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Protein

Brefeldin A-inhibited guanine nucleotide-exchange protein 1

Gene

Arfgef1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).By similarity

Enzyme regulationi

Inhibited by brefeldin A.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Short name:
BIG1
Short name:
Brefeldin A-inhibited GEP 1
Alternative name(s):
ADP-ribosylation factor guanine nucleotide-exchange factor 1
Gene namesi
Name:Arfgef1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1560793. Arfgef1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18461846Brefeldin A-inhibited guanine nucleotide-exchange protein 1PRO_0000419331Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521PhosphoserineBy similarity
Modified residuei234 – 2341PhosphoserineBy similarity
Modified residuei243 – 2431PhosphoserineBy similarity
Modified residuei269 – 2691PhosphothreonineBy similarity
Modified residuei286 – 2861PhosphoserineCombined sources
Modified residuei289 – 2891PhosphoserineCombined sources
Modified residuei290 – 2901PhosphoserineCombined sources
Modified residuei393 – 3931PhosphoserineBy similarity
Modified residuei394 – 3941PhosphoserineBy similarity
Modified residuei407 – 4071PhosphoserineBy similarity
Modified residuei661 – 6611PhosphoserineBy similarity
Modified residuei664 – 6641PhosphoserineBy similarity
Modified residuei678 – 6781PhosphothreonineBy similarity
Modified residuei1076 – 10761PhosphoserineBy similarity
Modified residuei1563 – 15631PhosphoserineCombined sources
Modified residuei1566 – 15661PhosphoserineCombined sources
Modified residuei1590 – 15901PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiD4A631.
PeptideAtlasiD4A631.
PRIDEiD4A631.

PTM databases

iPTMnetiD4A631.
PhosphoSiteiD4A631.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005703.
GenevisibleiD4A631. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with ARFGEF2/BIG2; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2. Interacts with MYO9B. Interacts with PRKAR1A and PRKAR2A. Interacts with PPP1CC. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA. Interacts with DPY30. Interacts with PDE3A. Interacts with KANK1. Interacts with TBC1D22A and TBC1D22B.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Myo9bQ633584EBI-6251168,EBI-6251137

GO - Molecular functioni

Protein-protein interaction databases

BioGridi260275. 1 interaction.
IntActiD4A631. 3 interactions.
STRINGi10116.ENSRNOP00000007766.

Structurei

3D structure databases

ProteinModelPortaliD4A631.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini688 – 877190SEC7PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 224223DCB; DCB:DCB domain and DCB:HUS domain interactionBy similarityAdd
BLAST
Regioni554 – 57421HUS; DCB:HUS domain interactionBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi708 – 7125Nuclear localization signal (NLS)By similarity

