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Protein

Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha

Gene

Pip5k1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where it acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs (By similarity).By similarity

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1660499 Synthesis of PIPs at the plasma membrane
R-RNO-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha (EC:2.7.1.68)
Short name:
PIP5K1-alpha
Short name:
PtdIns(4)P-5-kinase 1 alpha
Alternative name(s):
68 kDa type I phosphatidylinositol 4-phosphate 5-kinase
Phosphatidylinositol 4-phosphate 5-kinase type I alpha
Short name:
PIP5KIalpha
Gene namesi
Name:Pip5k1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1306127 Pip5k1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004244371 – 546Phosphatidylinositol 4-phosphate 5-kinase type-1 alphaAdd BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki87Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiD3ZSI8
PeptideAtlasiD3ZSI8
PRIDEiD3ZSI8

PTM databases

iPTMnetiD3ZSI8
PhosphoSitePlusiD3ZSI8

Expressioni

Gene expression databases

BgeeiENSRNOG00000021068
GenevisibleiD3ZSI8 RN

Interactioni

Subunit structurei

Interacts with RAC1. Interacts with TUT1. Forms a complex with CDH1/E-cadherin, CTNNB1/beta-catenin and CTNND1 at the plasma membrane upon calcium stimulation (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028609

Structurei

3D structure databases

SMRiD3ZSI8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 433PIPKPROSITE-ProRule annotationAdd BLAST369

Phylogenomic databases

eggNOGiKOG0229 Eukaryota
COG5253 LUCA
GeneTreeiENSGT00760000119184
InParanoidiD3ZSI8
OMAiHMKYDLK
OrthoDBiEOG091G0A6L
PhylomeDBiD3ZSI8
TreeFamiTF319618

Family and domain databases

Gene3Di3.30.800.10, 1 hit
3.30.810.10, 2 hits
InterProiView protein in InterPro
IPR023610 PInositol-4-P-5-kinase
IPR027483 PInositol-4-P-5-kinase_C
IPR002498 PInositol-4-P-5-kinase_core
IPR027484 PInositol-4-P-5-kinase_N
PANTHERiPTHR23086 PTHR23086, 1 hit
PfamiView protein in Pfam
PF01504 PIP5K, 1 hit
SMARTiView protein in SMART
SM00330 PIPKc, 1 hit
PROSITEiView protein in PROSITE
PS51455 PIPK, 1 hit

Sequencei

Sequence statusi: Complete.

D3ZSI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASASSGPAA AGFSPLDSGV PAGTAASGIK RGTVSEGPYA SLMPVKKIGH
60 70 80 90 100
RSVDSSGETT YKKTTSSALK GAIQLGITHT VGSLSTKPER DVLMQDFYVV
110 120 130 140 150
ESIFFPSEGS NLTPAHHYND FRFKTYAPVA FRYFRELFGI RPDDYLYSLC
160 170 180 190 200
SEPLIELSNS GASGSLFYVS SDDEFIIKTV QHKEAEFLQK LLPGYYMNLN
210 220 230 240 250
QNPRTLLPKF YGLYCVQAGG KNIRIVVMNN LLPRSVKMHM KYDLKGSTYK
260 270 280 290 300
RRASQKEREK TLPTFKDLDF LQDIPDGLFL DADMYSALCK TLQRDCLVLQ
310 320 330 340 350
SFKIMDYSLL MSIHNMDHAQ REPMNSETQY SIDTRRPAPQ KALYSTAMES
360 370 380 390 400
IQGEARRGGT VETEDHMGGI PARNNKGERL LLYIGIIDIL QSYRFVKKLE
410 420 430 440 450
HSWKALVHDG DTVSVHRPGF YAERFQRFMC NTVFKKIPLK PSPTKKFRSG
460 470 480 490 500
PSFSRRSGPS GNSCTPSQPT ASGEHKAQVT TKAEVEPDIH LGRPDVLPQT
510 520 530 540
PPLEEISEGS PVPGPSFSPA VGQPLQILNL SSTLEKLDVA ESELTH
Length:546
Mass (Da):60,605
Last modified:April 20, 2010 - v1
Checksum:iF0DF7489A100D9C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY724476 mRNA No translation available.
AABR06019271 Genomic DNA No translation available.
UniGeneiRn.1836

Genome annotation databases

EnsembliENSRNOT00000028609; ENSRNOP00000028609; ENSRNOG00000021068
UCSCiRGD:1306127 rat

Similar proteinsi

Entry informationi

Entry nameiPI51A_RAT
AccessioniPrimary (citable) accession number: D3ZSI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: April 20, 2010
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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