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Protein

Inositol monophosphatase 3

Gene

Impad1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation.By similarity

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.

Cofactori

Mg2+By similarity

Enzyme regulationi

Strongly inhibited by lithium.

Pathwayi: myo-inositol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Inositol-3-phosphate synthase 1 (Isyna1)
  2. Inositol monophosphatase 3 (Impad1), Inositol monophosphatase 1 (Impa1), Inositol monophosphatase 2 (Impa2)
This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311Magnesium 1By similarity
Binding sitei131 – 1311SubstrateBy similarity
Metal bindingi172 – 1721Magnesium 1By similarity
Metal bindingi172 – 1721Magnesium 2By similarity
Metal bindingi174 – 1741Magnesium 1; via carbonyl oxygenBy similarity
Metal bindingi175 – 1751Magnesium 2By similarity
Metal bindingi298 – 2981Magnesium 2By similarity
Binding sitei298 – 2981SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol monophosphatase 3 (EC:3.1.3.25, EC:3.1.3.7)
Short name:
IMP 3
Short name:
IMPase 3
Alternative name(s):
Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase
Short name:
Golgi-resident PAP phosphatase
Short name:
gPAPP
Inositol monophosphatase domain-containing protein 1
Inositol-1(or 4)-monophosphatase 3
Myo-inositol monophosphatase A3
Gene namesi
Name:Impad1
Synonyms:Impa3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componentsi: Chromosome 17, Chromosome 5

Organism-specific databases

RGDi1306455. Impad1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321HelicalSequence analysisAdd
BLAST
Topological domaini34 – 356323LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Inositol monophosphatase 3PRO_0000413415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi257 – 2571N-linked (GlcNAc...)Curated

Post-translational modificationi

Contains N-linked glycan resistant to endoglycosydase H.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiD4AD37.
PeptideAtlasiD4AD37.
PRIDEiD4AD37.

PTM databases

iPTMnetiD4AD37.

Expressioni

Gene expression databases

ExpressionAtlasiD4AD37. baseline and differential.
GenevisibleiD4AD37. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066904.

Structurei

3D structure databases

ProteinModelPortaliD4AD37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni174 – 1774Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3853. Eukaryota.
COG1218. LUCA.
GeneTreeiENSGT00530000063462.
InParanoidiD4AD37.
KOiK15759.
PhylomeDBiD4AD37.
TreeFamiTF314300.

Family and domain databases

InterProiIPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PROSITEiPS00630. IMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D4AD37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPMGIRLSP LGVAVFFLLG LGVLYHLYSG FLAGRFSLFG LGGEPAGGAA
60 70 80 90 100
EVAVDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA
110 120 130 140 150
EDKMTSGDVL SNRKMFYLLK TAFPNVQINT EEHVDASDKE VIVWNRKIPE
160 170 180 190 200
DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP
210 220 230 240 250
VLGVIHKPFS EYTAWAMVDS GSNVKARSSY NEKTPKIIVS RSHAGMVKQV
260 270 280 290 300
ALQTFGNQTL IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC
310 320 330 340 350
AGNAILKALG GHMTTLSGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD

LEKSGH
Length:356
Mass (Da):38,569
Last modified:April 20, 2010 - v1
Checksum:i847D257B4C40DBE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473984 Genomic DNA. Translation: EDM11627.1.
RefSeqiNP_001008772.2. NM_001008772.2.
XP_008761786.1. XM_008763564.1.
XP_008774114.1. XM_008775892.1.
UniGeneiRn.65720.

Genome annotation databases

EnsembliENSRNOT00000034495; ENSRNOP00000038358; ENSRNOG00000027079.
ENSRNOT00000072803; ENSRNOP00000067120; ENSRNOG00000046647.
GeneIDi103689950.
312952.
KEGGirno:103689950.
rno:312952.
UCSCiRGD:1306455. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473984 Genomic DNA. Translation: EDM11627.1.
RefSeqiNP_001008772.2. NM_001008772.2.
XP_008761786.1. XM_008763564.1.
XP_008774114.1. XM_008775892.1.
UniGeneiRn.65720.

3D structure databases

ProteinModelPortaliD4AD37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066904.

PTM databases

iPTMnetiD4AD37.

Proteomic databases

PaxDbiD4AD37.
PeptideAtlasiD4AD37.
PRIDEiD4AD37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000034495; ENSRNOP00000038358; ENSRNOG00000027079.
ENSRNOT00000072803; ENSRNOP00000067120; ENSRNOG00000046647.
GeneIDi103689950.
312952.
KEGGirno:103689950.
rno:312952.
UCSCiRGD:1306455. rat.

Organism-specific databases

CTDi103689950.
54928.
RGDi1306455. Impad1.

Phylogenomic databases

eggNOGiKOG3853. Eukaryota.
COG1218. LUCA.
GeneTreeiENSGT00530000063462.
InParanoidiD4AD37.
KOiK15759.
PhylomeDBiD4AD37.
TreeFamiTF314300.

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.

Miscellaneous databases

PROiD4AD37.

Gene expression databases

ExpressionAtlasiD4AD37. baseline and differential.
GenevisibleiD4AD37. RN.

Family and domain databases

InterProiIPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PROSITEiPS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiIMPA3_RAT
AccessioniPrimary (citable) accession number: D4AD37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: April 20, 2010
Last modified: July 6, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.