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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Bacillus subtilis subsp. natto (strain BEST195)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferaseUniRule annotation, Transferase
Biological processTranscription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotationImported
ORF Names:BSNT_06428Imported
OrganismiBacillus subtilis subsp. natto (strain BEST195)Imported
Taxonomic identifieri645657 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000006805 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymeraseUniRule annotationImported

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliD4G4D4.
SMRiD4G4D4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 227RPOLDInterPro annotationAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 230Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST230
Regioni247 – 314Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST68

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili231 – 251Sequence analysisAdd BLAST21

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

KOiK03040.
OrthoDBiPOG091H001Z.

Family and domain databases

Gene3Di3.30.1360.10. 2 hits.
HAMAPiMF_00059. RNApol_bact_RpoA. 1 hit.
InterProiView protein in InterPro
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR036603. RBP11-like.
IPR011260. RNAP_asu_C.
IPR036643. RNApol_insert_sf.
PfamiView protein in Pfam
PF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179. RNAP_asu_C. 1 hit.
SMARTiView protein in SMART
SM00662. RPOLD. 1 hit.
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.

Sequencei

Sequence statusi: Complete.

D4G4D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIEKPKIE TVEISDDAKF GKFVVEPLER GYGTTLGNSL RRILLSSLPG
60 70 80 90 100
AAVTSIQIDG VLHEFSTIEG VVEDVTTIIL HIKKLALKIY SDEEKTLEID
110 120 130 140 150
VQGEGTVTAA DITHDSDVEI LNPDLHIATL GENASFRVRL TAQRGRGYTP
160 170 180 190 200
ADANKRDDQP IGVIPIDSIY TPVSRVSYQV ENTRVGQVAN YDKLTLDVWT
210 220 230 240 250
DGSTGPKEAI ALGSKILTEH LNIFVGLTDE AQHAEIMVEK EEDQKEKVLE
260 270 280 290 300
MTIEELDLSV RSYNCLKRAG INTVQELANK TEEDMMKVRN LGRKSLEEVK
310
AKLEELGLGL RKDD
Length:314
Mass (Da):34,799
Last modified:May 18, 2010 - v1
Checksum:i9ADCAD891C0BCD67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP011541 Genomic DNA. Translation: BAI83590.1.
RefSeqiWP_003225835.1. NC_017196.2.

Genome annotation databases

EnsemblBacteriaiBAI83590; BAI83590; BSNT_06428.
KEGGibso:BSNT_06428.
PATRICifig|645657.3.peg.150.

Similar proteinsi

Entry informationi

Entry nameiD4G4D4_BACNB
AccessioniPrimary (citable) accession number: D4G4D4
Entry historyiIntegrated into UniProtKB/TrEMBL: May 18, 2010
Last sequence update: May 18, 2010
Last modified: December 20, 2017
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported