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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Bacillus subtilis subsp. natto (strain BEST195)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17NAD; via amide nitrogenUniRule annotation1
Binding sitei38NADUniRule annotation1
Binding sitei43NADUniRule annotation1
Binding sitei69NADUniRule annotation1
Binding sitei86SubstrateUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Binding sitei124SubstrateUniRule annotation1
Binding sitei147NADUniRule annotation1
Binding sitei155SubstrateUniRule annotation1
Binding sitei157Allosteric activatorUniRule annotation1
Binding sitei172Allosteric activatorUniRule annotation1
Active sitei179Proton acceptorUniRule annotation1
Binding sitei233SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi83 – 84NADUniRule annotation2
Nucleotide bindingi122 – 124NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzymeUniRule annotation, OxidoreductaseUniRule annotation
LigandNADUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotationImported
ORF Names:BSNT_06628Imported
OrganismiBacillus subtilis subsp. natto (strain BEST195)Imported
Taxonomic identifieri645657 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000006805 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineUniRule annotation1

Keywords - PTMi

PhosphoproteinUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD4G4W2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 146Ldh_1_NInterPro annotationAdd BLAST139
Domaini149 – 317Ldh_1_CInterPro annotationAdd BLAST169

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 127Substrate bindingUniRule annotation4
Regioni152 – 155Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

KOiK00016
OrthoDBiPOG091H02EQ

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

D4G4W2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNKHVNKVA LIGAGFVGSS YAFALINQGI TDELVVIDVN KEKAMGDVMD
60 70 80 90 100
LNHGKAFAPQ PVKTSYGTYE DCKDADIVCI CAGANQKPGE TRLELVEKNL
110 120 130 140 150
KIFKGIVSEV MASGFDGIFL IATNPVDILT YATWKFSGLP KERVIGSGTT
160 170 180 190 200
LDSARFRFML SEYFGAAPQN VHAHIIGEHG DTELPVWSHA NVGGVPVSEL
210 220 230 240 250
VEKNDAYKQE ELDQIVDDVK NAAYHIIEKK GATYYGVAMS LARITKAILH
260 270 280 290 300
NENSILTVST YLDGQYGADD VYIGVPAVVN RGGIAGITEL NLNEKEKEQF
310 320
LHSAGVLKNI LKPHFAEQKV N
Length:321
Mass (Da):34,947
Last modified:May 18, 2010 - v1
Checksum:i935123ABA48940C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011541 Genomic DNA Translation: BAI83768.1
RefSeqiWP_014478761.1, NC_017196.2

Genome annotation databases

EnsemblBacteriaiBAI83768; BAI83768; BSNT_06628
KEGGibso:BSNT_06628
PATRICifig|645657.3.peg.1192

Similar proteinsi

Entry informationi

Entry nameiD4G4W2_BACNB
AccessioniPrimary (citable) accession number: D4G4W2
Entry historyiIntegrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: June 20, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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