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Protein

Serine/threonine-protein kinase PAK 5

Gene

Pak5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei477ATPPROSITE-ProRule annotation1
Active sitei567Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi454 – 462ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PAK 5 (EC:2.7.11.1)
Alternative name(s):
p21-activated kinase 5
Short name:
PAK-5
p21-activated kinase 7
Short name:
PAK-7
Gene namesi
Name:Pak5
Synonyms:Pak7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1312009 Pak7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004130661 – 718Serine/threonine-protein kinase PAK 5Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineBy similarity1
Modified residuei78N6-methyllysineBy similarity1
Modified residuei104PhosphoserineCombined sources1
Modified residuei107PhosphothreonineCombined sources1
Modified residuei288PhosphoserineBy similarity1
Modified residuei386PhosphoserineBy similarity1
Modified residuei395PhosphoserineBy similarity1
Modified residuei418PhosphoserineBy similarity1
Modified residuei601PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated when activated by CDC42/p21.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiD4A280
PeptideAtlasiD4A280
PRIDEiD4A280

PTM databases

iPTMnetiD4A280
PhosphoSitePlusiD4A280

Expressioni

Gene expression databases

BgeeiENSRNOG00000005509

Interactioni

Subunit structurei

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with MARK2, leading to inhibit MARK2 independently of kinase activity. Interacts with RHOD and RHOH (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007410

Structurei

3D structure databases

ProteinModelPortaliD4A280
SMRiD4A280
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 24CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini448 – 699Protein kinasePROSITE-ProRule annotationAdd BLAST252

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 447LinkerBy similarityAdd BLAST423

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 8Poly-Lys5
Compositional biasi223 – 226Poly-Ser4
Compositional biasi367 – 370Poly-Ser4

Domaini

An autoinhibitory domain is present in the N-terminal region of the protein.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0578 Eukaryota
ENOG410XP4K LUCA
GeneTreeiENSGT00910000143994
InParanoidiD4A280
KOiK05736
OMAiMRQYRHH
OrthoDBiEOG091G0OT5
PhylomeDBiD4A280
TreeFamiTF105352

Family and domain databases

CDDicd01093 CRIB_PAK_like, 1 hit
Gene3Di3.90.810.101 hit
InterProiView protein in InterPro
IPR000095 CRIB_dom
IPR036936 CRIB_dom_sf
IPR011009 Kinase-like_dom_sf
IPR028754 PAK5
IPR033923 PAK_BD
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PANTHERiPTHR24361:SF183 PTHR24361:SF183, 1 hit
PfamiView protein in Pfam
PF00786 PBD, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00285 PBD, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50108 CRIB, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

D4A280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGKKKKKIE ISGPSNFEHR VHTGFDPQEQ KFTGLPQQWH SLLADTANRP
60 70 80 90 100
KPMVDPSCIT PIQLAPMKTI VRGNKSCKES SINGLLEDFD NISVTRSNSL
110 120 130 140 150
RKESPPTPDQ GAASRIQGHS EENGFITFSQ YSSESDTTTD YTTEKYRDRS
160 170 180 190 200
LYGDDLDLYY RGSHAAKQNG HAMKMKHGDA YYPEMKPLKS DLARFPVDYH
210 220 230 240 250
THLDSLSKAS EYGDLKWDYQ RASSSSPLDY SFQLTPSRTA GTSRCSKESL
260 270 280 290 300
AYSESDWGPS FDDYDRRPKS SYLHQTSPQP AMRQRSKSGS GLQEPMMPFG
310 320 330 340 350
ASAFKTHPQG HSYNSYTYPR LSEPTMCIPK VDYDRAQMVF SPPLSGSDTY
360 370 380 390 400
PRGPTKLPQS QSKVGYSSSS HQYPGYHKAS LYHHPSLQTS SQYISTASYL
410 420 430 440 450
SSLSISSSTY PPPSWGSSSD QQPSRVSHEQ FRAALQLVVS PGDPREYLDN
460 470 480 490 500
FIKIGEGSTG IVCIATEKHT GKQVAVKKMD LRKQQRRELL FNEVVIMRDY
510 520 530 540 550
HHDNVVDMYN SYLVGDELWV VMEFLEGGAL TDIVTHTRMN EEQIATVCLS
560 570 580 590 600
VLKALSYLHN QGVIHRDIKS DSILLTSDGR IKLSDFGFCA QVSKEVPKRK
610 620 630 640 650
SLVGTPYWMA PEVISRLPYG TEVDIWSLGI MVIEMIDGEP PYFNEPPLQA
660 670 680 690 700
MRRIRDSLPP RVKDLHKVSS MLRGFLDLML VREPSQRATA QELLGHPFLK
710
LAGPPSCIVP LMRQYRHH
Length:718
Mass (Da):80,949
Last modified:April 20, 2010 - v1
Checksum:iFA48783A5813C508
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA Translation: EDL80300.1
RefSeqiNP_001101251.1, NM_001107781.1
XP_017447245.1, XM_017591756.1
XP_017447246.1, XM_017591757.1
XP_017447247.1, XM_017591758.1
UniGeneiRn.225058

Genome annotation databases

EnsembliENSRNOT00000007410; ENSRNOP00000007410; ENSRNOG00000005509
ENSRNOT00000084829; ENSRNOP00000071237; ENSRNOG00000005509
GeneIDi311450
KEGGirno:311450
UCSCiRGD:1312009 rat

Similar proteinsi

Entry informationi

Entry nameiPAK5_RAT
AccessioniPrimary (citable) accession number: D4A280
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: April 20, 2010
Last modified: April 25, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome