Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase PAK 7

Gene

Pak7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei477 – 4771ATPPROSITE-ProRule annotation
Active sitei567 – 5671Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi454 – 4629ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PAK 7 (EC:2.7.11.1)
Alternative name(s):
p21-activated kinase 5
Short name:
PAK-5
p21-activated kinase 7
Short name:
PAK-7
Gene namesi
Name:Pak7
Synonyms:Pak5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1312009. Pak7.

Subcellular locationi

  • Mitochondrion By similarity
  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Serine/threonine-protein kinase PAK 7PRO_0000413066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei104 – 1041PhosphoserineCombined sources
Modified residuei107 – 1071PhosphothreonineCombined sources

Post-translational modificationi

Autophosphorylated when activated by CDC42/p21.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiD4A280.
PeptideAtlasiD4A280.
PRIDEiD4A280.

PTM databases

iPTMnetiD4A280.
PhosphoSiteiD4A280.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005509.

Interactioni

Subunit structurei

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with MARK2, leading to inhibit MARK2 independently of kinase activity. Interacts with RHOD and RHOH (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007410.

Structurei

3D structure databases

ProteinModelPortaliD4A280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 2414CRIBPROSITE-ProRule annotationAdd
BLAST
Domaini448 – 699252Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 447423LinkerBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 85Poly-Lys
Compositional biasi223 – 2264Poly-Ser
Compositional biasi367 – 3704Poly-Ser

Domaini

An autoinhibitory domain is present in the N-terminal region of the protein.By similarity

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0578. Eukaryota.
ENOG410XP4K. LUCA.
GeneTreeiENSGT00840000129718.
InParanoidiD4A280.
KOiK05736.
OMAiNQTSPQP.
OrthoDBiEOG091G0OT5.
PhylomeDBiD4A280.
TreeFamiTF105352.

Family and domain databases

Gene3Di3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR028754. PAK7.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PANTHERiPTHR24361:SF183. PTHR24361:SF183. 2 hits.
PfamiPF00786. PBD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D4A280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGKKKKKIE ISGPSNFEHR VHTGFDPQEQ KFTGLPQQWH SLLADTANRP
60 70 80 90 100
KPMVDPSCIT PIQLAPMKTI VRGNKSCKES SINGLLEDFD NISVTRSNSL
110 120 130 140 150
RKESPPTPDQ GAASRIQGHS EENGFITFSQ YSSESDTTTD YTTEKYRDRS
160 170 180 190 200
LYGDDLDLYY RGSHAAKQNG HAMKMKHGDA YYPEMKPLKS DLARFPVDYH
210 220 230 240 250
THLDSLSKAS EYGDLKWDYQ RASSSSPLDY SFQLTPSRTA GTSRCSKESL
260 270 280 290 300
AYSESDWGPS FDDYDRRPKS SYLHQTSPQP AMRQRSKSGS GLQEPMMPFG
310 320 330 340 350
ASAFKTHPQG HSYNSYTYPR LSEPTMCIPK VDYDRAQMVF SPPLSGSDTY
360 370 380 390 400
PRGPTKLPQS QSKVGYSSSS HQYPGYHKAS LYHHPSLQTS SQYISTASYL
410 420 430 440 450
SSLSISSSTY PPPSWGSSSD QQPSRVSHEQ FRAALQLVVS PGDPREYLDN
460 470 480 490 500
FIKIGEGSTG IVCIATEKHT GKQVAVKKMD LRKQQRRELL FNEVVIMRDY
510 520 530 540 550
HHDNVVDMYN SYLVGDELWV VMEFLEGGAL TDIVTHTRMN EEQIATVCLS
560 570 580 590 600
VLKALSYLHN QGVIHRDIKS DSILLTSDGR IKLSDFGFCA QVSKEVPKRK
610 620 630 640 650
SLVGTPYWMA PEVISRLPYG TEVDIWSLGI MVIEMIDGEP PYFNEPPLQA
660 670 680 690 700
MRRIRDSLPP RVKDLHKVSS MLRGFLDLML VREPSQRATA QELLGHPFLK
710
LAGPPSCIVP LMRQYRHH
Length:718
Mass (Da):80,949
Last modified:April 20, 2010 - v1
Checksum:iFA48783A5813C508
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL80300.1.
RefSeqiNP_001101251.1. NM_001107781.1.
UniGeneiRn.225058.

Genome annotation databases

EnsembliENSRNOT00000007410; ENSRNOP00000007410; ENSRNOG00000005509.
ENSRNOT00000084829; ENSRNOP00000071237; ENSRNOG00000005509.
GeneIDi311450.
KEGGirno:311450.
UCSCiRGD:1312009. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473949 Genomic DNA. Translation: EDL80300.1.
RefSeqiNP_001101251.1. NM_001107781.1.
UniGeneiRn.225058.

3D structure databases

ProteinModelPortaliD4A280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007410.

PTM databases

iPTMnetiD4A280.
PhosphoSiteiD4A280.

Proteomic databases

PaxDbiD4A280.
PeptideAtlasiD4A280.
PRIDEiD4A280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007410; ENSRNOP00000007410; ENSRNOG00000005509.
ENSRNOT00000084829; ENSRNOP00000071237; ENSRNOG00000005509.
GeneIDi311450.
KEGGirno:311450.
UCSCiRGD:1312009. rat.

Organism-specific databases

CTDi57144.
RGDi1312009. Pak7.

Phylogenomic databases

eggNOGiKOG0578. Eukaryota.
ENOG410XP4K. LUCA.
GeneTreeiENSGT00840000129718.
InParanoidiD4A280.
KOiK05736.
OMAiNQTSPQP.
OrthoDBiEOG091G0OT5.
PhylomeDBiD4A280.
TreeFamiTF105352.

Miscellaneous databases

PROiD4A280.

Gene expression databases

BgeeiENSRNOG00000005509.

Family and domain databases

Gene3Di3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR028754. PAK7.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PANTHERiPTHR24361:SF183. PTHR24361:SF183. 2 hits.
PfamiPF00786. PBD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAK7_RAT
AccessioniPrimary (citable) accession number: D4A280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: April 20, 2010
Last modified: September 7, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.