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Protein

Zinc finger and BTB domain-containing protein 4

Gene

Zbtb4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri210 – 23223C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri285 – 30723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri313 – 33523C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri341 – 36424C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri697 – 71923C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri736 – 75823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 4
Gene namesi
Name:Zbtb4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1307184. Zbtb4.

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity

  • Note: Localizes to chromocenters.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 984984Zinc finger and BTB domain-containing protein 4PRO_0000434409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki40 – 40Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei367 – 3671PhosphoserineBy similarity
Cross-linki590 – 590Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei766 – 7661Phosphothreonine; by HIPK2By similarity
Modified residuei768 – 7681Phosphothreonine; by HIPK2By similarity
Modified residuei955 – 9551Phosphothreonine; by HIPK2By similarity

Post-translational modificationi

Phosphorylated by HIPK2. This phosphorylation reduces stability and triggers ZBTB4 protein degradation in response to DNA damage.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiD4A8X0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000014689.

Interactioni

Subunit structurei

Interacts with HIPK2. Interacts with CBFA2T3. Interacts with ZBTB38.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019687.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 131102BTBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni165 – 324160Interaction with CBFA2T3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi585 – 62844Glu-richPROSITE-ProRule annotationAdd
BLAST
Compositional biasi807 – 8148Poly-AlaSequence analysis
Compositional biasi946 – 96621Pro-richPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri210 – 23223C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri285 – 30723C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri313 – 33523C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri341 – 36424C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri697 – 71923C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri736 – 75823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00810000125416.
InParanoidiD4A8X0.
KOiK10491.
OMAiNMVLPDE.
OrthoDBiEOG091G01R6.
PhylomeDBiD4A8X0.
TreeFamiTF333100.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 2 hits.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D4A8X0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPPAEVTDP SHAPAVLRQL NEQRLRGLFC DVTLIAGDTK FPAHRSVLAA
60 70 80 90 100
SSPFFREALL ASAPLPLPPV TGGSAPSPAT TTAASSSSSP PPPASPHSSS
110 120 130 140 150
PPRVLELPGV PAAAFSDVLN FIYSARLALP GGGGDGAAVA EIGALGRRLG
160 170 180 190 200
ISRLQGLGEG GDTWVPPAPS SMVTSEPNED SLGPGLRTDG GWEGDKAEPL
210 220 230 240 250
TPDSQPRRPF PCPRCGKSFI HPKRLQTHEA QCRRGSNTRG SAGLGPGGSG
260 270 280 290 300
PGGPAGVDAS ALPQPVSFRD GPEHVVKVVG GHVLYVCAAC ERSYVTLSSL
310 320 330 340 350
KRHSNVHSWR RKYPCRYCEK VFALAEYRTK HEVWHTGERR YQCIFCWETF
360 370 380 390 400
VTYYNLKTHQ RAFHGISPGL LASEKTPNGG YKPKLNTLKL YRLLPMRAAK
410 420 430 440 450
RPYKTYSQGA PEAPLSPSLH TPVPAVMPAS PQPLLPSVPE PGPPHSVITF
460 470 480 490 500
AHPAPSVIVH GSSSSGAAGG GPVGTGGSQA ASVITYTTPP RPPKKREYPP
510 520 530 540 550
PPPEPAATPT SPASTAVIPA TAAGPATATE EAKGRNLRAG RTLTYTAKPV
560 570 580 590 600
GGVSGSGGSP TGTGRGSSQL QAPPPLCQIT VRIGEEAIVK RRISETDLRP
610 620 630 640 650
GELSGEEVEE SEEEEEEEEE EDQEDQEESK AGGEDQLWRP YYSYKPKRKA
660 670 680 690 700
GATASGLSGL PRGRRPPRWR QKLERRGWEE TPAVEGPGGR GRGERRHRCG
710 720 730 740 750
DCAQAFATLR KLRKHQEAHS GGSHNSRTGR RSSTRFTCPH CAKVCKTAAA
760 770 780 790 800
LNRHGQRHAV ERPGGTPTPV IAYSKGSIGT RPTDVKEEAP QEMQVSSSSG
810 820 830 840 850
EAGGGSAAAA AAEASESASL QDPVISGGEE PPVAGGGGYV YPPVQEFPLA
860 870 880 890 900
LIGGSRDPGA GKGKPGNEGP VGASEGNRME EMGTAKVTFY PEPYPLVYGP
910 920 930 940 950
QLLAAYPYNF SNLAALPVAL NMVLPDEKGG GALPFLPGVF GYAVNPQTAP
960 970 980
PTPPTPPPPL PLPVPPKGVG EMTGVERTQK GDVG
Length:984
Mass (Da):103,210
Last modified:April 20, 2010 - v1
Checksum:iD7223FA77F7A896B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR07029862 Genomic DNA. No translation available.
RefSeqiNP_001291281.1. NM_001304352.1.
XP_006220767.1. XM_006220705.1.
XP_006220768.1. XM_006220706.2.
XP_006246906.1. XM_006246844.2.
UniGeneiRn.15836.
Rn.232400.

Genome annotation databases

EnsembliENSRNOT00000019687; ENSRNOP00000019687; ENSRNOG00000014689.
GeneIDi287441.
KEGGirno:287441.
UCSCiRGD:1307184. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR07029862 Genomic DNA. No translation available.
RefSeqiNP_001291281.1. NM_001304352.1.
XP_006220767.1. XM_006220705.1.
XP_006220768.1. XM_006220706.2.
XP_006246906.1. XM_006246844.2.
UniGeneiRn.15836.
Rn.232400.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019687.

Proteomic databases

PaxDbiD4A8X0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019687; ENSRNOP00000019687; ENSRNOG00000014689.
GeneIDi287441.
KEGGirno:287441.
UCSCiRGD:1307184. rat.

Organism-specific databases

CTDi57659.
RGDi1307184. Zbtb4.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00810000125416.
InParanoidiD4A8X0.
KOiK10491.
OMAiNMVLPDE.
OrthoDBiEOG091G01R6.
PhylomeDBiD4A8X0.
TreeFamiTF333100.

Miscellaneous databases

PROiD4A8X0.

Gene expression databases

BgeeiENSRNOG00000014689.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 2 hits.
PROSITEiPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZBTB4_RAT
AccessioniPrimary (citable) accession number: D4A8X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: April 20, 2010
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.