Sequence similaritiesi

Contains 1 SEC7 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0929. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
InParanoidiD4A631.
KOiK18442.
OMAiHEPESPH.
OrthoDBiEOG091G00F0.
PhylomeDBiD4A631.
TreeFamiTF300714.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032629. DCB_dom.
IPR023394. Sec7_alpha_orthog.
IPR015403. Sec7_C.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF16213. DCB. 1 hit.
PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D4A631-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKVETEK
60 70 80 90 100
QSPPHGEAKA GSGTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL
110 120 130 140 150
DCLQKLIAYG HLTGSAPDST TPGKKLIDRI IETICGCFQG PQTDEGVQLQ
160 170 180 190 200
IIKALLTAVT SQHIEIHEGT VLQAVRTCYN IYLASKNLIN QTTAKATLTQ
210 220 230 240 250
MLNVIFARME NQALQEAKQM ERERHRQQHH LLQSPVSHHE PESPHLRYLP
260 270 280 290 300
PQTVDHIAQE QEGDLDPQTH DVDKSLQDDI EPENGSDISS AENEQTEADQ
310 320 330 340 350
ATAAETLSKD DVLCDGECEE KPQDIVQSIV EEMVDIIVGD MGEGTAVSAS
360 370 380 390 400
ADGNAGAVED GSDSENVQAN GIPGTPISAA YTPSLPDDRL SVSSNDTQES
410 420 430 440 450
GNSSGPSPGA KFSHILQKDA FLVFRSLCKL SMKPLSDGPP DPKSHELRSK
460 470 480 490 500
ILSLQLLLSI LQNAGPVFRT NEMFINAIKQ YLCVALSKNG VSSVPEVFEL
510 520 530 540 550
SLSIFLTLLS NFKTHLKMQI EVFFKEIFLY ILETSTSSFD HKWMVIQTLT
560 570 580 590 600
RICADAQSVV DIYVNYDCDL NAANIFERLV NDLSKIAQGR GSQELGMSNV
610 620 630 640 650
QELSLRKKGL ECLVSILKCM VEWSKDQYVN PNSQTTLGQE KPSEQEISEI
660 670 680 690 700
KHPETINRYG SLNSLESTSS SGIGSYSTQM SGTDNPEQFE VLKQQKEIIE
710 720 730 740 750
QGIDLFNKKP KRGIQYLQEQ GMLGTTPEDI AQFLHQEERL DSTQAGEFLG
760 770 780 790 800
DNDKFNKEVM YAYVDQHDFS GKDFVSALRL FLEGFRLPGE AQKIDRLMEK
810 820 830 840 850
FAARYLECNQ GQTLFASADT AYVLAYSIIM LTTDLHSPQV KNKMTKEQYI
860 870 880 890 900
KMNRGINDSK DLPEEYLSAI YNEIAGKKIS MKETKELTIP TKSTKQNVAS
910 920 930 940 950
EKQRRLLYNL EMEQMAKTAK ALMEAVSHVQ APFTSATHLE HVRPMFKLAW
960 970 980 990 1000
TPFLAAFSVG LQDCDDTDVA SLCLEGIRCA IRIACIFSIQ LERDAYVQAL
1010 1020 1030 1040 1050
ARFTLLTVSS GITEMKQKNI DTIKTLITVA HTDGNYLGNS WHEILKCISQ
1060 1070 1080 1090 1100
LELAQLIGTG VKPRYISGTV RGREGSLTGT KDQAPDEFVG LGLVGGNVDW
1110 1120 1130 1140 1150
KQIASIQESI GETSSQSVVV AVDRIFTGST RLDGNAIVDF VRWLCAVSMD
1160 1170 1180 1190 1200
ELLSTTHPRM FSLQKIVEIS YYNMGRIRLQ WSRIWEVIGD HFNKVGCNPN
1210 1220 1230 1240 1250
EDVAIFAVDS LRQLSMKFLE KGELANFRFQ KDFLRPFEHI MKRNRSPTIR
1260 1270 1280 1290 1300
DMVVRCIAQM VNSQAANIRS GWKNIFSVFH LAASDQDESI VELAFQTSGH
1310 1320 1330 1340 1350
IVTLVFEKHF PATIDSFQDA VKCLSEFACN AAFPDTSMEA IRLIRHCAKY
1360 1370 1380 1390 1400
VSDRPQAFKE YTSDDMNVAP EDRVWVRGWF PILFELSCVI NRCKLDVRTR
1410 1420 1430 1440 1450
GLTVMFEIMK TYGHTYEKHW WQDLFRIVFR IFDNMKLPEQ QTEKAEWMTT
1460 1470 1480 1490 1500
TCNHALYAIC DVFTQYLEVL SDVLLDDIFA QLYWCVQQDN EQLARSGTNC
1510 1520 1530 1540 1550
LENVVILNGE KFTLEIWDKT CNCTLDIFKT TIPHALLTWR PTSGEAAPPS
1560 1570 1580 1590 1600
PSAMSEKQLD AISQKSVDIH DSAQPRSSDN RQQAPLVSVS PASEEVSKGR
1610 1620 1630 1640 1650
PTAKFPEQKL FAALLIKCVV QLELIQTIDN IVFFPATSKK EDAENLAAAQ
1660 1670 1680 1690 1700
RDAVDFDVRV DTQDQGMYRF LTSQQLFKLL DCLLESHRFA KAFNSNNEQR
1710 1720 1730 1740 1750
TALWKAGFKG KSKPNLLKQE TSSLACGLRI LFRMYTDESR VSAWEEVQQR
1760 1770 1780 1790 1800
LLNVCSEALS YFLTLTSESH REAWTNLLLL FLTKVLKISD NRFKAHASFY
1810 1820 1830 1840
YPLLCEIMQF DLIPELRAVL RRFFLRIGIV FQISQPPEQE LGINKQ
Length:1,846
Mass (Da):207,890
Last modified:April 20, 2010 - v1
Checksum:i3C87F6050FAC8E7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473984 Genomic DNA. Translation: EDM11544.1.
RefSeqiNP_001263985.1. NM_001277056.1.
XP_008761719.1. XM_008763497.1.
UniGeneiRn.6834.

Genome annotation databases

EnsembliENSRNOT00000007766; ENSRNOP00000007766; ENSRNOG00000005703.
GeneIDi312915.
KEGGirno:312915.
UCSCiRGD:1560793. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473984 Genomic DNA. Translation: EDM11544.1.
RefSeqiNP_001263985.1. NM_001277056.1.
XP_008761719.1. XM_008763497.1.
UniGeneiRn.6834.

3D structure databases

ProteinModelPortaliD4A631.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi260275. 1 interaction.
IntActiD4A631. 3 interactions.
STRINGi10116.ENSRNOP00000007766.

PTM databases

iPTMnetiD4A631.
PhosphoSiteiD4A631.

Proteomic databases

PaxDbiD4A631.
PeptideAtlasiD4A631.
PRIDEiD4A631.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007766; ENSRNOP00000007766; ENSRNOG00000005703.
GeneIDi312915.
KEGGirno:312915.
UCSCiRGD:1560793. rat.

Organism-specific databases

CTDi10565.
RGDi1560793. Arfgef1.

Phylogenomic databases

eggNOGiKOG0929. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
InParanoidiD4A631.
KOiK18442.
OMAiHEPESPH.
OrthoDBiEOG091G00F0.
PhylomeDBiD4A631.
TreeFamiTF300714.

Miscellaneous databases

PROiD4A631.

Gene expression databases

BgeeiENSRNOG00000005703.
GenevisibleiD4A631. RN.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032629. DCB_dom.
IPR023394. Sec7_alpha_orthog.
IPR015403. Sec7_C.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF16213. DCB. 1 hit.
PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIG1_RAT
AccessioniPrimary (citable) accession number: D4A631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: April 20, 2010
Last modified: September 7, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.