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Protein

Ubiquitinyl hydrolase 1

Gene

Usp5

Organism
Rattus norvegicus (Rat)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei209SubstrateUniRule annotation1
Binding sitei259SubstrateUniRule annotation1
Binding sitei261Substrate; via carbonyl oxygenUniRule annotation1
Binding sitei264SubstrateUniRule annotation1
Active sitei335NucleophileUniRule annotation1
Active sitei818Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol proteaseSAAS annotation
Biological processUbl conjugation pathwaySAAS annotation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-5689880. Ub-specific processing proteases.
R-RNO-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Protein family/group databases

MEROPSiC19.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitinyl hydrolase 1SAAS annotation (EC:3.4.19.12SAAS annotation)
Gene namesi
Name:Usp5Imported
Synonyms:Usp5_predictedImported
ORF Names:rCG_29800Imported
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1308438. Usp5.

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi195 ↔ 816UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation

Expressioni

Gene expression databases

BgeeiENSRNOG00000015409.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020785.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 269UBP-typeInterPro annotationAdd BLAST73
Domaini326 – 856USPInterPro annotationAdd BLAST531
Domaini654 – 695UBAInterPro annotationAdd BLAST42
Domaini722 – 762UBAInterPro annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 224Substrate bindingUniRule annotation4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili495 – 518Sequence analysisAdd BLAST24

Sequence similaritiesi

Belongs to the peptidase C19 family.SAAS annotation

Keywords - Domaini

Coiled coilSequence analysis, Zinc-fingerSAAS annotation

Phylogenomic databases

eggNOGiKOG0944. Eukaryota.
COG5207. LUCA.
GeneTreeiENSGT00390000000874.
KOiK11836.
OMAiGQRAYLH.
OrthoDBiEOG091G01W3.
TreeFamiTF300576.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR015940. UBA.
IPR009060. UBA-like.
IPR016652. Ubiquitinyl_hydrolase.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiView protein in SMART
SM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiView protein in PROSITE
PS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequencei

Sequence statusi: Complete.

D3ZVQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF
60 70 80 90 100
LGFGKQYVER HFNKTGQRVY LHLRRTRRPK EEDSSAGTGD PPRKKPTRLA
110 120 130 140 150
IGVEGGFDLT EDKFEYDEDV KIVILPDYLE IARDGLGGLP DIVRDRVTSA
160 170 180 190 200
VEALLSADSA SRKQEVQAWD GEVRQVSKHA FNLKQLDNPA RIPPCGWRCS
210 220 230 240 250
KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET GYPLAVKLGT
260 270 280 290 300
ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM
310 320 330 340 350
NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF
360 370 380 390 400
QRKYVDKLEK IFQNAPTDPT QDFSTQVAKL GHGLLSGEYS KPALESGDGE
410 420 430 440 450
QVPEQKEVQD GIAPRMFKAL IGKGHPEFST NRQQDAQEFF LHLINMVERN
460 470 480 490 500
CRSSENPNEV FRFLVEEKIK CLATEKVKYT QRVDYIMQLP VPMDAALNKE
510 520 530 540 550
ELLEYEEKKR QAEEEKVALP ELVRAQVPFS SCLEAYGAPE QVDDFWSTAL
560 570 580 590 600
QAKSVAVKTT RFASFPDYLV IQIKKFTFGL DWVPKKLDVS IEMPEELDIS
610 620 630 640 650
QLRGTGLQPG EEELPDIAPP LVTPDEPKGS LGFYGNEDED SFCSPHFSSP
660 670 680 690 700
TSPMLDESVV IQLVEMGFPM DACRKAVYYT GNSGAEAAMN WVMSHMDDPD
710 720 730 740 750
FANPLILPGS SGPGSTSAAA DPPPEDCVAT IVSMGFSRDQ ALKALRATNN
760 770 780 790 800
SLERAVDWIF SHIDDLDAEA AMDISEGRSA ADSISESVPV GPKVRDGPGK
810 820 830 840 850
YQLFAFISHM GTSTMCGHYV CHIKKEGRWV IYNDQKVCAS EKPPKDLGYI

YFYQRVVS
Length:858
Mass (Da):95,779
Last modified:April 20, 2010 - v1
Checksum:iBFC76150BDAB3867
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115420 Genomic DNA. No translation available.
CH473964 Genomic DNA. Translation: EDM01922.1.
RefSeqiNP_001100089.1. NM_001106619.1.
UniGeneiRn.44078.

Genome annotation databases

EnsembliENSRNOT00000020785; ENSRNOP00000020785; ENSRNOG00000015409.
GeneIDi297593.
KEGGirno:297593.
UCSCiRGD:1308438. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115420 Genomic DNA. No translation available.
CH473964 Genomic DNA. Translation: EDM01922.1.
RefSeqiNP_001100089.1. NM_001106619.1.
UniGeneiRn.44078.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020785.

Protein family/group databases

MEROPSiC19.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020785; ENSRNOP00000020785; ENSRNOG00000015409.
GeneIDi297593.
KEGGirno:297593.
UCSCiRGD:1308438. rat.

Organism-specific databases

CTDi8078.
RGDi1308438. Usp5.

Phylogenomic databases

eggNOGiKOG0944. Eukaryota.
COG5207. LUCA.
GeneTreeiENSGT00390000000874.
KOiK11836.
OMAiGQRAYLH.
OrthoDBiEOG091G01W3.
TreeFamiTF300576.

Enzyme and pathway databases

ReactomeiR-RNO-5689880. Ub-specific processing proteases.
R-RNO-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Gene expression databases

BgeeiENSRNOG00000015409.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR015940. UBA.
IPR009060. UBA-like.
IPR016652. Ubiquitinyl_hydrolase.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiView protein in SMART
SM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiView protein in PROSITE
PS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiD3ZVQ0_RAT
AccessioniPrimary (citable) accession number: D3ZVQ0
Entry historyiIntegrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: March 15, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.