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Protein

Alpha-amylase

Gene

BSNT_06626

Organism
Bacillus subtilis subsp. natto (strain BEST195)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
ORF Names:BSNT_06626Imported
OrganismiBacillus subtilis subsp. natto (strain BEST195)Imported
Taxonomic identifieri645657 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000006805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence analysisAdd
BLAST
Chaini34 – 477444Alpha-amylaseSequence analysisPRO_5003057520Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliD4G4W0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 383334AamyInterPro annotationAdd
BLAST
Domaini393 – 46876Aamy_CInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

KOiK01176.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

D4G4W0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFAKRFKTSL LPLFAGFLLL FYLVLAGPAA ASAETANKSN ELTAPSIKSG
60 70 80 90 100
TILHAWNWSF NTLKHNMKDI HDAGYTAIQT SPINQVKEGN QGDKSMSNWY
110 120 130 140 150
WLYQPTSYQI GNRYLGTEQE FKEMCAAAEE YGIKVIVDAV INHTTSDYAA
160 170 180 190 200
ISNEVKSIPN WTHGNTQIKN WSDRWDVTQN SLPGLYDWNT QNTQVQSYLK
210 220 230 240 250
RFLERALNDG ADGFRFDAAK HIELPDDGSY GSQFWPNITN TSAEFQYGEI
260 270 280 290 300
LQDSASRDAA YANYMDVTAS NYGHSIRSAL KNRNLGVSNI SHYASDVSAD
310 320 330 340 350
KLVTWVESHD TYANDDEEST WMSDDDIRLG WAVIASRSGS TPLFFSRPEG
360 370 380 390 400
GGNGVRFPGK SQIGDRGSAL FEDQAITAVN RFHNVMAGQH EELSNPNGNN
410 420 430 440 450
QIFMNQRGSH GVVLANAGSS SVSINTATKL PDGRYDNKAG AGSFQVNDGK
460 470
LTGTINARSV AVLYPDDIEI RCNTFFQ
Length:477
Mass (Da):52,757
Last modified:May 18, 2010 - v1
Checksum:i9F9F435223C73756
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011541 Genomic DNA. Translation: BAI83766.1.

Genome annotation databases

EnsemblBacteriaiBAI83766; BAI83766; BSNT_06626.
KEGGibso:BSNT_06626.
PATRICi42727943. VBIBacSub97792_1190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011541 Genomic DNA. Translation: BAI83766.1.

3D structure databases

ProteinModelPortaliD4G4W0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAI83766; BAI83766; BSNT_06626.
KEGGibso:BSNT_06626.
PATRICi42727943. VBIBacSub97792_1190.

Phylogenomic databases

KOiK01176.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data."
    Nishito Y., Osana Y., Hachiya T., Popendorf K., Toyoda A., Fujiyama A., Itaya M., Sakakibara Y.
    BMC Genomics 11:243-243(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BEST195Imported.
  2. "Whole genome complete resequencing of Bacillus subtilis natto by combining long reads with high-quality short reads."
    Kamada M., Hase S., Sato K., Toyoda A., Fujiyama A., Sakakibara Y.
    PLoS ONE 9:E109999-E109999(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: BEST195Imported.

Entry informationi

Entry nameiD4G4W0_BACNB
AccessioniPrimary (citable) accession number: D4G4W0
Entry historyi
Integrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: June 8, 2016
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.