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Protein

Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66

Gene

No66

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation (By similarity).By similarity

Catalytic activityi

L-histidine-[60S ribosomal protein L8] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-histidine-[60S ribosomal protein L8] + succinate + CO2.
Protein N6,N(6)-dimethyl-L-lysine + 2-oxoglutarate + O2 = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO2.
Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O2 = protein L-lysine + succinate + formaldehyde + CO2.

Cofactori

Fe2+Note: Binds 1 Fe2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi296Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi298Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi361Iron; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Dioxygenase, Oxidoreductase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 (EC:1.14.11.-, EC:1.14.11.27)
Alternative name(s):
Histone lysine demethylase NO66
Gene namesi
Name:No66
Synonyms:Mapjd
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1307704. RGD1307704.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004176671 – 597Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66Add BLAST597

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei62PhosphoserineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Modified residuei86PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiD3ZU57.
PeptideAtlasiD3ZU57.
PRIDEiD3ZU57.

PTM databases

iPTMnetiD3ZU57.
PhosphoSitePlusiD3ZU57.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010126.
GenevisibleiD3ZU57. RN.

Interactioni

Subunit structurei

Interacts with SP7/OSX; the interaction is direct (By similarity). Interacts with MYC. Interacts with PHF19; leading to its recruitment to H3K36me3 sites (By similarity).By similarity

Protein-protein interaction databases

IntActiD3ZU57. 1 interactor.
STRINGi10116.ENSRNOP00000013431.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini250 – 395JmjCPROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ROX family. NO66 subfamily.Curated
Contains 1 JmjC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3706. Eukaryota.
ENOG410YNEJ. LUCA.
GeneTreeiENSGT00390000000083.
InParanoidiD3ZU57.
KOiK16914.
OMAiFEWLIAP.
OrthoDBiEOG091G04K2.
PhylomeDBiD3ZU57.
TreeFamiTF318659.

Family and domain databases

InterProiIPR003347. JmjC_dom.
[Graphical view]
PfamiPF08007. Cupin_4. 1 hit.
[Graphical view]
SMARTiSM00558. JmjC. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3ZU57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDELPNGNGA APLKRGRGRR RRQPQPRGAS VLALPLRPRK VRRHRKSAAS
60 70 80 90 100
RVAALRARAL LSEDSDSNVE SVRGKRERPA ELPEASRSAE PRPVPVRPRP
110 120 130 140 150
ASATLPRRVE GRAALSRNLG KPAPLPGSHV DDPERPWDSP LQQVLAELNG
160 170 180 190 200
IPSSRRRAAR LFEWLLAPLP PDHFYRRLWE REAVLVRRQD HSYYEGLFST
210 220 230 240 250
SDLDWMLRYE DVHFGQHLDA ARYIDGRRET LNPPGRALPA AAWSLYQAGC
260 270 280 290 300
SLRLLCPQAF SPTVWQFLAV LQEQFGSMAG SNVYLTPPNS QGFAPHYDDI
310 320 330 340 350
EAFVLQLEGR KLWRVYRPRD PSEELALTSS PNFSQEDLGE PVLQTVLEPG
360 370 380 390 400
DLLYFPRGFI HQAECQDGVH SLHLTLSTYQ RNTWGDFLEA VLPLAMQAAI
410 420 430 440 450
EENVEFRRGL PRDFMDYMGA QHSDSKDPRR TAFMEKVRVL VARLGHFAPV
460 470 480 490 500
DAVADQRAKD FIHDSLPPVL TDRERALSVH GLPIRWEAGE PVNVGAQLTT
510 520 530 540 550
ETQVHMLQDG IARLVGEGGR LFLYYTVENS RVYHLEEPKC LEIYPQQADA
560 570 580 590
MELLLRSYPE FVRVGDLPCD SVEDQLSLAT MLYDKGLLLT KTPLVLS
Length:597
Mass (Da):67,455
Last modified:April 20, 2010 - v1
Checksum:i0B6AF59002A0FCE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473982 Genomic DNA. Translation: EDL81467.1.
RefSeqiNP_001101510.1. NM_001108040.1.
UniGeneiRn.66167.

Genome annotation databases

EnsembliENSRNOT00000013431; ENSRNOP00000013431; ENSRNOG00000010126.
GeneIDi314300.
KEGGirno:314300.
UCSCiRGD:1307704. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473982 Genomic DNA. Translation: EDL81467.1.
RefSeqiNP_001101510.1. NM_001108040.1.
UniGeneiRn.66167.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiD3ZU57. 1 interactor.
STRINGi10116.ENSRNOP00000013431.

PTM databases

iPTMnetiD3ZU57.
PhosphoSitePlusiD3ZU57.

Proteomic databases

PaxDbiD3ZU57.
PeptideAtlasiD3ZU57.
PRIDEiD3ZU57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013431; ENSRNOP00000013431; ENSRNOG00000010126.
GeneIDi314300.
KEGGirno:314300.
UCSCiRGD:1307704. rat.

Organism-specific databases

CTDi314300.
RGDi1307704. RGD1307704.

Phylogenomic databases

eggNOGiKOG3706. Eukaryota.
ENOG410YNEJ. LUCA.
GeneTreeiENSGT00390000000083.
InParanoidiD3ZU57.
KOiK16914.
OMAiFEWLIAP.
OrthoDBiEOG091G04K2.
PhylomeDBiD3ZU57.
TreeFamiTF318659.

Miscellaneous databases

PROiD3ZU57.

Gene expression databases

BgeeiENSRNOG00000010126.
GenevisibleiD3ZU57. RN.

Family and domain databases

InterProiIPR003347. JmjC_dom.
[Graphical view]
PfamiPF08007. Cupin_4. 1 hit.
[Graphical view]
SMARTiSM00558. JmjC. 1 hit.
[Graphical view]
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNO66_RAT
AccessioniPrimary (citable) accession number: D3ZU57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: April 20, 2010
Last modified: November 2, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.