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Protein

Beclin 1-associated autophagy-related key regulator

Gene

Atg14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-RNO-1632852 Macroautophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Beclin 1-associated autophagy-related key regulatorBy similarity
Short name:
BarkorBy similarity
Alternative name(s):
Autophagy-related protein 14-like proteinBy similarity
Short name:
Atg14LBy similarity
Gene namesi
Name:Atg14By similarity
Synonyms:Atg14LBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi1304610 Atg14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004011941 – 492Beclin 1-associated autophagy-related key regulatorAdd BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei429PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiD4A4K3
PeptideAtlasiD4A4K3
PRIDEiD4A4K3

PTM databases

PhosphoSitePlusiD4A4K3

Expressioni

Gene expression databases

BgeeiENSRNOG00000011873
GenevisibleiD4A4K3 RN

Interactioni

Subunit structurei

Forms homooligomers; homo-oligomerization is essential for the roles in membrane tethering and enhancement of SNARE-mediated fusion. Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex I (PI3KC3-C1) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with ATG14. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. PI3KC3-C1 can associate with further regulatory subunits. Interacts with PIK3CB. Interacts (via coiled-coil domain) with BECN2 (via coiled-coil domain); this interaction is tighter than BECN2 self-association (By similarity). Interacts with the STX17-SNAP29 binary t-SNARE complex (By similarity). Interacts with NRBF2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60850N
IntActiD4A4K3, 1 interactor
STRINGi10116.ENSRNOP00000016274

Structurei

3D structure databases

SMRiD4A4K3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili70 – 180Sequence analysisAdd BLAST111

Domaini

The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.By similarity
The final 80 residues in the C-terminus define a minimum required region for autophagosome binding called BATS.By similarity
The N-terminal cysteine repeats are required for proper localization to the endoplasmic reticulum.By similarity

Sequence similaritiesi

Belongs to the ATG14 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4398 Eukaryota
ENOG410Y9W5 LUCA
GeneTreeiENSGT00390000011854
InParanoidiD4A4K3
KOiK17889
OMAiNTSYIRG
OrthoDBiEOG091G074J
PhylomeDBiD4A4K3
TreeFamiTF323392

Family and domain databases

InterProiView protein in InterPro
IPR018791 UV_resistance/autophagy_Atg14
PfamiView protein in Pfam
PF10186 Atg14, 1 hit

Sequencei

Sequence statusi: Complete.

D4A4K3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPSGKGSW TPEAPGFGPR ALAPDLVDSV DDAEGLYVAV ERCPLCNTTR
60 70 80 90 100
RRLTCAKCVQ SGDFVYFDGR DRERFIDKKE RLSQLKNKQE EFQKEVLKAM
110 120 130 140 150
EGKRLTDQLR WKIMSCKMRI EQLKQTICKG NEEMKKNSEG LLKNKEKNQK
160 170 180 190 200
LYSRAQRHQE KKEKIQRHNR KLGDLVEKKT SDLREHYDRL ACLRRLHILE
210 220 230 240 250
LTSVIFPMDE VKTSGRDPAD VSSETDSAMT SSMVSKLAEA RRTTYLSGRW
260 270 280 290 300
VCDDHNGDTS ISITGPWISL PNNGDYSAYY NWVEEKKTTQ GPDMEHNNPA
310 320 330 340 350
YTISAALGYA TQLVNIVSHI LDINLPKKLC NSEFCGENLS KQRLTRAVRK
360 370 380 390 400
LNANILYLCS SQHVNLDQLQ PLHTLRNLMH LVSPHSEHLG RSGPFEVRAD
410 420 430 440 450
LEESMEFVDP GVAGESDVSG DERVSDEETD LGTDWENLPS PRFCDIPSQP
460 470 480 490
VEVSQSQSTQ ASPPIASSSA GGMISSAAAS VTSWFKAYTG HR
Length:492
Mass (Da):55,345
Last modified:April 20, 2010 - v1
Checksum:iF7647AFDDE3E7FA6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474040 Genomic DNA Translation: EDL88360.1
RefSeqiNP_001100728.1, NM_001107258.1
UniGeneiRn.47301

Genome annotation databases

EnsembliENSRNOT00000016274; ENSRNOP00000016274; ENSRNOG00000011873
GeneIDi305831
KEGGirno:305831
UCSCiRGD:1304610 rat

Similar proteinsi

Entry informationi

Entry nameiBAKOR_RAT
AccessioniPrimary (citable) accession number: D4A4K3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: April 20, 2010
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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