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D4A5U3

- TGM3_RAT

UniProt

D4A5U3 - TGM3_RAT

Protein

Protein-glutamine gamma-glutamyltransferase E

Gene

Tgm3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath By similarity.By similarity

    Catalytic activityi

    Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation

    Cofactori

    Binds 3 calcium ions per subunit. Binds 1 calcium ion as a zymogen, and binds 2 more calcium ions, or other divalent metal cations, after proteolytic processing By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi222 – 2221Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi225 – 2251Calcium 1By similarity
    Metal bindingi227 – 2271Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi228 – 2281Calcium 1By similarity
    Active sitei273 – 2731PROSITE-ProRule annotation
    Metal bindingi302 – 3021Calcium 2By similarity
    Metal bindingi304 – 3041Calcium 2By similarity
    Metal bindingi306 – 3061Calcium 2By similarity
    Metal bindingi308 – 3081Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi325 – 3251Calcium 2By similarity
    Active sitei331 – 3311PROSITE-ProRule annotation
    Active sitei354 – 3541PROSITE-ProRule annotation
    Metal bindingi394 – 3941Calcium 3By similarity
    Metal bindingi416 – 4161Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi444 – 4441Calcium 3By similarity
    Metal bindingi449 – 4491Calcium 3By similarity
    Sitei467 – 4682Cleavage; by CTSLBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. GDP binding Source: Ensembl
    3. GTPase activity Source: Ensembl
    4. GTP binding Source: Ensembl
    5. magnesium ion binding Source: Ensembl
    6. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. cell envelope organization Source: Ensembl
    2. keratinization Source: UniProtKB-KW
    3. peptide cross-linking Source: UniProtKB
    4. protein tetramerization Source: Ensembl

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Keratinization

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-glutamine gamma-glutamyltransferase E (EC:2.3.2.13)
    Alternative name(s):
    Transglutaminase E
    Short name:
    TG(E)
    Short name:
    TGE
    Short name:
    TGase E
    Transglutaminase-3
    Short name:
    TGase-3
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Tgm3
    Synonyms:Tgase3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi1561831. Tgm3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. extrinsic component of cytoplasmic side of plasma membrane Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 467466Protein-glutamine gamma-glutamyltransferase E 50 kDa catalytic chainPRO_0000408951Add
    BLAST
    Chaini468 – 693226Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chainPRO_0000408952Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei111 – 1111PhosphotyrosineBy similarity
    Modified residuei112 – 1121PhosphothreonineBy similarity

    Post-translational modificationi

    Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Zymogen

    Interactioni

    Subunit structurei

    Consists of two polypeptide chains, which are synthesized as a precursor form of a single polypeptide.By similarity

    Protein-protein interaction databases

    BioGridi265783. 1 interaction.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00740000115034.
    KOiK05620.
    OMAiEILPTRS.
    OrthoDBiEOG7WT40M.
    PhylomeDBiD4A5U3.
    TreeFamiTF324278.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view]
    PANTHERiPTHR11590. PTHR11590. 1 hit.
    PfamiPF00927. Transglut_C. 2 hits.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
    SMARTiSM00460. TGc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    D4A5U3-1 [UniParc]FASTAAdd to Basket

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    MSALEVQNIN WQMPMNRRAH HTDKFSSQDF IVRRGQPWEV ILLCNRSLES    50
    GDNLNFIVST GPQPSESART KAVFSISGRN TSGWSAALKA SNGNNLFIAI 100
    ASPVSAPIGL YTLNVEVSSK GRVSSVKLGT FTVLFNPWQQ GDDVFMSNHA 150
    ERQEYVEEDS GIIYVGSTNR IGMVGWNFGQ FEEDILSISL SILDRSLNFR 200
    RDPATDVARR NDPKYVCRVL SAMINANDDS GVLSGNWSGN YSGGVDPRTW 250
    NGSVEILKNW KKSGFRPVQF GQCWVFAGTL NTVLRCLGVP SRVITNFNSA 300
    HDTDRNLSVD VYYDAMGNPL EKGSDSVWNF HVWNEGWFVR TDLGPSYNGW 350
    QVLDATPQER SQGVFQCGPA SVNAIKDGEV DQNFDMIFIF AEVNADRITW 400
    IYNNRDGSQK QNSVDTYSIG KYISTKAVGS NSRMDVTIKY KHPEGSKEER 450
    QVQQKAMNKL KPNASFGATS SRGPQGEEKE PSISGKFKVT GVLAVGKEVS 500
    LALILKNTTS DRKTVTTNMT AWTIVYNGTL VHEVWKDSAT ISLDPEEEIQ 550
    YPVKIAYSQY DRYLKADNMI RITAVCKVPD EAEVVVERDV ILDNPTLTLE 600
    VLDQAQLRKP VVVQMLFSNP LDEPVKNCVL MVEGSGLLRG SLKIDVPALR 650
    PKEKSRVRFE IFPTRIGIKQ LLADFSCNKF PAIKAMLVIE VSE 693
    Length:693
    Mass (Da):77,230
    Last modified:April 20, 2010 - v1
    Checksum:iCFE4D1B46960D4C2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH473949 Genomic DNA. Translation: EDL80172.1.
    RefSeqiNP_001102429.1. NM_001108959.1.
    UniGeneiRn.53317.

    Genome annotation databases

    EnsembliENSRNOT00000063828; ENSRNOP00000059160; ENSRNOG00000006753.
    GeneIDi366189.
    KEGGirno:366189.
    UCSCiRGD:1561831. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH473949 Genomic DNA. Translation: EDL80172.1 .
    RefSeqi NP_001102429.1. NM_001108959.1.
    UniGenei Rn.53317.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 265783. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000063828 ; ENSRNOP00000059160 ; ENSRNOG00000006753 .
    GeneIDi 366189.
    KEGGi rno:366189.
    UCSCi RGD:1561831. rat.

    Organism-specific databases

    CTDi 7053.
    RGDi 1561831. Tgm3.

    Phylogenomic databases

    GeneTreei ENSGT00740000115034.
    KOi K05620.
    OMAi EILPTRS.
    OrthoDBi EOG7WT40M.
    PhylomeDBi D4A5U3.
    TreeFami TF324278.

    Miscellaneous databases

    NextBioi 688908.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProi IPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view ]
    PANTHERi PTHR11590. PTHR11590. 1 hit.
    Pfami PF00927. Transglut_C. 2 hits.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000459. TGM_EBP42. 1 hit.
    SMARTi SM00460. TGc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiTGM3_RAT
    AccessioniPrimary (citable) accession number: D4A5U3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3