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Protein

Protein-methionine sulfoxide oxidase MICAL2

Gene

Mical2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin and regulates the SRF signaling. Acts by modifying nuclear actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA (By similarity).By similarity

Catalytic activityi

[protein]-methionine + NADPH + O2 = [protein]-methionine-sulfoxide + NADP+ + H2O.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971FADBy similarity
Binding sitei116 – 1161FADBy similarity
Binding sitei118 – 1181FADBy similarity
Binding sitei123 – 1231FADBy similarity
Binding sitei125 – 1251FADBy similarity
Binding sitei398 – 3981FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi97 – 12529FADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Actin-binding, FAD, Flavoprotein, Metal-binding, NADP, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-methionine sulfoxide oxidase MICAL2 (EC:1.14.13.-)
Alternative name(s):
Molecule interacting with CasL protein 2
Short name:
MICAL-2
Gene namesi
Name:Mical2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1311773. Mical2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961Protein-methionine sulfoxide oxidase MICAL2PRO_0000416302Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei631 – 6311PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiD4A1F2.
PeptideAtlasiD4A1F2.
PRIDEiD4A1F2.

PTM databases

iPTMnetiD4A1F2.
PhosphoSiteiD4A1F2.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016244.

Interactioni

Subunit structurei

Interacts with VIM and PLXNA4. Interacts with RAB1B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi265309. 1 interaction.
STRINGi10116.ENSRNOP00000021858.

Structurei

3D structure databases

ProteinModelPortaliD4A1F2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini516 – 619104CHPROSITE-ProRule annotationAdd
BLAST
Domaini752 – 81463LIM zinc-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 494493Monooxygenase domainBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi660 – 68122Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the Mical family.Curated
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain

Phylogenomic databases

eggNOGiKOG1700. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118856.
InParanoidiD4A1F2.
KOiK19947.
OMAiCSVCATT.
PhylomeDBiD4A1F2.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
2.10.110.10. 1 hit.
3.50.50.60. 1 hit.
InterProiIPR001715. CH-domain.
IPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF01494. FAD_binding_3. 1 hit.
PF00412. LIM. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00132. LIM. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiPS50021. CH. 1 hit.
PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: D4A1F2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGENEDEKQA QASQVFENFV QATTCKGTLQ AFNILTCLLD LDPLDHRNFY
60 70 80 90 100
TQLKSKVNTW KAKALWHKLD KRGSHKEYKR GKACSNTKCL IVGGGPCGLR
110 120 130 140 150
TAIELAYLGA KVVVVEKRDT FSRNNVLHLW PFTIHDLRGL GAKKFYGKFC
160 170 180 190 200
AGSIDHISIR QLQLILFKVA LMLGVEIHVN VEFVRVREPP KDQENRKIGW
210 220 230 240 250
RAEFLPADHA LSNFEFDVII GADGHRNTLE GFRRKEFRGK LAIAITANFI
260 270 280 290 300
NRNSTAEAKV EEISGVAFIF NQKFFQDLKE ETGIDLENIV YYKDSTHYFV
310 320 330 340 350
MTAKKQSLLD KGVILNDYID TEMLLCAENV NQDNLLSYAR EAADFATNYQ
360 370 380 390 400
LPSLDFAINH NGQPDVAMFD FTSMYASENA ALMRERQAHQ LLVALVGDSL
410 420 430 440 450
LEPFWPMGTG CARGFLAAFD TAWMVKSWDQ GTPPLEVLAE RESLYRLLPQ
460 470 480 490 500
TTPENINKNF EQYTLDPATR YPNLNVHCVR PHQVKHLYIT KEMDRFPLER
510 520 530 540 550
WGSVRRSASL SRRESDIRPN KLLTWCQQQT KGYQHVRVTD LTTSWRSGLA
560 570 580 590 600
LCAIIHSFRP ELINFDSLNE NDVVENNQLA FDVAKREFGI LPVTTGKEMA
610 620 630 640 650
STQEPDKLSM VMYLSKFYEL FRGTPLRPMD SRRKNYGENA DFGLGKTFFQ
660 670 680 690 700
NNYLNLTLPR KRTPRVDAQT EENDVNKRRR QGFNNLEELP AFSSRSLGSS
710 720 730 740 750
QEYAKESGNQ NKVKYMANQL LAKFEENTRN PSALKQESLR KAEFPLSLGG
760 770 780 790 800
RDTCYFCKKR VYVMERLSAE GHFFHRECFR CSVCAAILRV AAYAFDCDEG
810 820 830 840 850
KFYCKLHFAH CKTSSKQRKR RAELNQQREE EGTWPEQEAA RRDVPAESSC
860 870 880 890 900
AVAAISTPEG SPPGISTSFF RKALSWPLRL TRGLLNLPQS LLRWMQGLLQ
910 920 930 940 950
AAGHHVRDNA HNYCFMFELL SLGLLLLWAF SEVLAAMYRE SEESLESIRS
960
WLLRFVPVKL Q
Length:961
Mass (Da):110,093
Last modified:April 20, 2010 - v1
Checksum:i450CA9CC95037905
GO
Isoform 2 (identifier: D4A1F2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     736-736: Q → QDCPRVSGMG...PKTQATSPDL
     864-886: GISTSFFRKALSWPLRLTRGLLN → VRFSLPVLHPLLG
     887-961: Missing.

