 (D4GJS8_PANAM) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlB EMBL ADD75888.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:PANA_0721 EMBL ADD75888.1
OrganismPantoea ananatis (strain LMG 20103) [Complete proteome] [HAMAP] EMBL ADD75888.1
Taxonomic identifier706191 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain100 – 302203ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding133 – 18856ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2561Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2691Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2691Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2711Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D4GJS8 [UniParc].

Last modified May 18, 2010. Version 1.
Checksum: 6B91C3CCD5DE3080

FASTA30532,653
        10         20         30         40         50         60 
MTKVAVLMGG TSAERDVSLM SGGAVLAGLR EAGIDAQGID TRDVSVLELT AQGFEKVFIA 

        70         80         90        100        110        120 
LHGRGGEDGT LQAVLEFLQL PYTGSGVMAS AVTMDKLRTK LLWQGRGLPA GKFVWITREQ 

       130        140        150        160        170        180 
MQSGLDAATE AAISALGLPL FVKPSSEGSS VGISRVNQLS DLSAALEEAF RHDDNVLVEA 

       190        200        210        220        230        240 
FLSGEEYTVG ILGDQILPAI KIKTASEFYD YEAKYISDDT QYFCPAGLSA EREAELQQLV 

       250        260        270        280        290        300 
WSAWRALGCS GWGRVDVMTD DQGHFQLLEA NTSPGMTSHS LVPMAAKQAG LTFSQLVVRI 


LELAD 

« Hide

References

[1]"Genome sequence of Pantoea ananatis LMG20103, the causative agent of Eucalyptus blight and dieback."
De Maayer P., Chan W.Y., Venter S.N., Toth I.K., Birch P.R., Joubert F., Coutinho T.A.
J. Bacteriol. 192:2936-2937(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LMG 20103 EMBL ADD75888.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001875 Genomic DNA. Translation: ADD75888.1.
RefSeqYP_003519016.1. NC_013956.2.

3D structure databases

ProteinModelPortalD4GJS8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADD75888; ADD75888; PANA_0721.
GeneID8894558.
KEGGpam:PANA_0721.
PATRIC35382255. VBIPanAna151616_0748.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.

Enzyme and pathway databases

BioCycPANA706191:GJNK-749-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD4GJS8_PANAM
AccessionPrimary (citable) accession number: D4GJS8
Entry history
Integrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: February 19, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)