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Protein

N-acetylaspartate synthetase

Gene

Nat8l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake (By similarity).By similarity

Catalytic activityi

Acetyl-CoA + L-aspartate = CoA + N-acetyl-L-aspartate.

Enzyme regulationi

Aminooxyacetic acid (AOAA) blocks its activity in both cytoplasm and mitochondria.By similarity

GO - Molecular functioni

  • aspartate N-acetyltransferase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylaspartate synthetase
Short name:
NAA synthetase
Alternative name(s):
N-acetyltransferase 8-like protein (EC:2.3.1.17)
Gene namesi
Name:Nat8l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1305719. Nat8l.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299N-acetylaspartate synthetasePRO_0000406989Add
BLAST

Proteomic databases

PaxDbiD3ZVU9.
PRIDEiD3ZVU9.

Expressioni

Tissue specificityi

Expressed in brain, including in mesencephalic dopaminergic neurons of the substantia nigra and ventral tegmental area and oligodendrocytes. Expressed in cortical pyramidal neurons and granule cells of the hippocampus (at protein level).1 Publication

Gene expression databases

GenevisibleiD3ZVU9. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066413.

Structurei

3D structure databases

ProteinModelPortaliD3ZVU9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 280138N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the camello family.Curated
Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3139. Eukaryota.
COG0456. LUCA.
GeneTreeiENSGT00390000009559.
InParanoidiD3ZVU9.
KOiK18309.
OMAiPDMVCET.
OrthoDBiEOG73Z2W2.
PhylomeDBiD3ZVU9.
TreeFamiTF324687.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3ZVU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCGPPDMVC ETKIVATEDH EALPGAKKDA LLAAAGAMWP PLPAAPGPAA
60 70 80 90 100
APPPAAGPQP HGGTGGAGPP EGRGVCIREF RAAEQEAARR IFYDGILERI
110 120 130 140 150
PNTAFRGLRQ HPRTQLLYAL LAALCFAVTR SLLLTCLVPA GLLALRYYYS
160 170 180 190 200
RKVILAYLEC ALHTDMADIE QYYMKPPGSC FWVAVLDGNV VGIVAARAHE
210 220 230 240 250
EDNTVELLRM SVDSRFRGKG IAKALGRRVL EFAMLHNYSA VVLGTTAVKV
260 270 280 290
AAHKLYESLG FRHMGASDHY VLPGMTLSLA ERLFFQVRYH RYRLQLREE
Length:299
Mass (Da):32,719
Last modified:April 20, 2010 - v1
Checksum:i4F971F8FFBE65780
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473963 Genomic DNA. Translation: EDM00108.1.
RefSeqiNP_001178610.1. NM_001191681.1.
UniGeneiRn.76732.

Genome annotation databases

EnsembliENSRNOT00000074734; ENSRNOP00000066413; ENSRNOG00000049351.
GeneIDi289727.
KEGGirno:289727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473963 Genomic DNA. Translation: EDM00108.1.
RefSeqiNP_001178610.1. NM_001191681.1.
UniGeneiRn.76732.

3D structure databases

ProteinModelPortaliD3ZVU9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066413.

Proteomic databases

PaxDbiD3ZVU9.
PRIDEiD3ZVU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000074734; ENSRNOP00000066413; ENSRNOG00000049351.
GeneIDi289727.
KEGGirno:289727.

Organism-specific databases

CTDi339983.
RGDi1305719. Nat8l.

Phylogenomic databases

eggNOGiKOG3139. Eukaryota.
COG0456. LUCA.
GeneTreeiENSGT00390000009559.
InParanoidiD3ZVU9.
KOiK18309.
OMAiPDMVCET.
OrthoDBiEOG73Z2W2.
PhylomeDBiD3ZVU9.
TreeFamiTF324687.

Miscellaneous databases

NextBioi630220.
PROiD3ZVU9.

Gene expression databases

GenevisibleiD3ZVU9. RN.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "N-acetylaspartate synthesis in the brain: mitochondria vs. microsomes."
    Ariyannur P.S., Madhavarao C.N., Namboodiri A.M.
    Brain Res. 1227:34-41(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "Methamphetamine-induced neuronal protein NAT8L is the NAA biosynthetic enzyme: implications for specialized acetyl coenzyme A metabolism in the CNS."
    Ariyannur P.S., Moffett J.R., Manickam P., Pattabiraman N., Arun P., Nitta A., Nabeshima T., Madhavarao C.N., Namboodiri A.M.
    Brain Res. 1335:1-13(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiNAT8L_RAT
AccessioniPrimary (citable) accession number: D3ZVU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: April 20, 2010
Last modified: November 11, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.