Show »
Length:1,103
Mass (Da):124,185
Checksum:i28B7AAFBE6D87E57
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei736 – 7361Q → QDCPRVSGMGKPVLCSASRP PGTSHCPKLEESTPRLPPPL KRQFSSTVATGQVLRELNQV PASGECPGRPWRARAKSDLQ LGGAENLATLPPTCQGALAL SGVLRRLQQVEEKVLQKRAQ NLANREFHTKNIKEKAAHLA SMFGHGDLPQDKLLSKRVPH AHPPSPPSCLPSPDPAAAPS PPAADSVSPARKLTVGKVSS GIGAAAEVLVNLYLNDHRPK TQATSPDL in isoform 2. CuratedVSP_042609
Alternative sequencei864 – 88623GISTS…RGLLN → VRFSLPVLHPLLG in isoform 2. CuratedVSP_042610Add
BLAST
Alternative sequencei887 – 96175Missing in isoform 2. CuratedVSP_042611Add
BLAST

Sequence databases

RefSeqiNP_001132980.1. NM_001139508.1. [D4A1F2-1]
XP_006230127.1. XM_006230065.2. [D4A1F2-2]
XP_006230128.1. XM_006230066.2. [D4A1F2-2]
UniGeneiRn.24515.

Genome annotation databases

EnsembliENSRNOT00000021858; ENSRNOP00000021858; ENSRNOG00000016244. [D4A1F2-1]
ENSRNOT00000081595; ENSRNOP00000074877; ENSRNOG00000016244. [D4A1F2-2]
GeneIDi365352.
KEGGirno:365352.
UCSCiRGD:1311773. rat.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

RefSeqiNP_001132980.1. NM_001139508.1. [D4A1F2-1]
XP_006230127.1. XM_006230065.2. [D4A1F2-2]
XP_006230128.1. XM_006230066.2. [D4A1F2-2]
UniGeneiRn.24515.

3D structure databases

ProteinModelPortaliD4A1F2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi265309. 1 interaction.
STRINGi10116.ENSRNOP00000021858.

PTM databases

iPTMnetiD4A1F2.
PhosphoSiteiD4A1F2.

Proteomic databases

PaxDbiD4A1F2.
PeptideAtlasiD4A1F2.
PRIDEiD4A1F2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021858; ENSRNOP00000021858; ENSRNOG00000016244. [D4A1F2-1]
ENSRNOT00000081595; ENSRNOP00000074877; ENSRNOG00000016244. [D4A1F2-2]
GeneIDi365352.
KEGGirno:365352.
UCSCiRGD:1311773. rat.

Organism-specific databases

CTDi9645.
RGDi1311773. Mical2.

Phylogenomic databases

eggNOGiKOG1700. Eukaryota.
COG5069. LUCA.
GeneTreeiENSGT00760000118856.
InParanoidiD4A1F2.
KOiK19947.
OMAiCSVCATT.
PhylomeDBiD4A1F2.

Miscellaneous databases

PROiD4A1F2.

Gene expression databases

BgeeiENSRNOG00000016244.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
2.10.110.10. 1 hit.
3.50.50.60. 1 hit.
InterProiIPR001715. CH-domain.
IPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF01494. FAD_binding_3. 1 hit.
PF00412. LIM. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00132. LIM. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiPS50021. CH. 1 hit.
PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMICA2_RAT
AccessioniPrimary (citable) accession number: D4A1F2
Secondary accession number(s): F1LW55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: April 20, 2010
Last modified: September 7, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.