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Protein

Proline-rich membrane anchor 1

Gene

Prima1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required to anchor acetylcholinesterase (ACHE) to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses in brain. Organizes ACHE into tetramers (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processNeurotransmitter degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-rich membrane anchor 1
Short name:
PRiMA
Gene namesi
Name:Prima1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1583978 Prima1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 92ExtracellularSequence analysisAdd BLAST57
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 153CytoplasmicSequence analysisAdd BLAST40

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000041072236 – 153Proline-rich membrane anchor 1Add BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiD3ZZP4

Expressioni

Tissue specificityi

Isoforms 1 and 2 are expressed in the adult brain. In matured cortical neurons, only isoform 1 is detectable.2 Publications

Developmental stagei

Up-regulated during the differentiation of in vitro cultured cortical neurons.

Gene expression databases

BgeeiENSRNOG00000008915

Interactioni

Subunit structurei

Interacts with ACHE, probably through disulfide bonds.1 Publication

Structurei

3D structure databases

SMRiD3ZZP4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 70PRADAdd BLAST15

Domaini

The proline-rich attachment domain (PRAD) binds the AChE catalytic subunits.By similarity

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZWQ Eukaryota
ENOG410YWDZ LUCA
GeneTreeiENSGT00390000006240
InParanoidiD3ZZP4
OMAiRRGCCWP
OrthoDBiEOG091G0P7D
TreeFamiTF337232

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: D3ZZP4-1) [UniParc]FASTAAdd to basket
Also known as: Variant I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLRDLVLRH GCCWPSLLLH CALHPLWGLV QVTHAEPQKS CSKVTDSCQH
60 70 80 90 100
ICQCRPPPPL PPPPPPPPPP RLLSAPAPNS TSCPAEDSWW SGLVIIVAVV
110 120 130 140 150
CASLVFLTVL VIICYKAIKR KPLRKDENGA SVAEYPMSSS PSNKGVDVNA

AVV
Length:153
Mass (Da):16,495
Last modified:April 20, 2010 - v1
Checksum:iF3B330ACBD5A28C6
GO
Isoform 2 (identifier: D3ZZP4-2) [UniParc]FASTAAdd to basket
Also known as: Variant II

The sequence of this isoform differs from the canonical sequence as follows:
     121-124: KPLR → NQAI
     125-153: Missing.

Note: Gene prediction based on similarity to human and mouse ortholog. No experimental confirmation available.
Show »
Length:124
Mass (Da):13,522
Checksum:i7313DEB8D27972C2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041497121 – 124KPLR → NQAI in isoform 2. Curated4
Alternative sequenceiVSP_041498125 – 153Missing in isoform 2. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM029865 mRNA No translation available.
RefSeqiNP_001102191.2, NM_001108721.2 [D3ZZP4-1]
XP_006240557.1, XM_006240495.3 [D3ZZP4-1]
UniGeneiRn.152596

Genome annotation databases

EnsembliENSRNOT00000011906; ENSRNOP00000011906; ENSRNOG00000008915 [D3ZZP4-1]
GeneIDi690195
KEGGirno:690195

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPRIMA_RAT
AccessioniPrimary (citable) accession number: D3ZZP4
Secondary accession number(s): D4ACH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: April 20, 2010
Last modified: May 23, 2018
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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